Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Sadañoski, Marcela Alejandra; Benítez, Silvana Florencia; Fonseca, María Isabel; Velázquez, Juan Ernesto; Zapata, Pedro Darío; Levin, Laura; Villalba, Laura Lidia

doi:10.1016/j.jece.2019.103453

Cited by 35 publications

(16 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While most studies have explored the ability of ligninolytic Basidiomycete strains in the mycoremediation of PCB-polluted soils [ 2 , 4 , 14 , 15 , 31 ] and have shown in most cases underwhelming results [ 16 ], very few studies have investigated the potentiality of Ascomycete strains isolated from PCB-polluted soils to degrade these recalcitrant pollutants. These endogenous strains seemed to possess high potential capacities to degrade PCBs in liquid medium comparable to those of ligninolytic basidiomycete strains [ 17 , 21 , 22 , 32 ] and could potentially grow easily in their native soil without being hindered by competition with other native strains.…”

Section: Discussionmentioning

confidence: 99%

“…The use of fungal species could be a promising approach. Basidiomycete ligninolytic fungi, naturally associated with wood decay, are the most explored fungi in PCB remediation [ 2 , 14 , 15 ]. Despite their effectiveness in the PCB degradation in liquid medium, their PCB biodegradation rates in soils which are environmentally different from their natural habitats, were relatively low and seemed to be dependent on the soil PCB concentrations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Potentiality of Native Ascomycete Strains in Bioremediation of Highly Polychlorinated Biphenyl Contaminated Soils

et al. 2021

View full text Add to dashboard Cite

Polychlorinated biphenyls (PCBs) are organic pollutants that are harmful to environment and toxic to humans. Numerous studies, based on basidiomycete strains, have reported unsatisfactory results in the mycoremediation of PCB-contaminated soils mainly due to the non-telluric origin of these strains. The abilities of a five-Ascomycete-strain consortium in the mycoremediation of PCB-polluted soils and its performance to restore their sound functioning were investigated using mesocosm experiments associated with chromatography gas analysis and enzymatic activity assays. With the soil H containing 850 ppm PCB from which the strains had been isolated, a significant PCB depletion of 29% after three months of treatment was obtained. This led to an important decrease of PCBs from 850 to 604 ppm. With the soil L containing 36 ppm PCB, biodegradation did not occur. In both soils, the fungal biomass quantified by the ergosterol assay, did not increase at the end of the treatment. Biodegradation evidenced in the soil H resulted in a significantly improved stoichiometry of N and P acquiring enzymatic activities. This unprecedented study demonstrates that the native Ascomycetes display remarkable properties for remediation and restoration of functioning of the soil they originated from paving the way for greater consideration of these strains in mycoremediation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Potentiality of Native Ascomycete Strains in Bioremediation of Highly Polychlorinated Biphenyl Contaminated Soils

et al. 2021

View full text Add to dashboard Cite

show abstract

“…This strain was selected due to its high sensitivity towards PCBs in previous studies (Sadañoski et al . 2018, 2019). Microbial toxicity was evaluated in Petri dishes containing 12 ml of supplemented sugarcane bagasse culture media (Sadañoski et al .…”

Section: Methodsmentioning

confidence: 99%

“…Strains belonging to the genera of Pleurotus and Trametes demonstrated to be promising for mycoremediation of PCBs (Sadañoski et al . 2018, 2019, 2020a, 2020b). For instance, in previous studies, PCB contaminated soil treated with Pleurotus pulmonarius LBM 105 and Trametes sanguinea LBM 023 immobilized on sugarcane bagasse showed higher enzyme activities and detoxification levels when the strains were applied in co‐culture (Sadañoski et al .…”

Section: Introductionmentioning

confidence: 99%

Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

et al. 2021

Self Cite

View full text Add to dashboard Cite

Aims To evaluate the mycoremediation of polychlorinated biphenyls (PCBs) by either single cultures or binary consortia of Pleurotus pulmonarius LBM 105 and Trametes sanguinea LBM 023. Methods and Results PCBs tolerance, removal capacity, toxicity reduction and ligninolytic enzyme expression were assessed when growing single culture and binary consortium of fungus in 217 mg l−1 of a technical mixture of Aroclor 1242, 1254 and 1260 in transformer oil. A decrease in tolerance and variation in ligninolytic enzyme secretion were observed in PCB‐amended solid media. Pleurotus pulmonarius LBM 105 mono‐culture was able to remove up to 95·4% of PCBs, whereas binary consortium and T. sanguinea LBM 023 could biodegrade about 55% after 24 days. Significant detoxification levels were detected in all treatments by biosorption mechanism. Conclusions Pleurotus pulmonarius LBM 105 in single culture had the best performance regarding PCBs biodegradation and toxicity reduction. Ligninolytic enzyme secretion changed in co‐culture. Significance and Impact of the Study The evaluation of PCBs bioremediation effectiveness of basidiomycetes consortium in terms of PCB removal, toxicity and ligninolytic enzyme production to unravel the differences between using individual cultures or consortium has not been reported. The results from this study enable the selection of P. pulmonarius LBM 105 mono‐culture to bioremediate PCBs as it showed higher efficiency compared to binary consortium with T. sanguinea LBM 023 for potential decontamination of PCB‐contaminated transformer oil.

show abstract

“…For the SEM analysis, myceliums which were 10-dayold were separated from the supernatant. The hyphae were then fixed with a solution containing formaldehyde, alcohol and acetic acid (10 : 50 : 5) (Sadañoski et al 2019). After drying at critical point with CO 2 and goldmetalized, the surface was observed with a scanning electron microscope JEOL 5800 LV from JEOL Ltd, Tokyo, Japan.…”

Section: Fungal Biomass and Ultramorphological Analysismentioning

confidence: 99%

Bioproduction of α‐terpineol and R‐(+)‐limonene derivatives by terpene‐tolerant ascomycete fungus as a potential contribution to the citrus value chain

Velázquez

Sadañoski

Zapata

et al. 2020

J. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Aims: The aims of this article were to select fungal species with high tolerance and high growth rate in mediums supplemented with limonene and citrus essential oils (CEOs), and to test the bioconversion capability of the chosen isolates for the bioproduction of aroma compounds. Methods and Results: Based on the use of predictive mycology, 21 of 29 isolates were selected after assaying R-(+)-limonene and CEO tolerance (10 g l −1). With a dendrogram divisive coefficient of 0Á937, the subcluster two with isolates Aspergillus niger LBM 055, Penicillium sp. LBM 150, Penicillium sp. LBM 151 and Penicillium sp. LBM 154 gathered the highest tolerance and mycelia growth speed. Ultrastructural analysis indicated that culture media containing limonene had no visible toxic activity that could promote morphological changes in the fungal cell wall. The biomass of A. niger LBM055 was distinctive in liquid media supplemented with R-(+)-limonene (0Á57 AE 0Á07 g) and it was selected to prove bioconversion capacity, under static and agitated conditions, and converted up to 98% of limonene, yielding a wide variety of products that were quantified by GC-FID. It was obtained at molecular weights less than limonene (64-100%), between limonene and αterpineol (12-72%) and greater than α-terpineol (2-48%). Conclusions: Aspergillus niger LBM 055, Penicillium sp. LBM 150, Penicillium sp. LBM 151 and Penicillium sp. LBM 154 showed to the highest tolerance and growth rate in mediums supplemented with R-(+)-limonene and orange and lemon essential oils. Particularly, A. niger LBM055, showed limonene bioconversion capability and produced different molecular weights compounds such us α-terpineol. Significance and Impact of the Study: Different bioproducts can be obtained by changing operative condition with the same fungus, and this bioprocess aspect is a significant approach to be adopted on industrial scale leading to the creation of new natural flavours and fragrance compositions.

show abstract

Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Cited by 35 publications

References 62 publications

Potentiality of Native Ascomycete Strains in Bioremediation of Highly Polychlorinated Biphenyl Contaminated Soils

Potentiality of Native Ascomycete Strains in Bioremediation of Highly Polychlorinated Biphenyl Contaminated Soils

Comparative study of single cultures and a consortium of white rot fungi for polychlorinated biphenyls treatment

Bioproduction of α‐terpineol and R‐(+)‐limonene derivatives by terpene‐tolerant ascomycete fungus as a potential contribution to the citrus value chain

Contact Info

Product

Resources

About