Mycoremediation Potential of Synthetic Textile Dyes by Aspergillus niger via Biosorption and Enzymatic Degradation

Abstract

Textile dyes that persist in the environment are highly resistant to the natural degradation processes that occur in the environment. Therefore, the present study isolated, identified, and optimized textile dye decolorization by fungi and elucidated the dye decolorization pathway to develop a low-cost biotechnological approach for decolorization and detoxification of textile dyes. Within 36 hours of incubation at temperatures ranging from 28 to 40°C, pH 7, and shaking at 100 rpm, Aspergillus niger MN990895, which was selected from a total of 77 fungal isolates, completely decolorized the model dye CI Direct Blue 201 (DB 201). A. niger biosorbed 8.4±1.2% of the dye used where live biomass showed complete dye removal. It was found that extracellular crude enzymes were more involved in DB 201 dye decolorization (72.7±3.3%) than intracellular crude enzymes. The enzymatic studies suggested that the primary enzyme involved in DB 201 textile dye decolorization was lacccase, which was further confirmed by the presence of distinct protein bands around 75-100 kDa on the SDS-PAGE. The FTIR spectra and seed germination assays confirmed that A. niger proved successful in DB 201 textile dye degradation and detoxification. The present study suggests that A. niger may have promising implications in the future for the development of an enzyme-based textile wastewater treatment system.

Potential of White Rot Fungi from Berbak-Sembilang National Park, Indonesia for Decolorization and Detoxification Commercial Direct Dyes

“…So, it is possible that other enzymes are involved. As reported by Ekanayake and Manage [24] that A. niger can produce ligninolytic enzymes, namely LP, MnP, tyrosine, laccase and azoreductase during the textile dye decolorization process. Another possible mechanism that could occur is biosorption of fungal mycelium [23].…”

“…This mechanism often occurs in fungi because it can produce several non-specific extracellular and intracellular enzymes involved in the process of removing dyes. Research results by Ekanayake and Manage [24] stated that extracellular enzymes were significantly more involved in the decolorization of CI Direct Blue 201. In addition, BRB 74 and BRB 81 have low enzyme activities, and no increase in biomass but decolorization can reach 50 and 33 %, respectively.…”

An exploration of unseen fungal world and analysis of their functional attributes in crop and human health

“…Laccase was shown to be the major enzyme involved in the decolorization of DB 201 textile dyes, which was validated by the appearance of discrete protein bands about 75-100 kDa on SDS-PAGE. The FTIR spectra and seed germination experiments both revealed that A. niger was effective at degrading and detoxifying the DB 201 textile dye (Ekanayake and Manage, 2022). Within 14 days, Bjerkandera adusta showed the best decolorization for the TPM dyes.…”

Coagulation Study on Extracted Algal Alginate from Red Algae as Natural Coagulant for Remediation of Textile Dye Congo Red

“…The other factors like double layer compression, sweep flocculation and charge neutralization may influence a little. However, this bio coagulant, capable of producing a gel, will follow the intraparticle bridging mechanism commonly known as the Egg box model (Ekanayake and Manage, 2022). The presence of carboxyl groups with double bonds increases the affinity of the dye particle towards the coagulant.…”

“…The monomers create MM, MG, and GG block structures to create alginate. According to Aylin Devrimci (Devrimci et al, 2012;Ekanayake and Manage, 2022), virtual quantity of these building blocks present in alginate structure shows better performance with metal ions. Alginate is the only polysaccharide, which naturally contains carboxyl groups in each constituent residue, and possesses various abilities for functional materials (Ikeda et al, 2000).…”