Mycorrhiza consortia suppress the fusarium root rot ( Fusarium solani f. sp. Phaseoli ) in common bean ( Phaseolus vulgaris L.)

Eke, Pierre; Chatue, Gael Chatue; Wakam, Louise Nana; Toghueo, Rufin Marie Kouipou; Fokou, Patrick Valère Tsouh; Boyom, Fabrice Fekam

doi:10.1016/j.biocontrol.2016.10.001

Cited by 57 publications

(28 citation statements)

References 34 publications

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“…In the present study, Xac infection induced an increase in root PAL activity and in lignin and total soluble phenol concentrations in the treated plants. These findings are consistent with those from bean (Eke et al ) and cotton (Xu et al ). In addition, the healthy neighboring seedlings that were linked with the Xac‐infected treated seedlings, via CMNs, corresponding to the +AMF+Xac treatment, had higher root PAL activity and higher total root soluble phenol and lignin concentrations than seedlings subjected to the +AMF−Xac treatment, which can effectively prevent pathogen infection (Vorwerk et al ; Sarkar et al ).…”

Section: Discussionsupporting

confidence: 92%

Common mycorrhizal networks activate salicylic acid defense responses of trifoliate orange (Poncirus trifoliata)

Zhang

Zou

Liu

et al. 2019

JIPB

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Citrus canker, caused by Xanthomonas axonopodis pv. citri (‘Xac’), is an important quarantine disease in citrus crops. Arbuscular mycorrhizal fungi (AMF) form symbiotic interactions with host plants and further affect their disease resistance, possibly by modulating the activity of salicylic acid (SA), a key phytohormone in disease resistance. Common mycorrhizal networks (CMNs) can interconnect plants, but it is not yet clear whether CMNs promote resistance to citrus canker and, if so, whether SA signaling is involved in this process. To test this possibility, we used a two‐chambered rootbox to establish CMNs between trifoliate orange (Poncirus trifoliata) seedlings in chambers inoculated (treated) or not (neighboring) with the AMF, Paraglomus occultum. A subset of the AMF‐inoculated seedlings were also inoculated with Xac (+AMF+Xac). At 2 d post‐inoculation (dpi), compared with the +AMF−Xac treatment, neighboring seedlings in +AMF+Xac treatment had lower expression levels of the SA biosynthetic genes, PtPAL, PtEPS1, and PtPBS3, but higher SA levels, which attributed to the upregulation of PtPAL and PtPBS3 in treated seedlings and the transfer of SA, via CMNs, to the neighboring seedlings. At 4 dpi, the pathogenesis‐related (PR) protein genes, PtPR1, PtPR4, and PtPR5, and the transcriptional regulatory factor gene, PtNPR1, were activated in neighboring seedlings of +AMF+Xac treatment. At 9 dpi, root phenylalanine ammonia‐lyase activity and total soluble phenol and lignin concentrations increased in neighboring seedlings of +AMF+Xac treatment, likely due to the linkage and signal transfer, via CMNs. These findings support the hypothesis that CMNs transfer the SA signal from infected to neighboring healthy seedlings, to activate defense responses and affording protection to neighboring plants against citrus canker infection.

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Section: Discussionsupporting

confidence: 92%

Common mycorrhizal networks activate salicylic acid defense responses of trifoliate orange (Poncirus trifoliata)

Zhang

Zou

Liu

et al. 2019

JIPB

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“…In our study, we found that the disease index of soybean root rot was significantly higher in control non-inoculated with F. mosseae than that in the treatment, indicating that inoculation of F. mosseae could significantly improve the resistance of soybean to soil-borne pathogens, ultimately alleviated the occurrence of soybean root rot. Previous studies have indicated that AM fungi could significantly inhibit some soil-borne pathogens, such as species of Phytophthora, Rhizoctonia and Fusarium (Abdel-Fattah et al, 2011;Sukhada et al, 2011;Eke et al, 2016). In our previous study, we have shown that species of Fusarium are the main parasitic pathogens of soybean root rot in Northeast China.…”

Section: Discussionmentioning

confidence: 59%

Community composition of rhizosphere fungi as affected by Funneliformis mosseae in soybean continuous cropping soil during seedling period

Jie

Lin

Guo

et al. 2019

Chil. j. agric. res.

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Arbuscular mycorrhizal (AM) fungi can enhance plant resistance particularly against soil-borne pathogenic fungi. However, little is known about the effects of Funneliformis mosseae on the community composition of rhizosphere fungi in soybean (Glycine max [L.] Merr.) continuous cropping soil. Here, the disease index of soybean root rot was analyzed, and high throughput sequencing technology was applied to investigate whether F. mosseae could change the composition of fungal communities in the rhizosphere of continuous cropping soybean during seedling period. The results indicated that the disease index of soybean root rot decreased significantly after inoculation of F. mosseae. The root rot disease index was also affected by the increasing of continuous cropping regimes. Furthermore, the relative abundance of fungal community in soybean rhizosphere soil and root samples was influenced after inoculation. Ascomycota was the dominant phylum in most samples. Basidiomycota was the second dominant phylum in all the soil samples, but Olpidiomycota was the second phylum in most root samples. At the genus level, both inoculation and continuous cropping regimes had significant effects on the dominant genus and their relative abundances in all the samples. The relative abundance of some plant pathogenic fungi such as Fusarium in the inoculated root samples was lower than those in the non-inoculated root samples in the same continuous cropping regime. The results can provide new insights into the interactive effects of AM fungi and rhizosphere fungi, and also provide theoretical evidence on biological solutions to alleviate the obstacles of soybean continuous cropping.

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“…In general, suppressing the growth of pathogen fungi by mycorrhizae is most likely based on complex processes, where root and hyphae exudates may play a crucial role [29,31]. Lioussanne et al [26] showed that at an early stage of mycorrhizal colonization attractively effects on zoospore germination of Phytopthora nicotianae (Peronosporales: Peronosporaceae) while only root exudates from plant roots that are extensively colonized by AM fungi show inhibitory effects.…”

Section: Discussionmentioning

confidence: 99%

“…This beneficial effect of AMF can be attributed to the dramatically altered plant primary and secondary metabolism in affected roots, thereby influencing microorganisms living in the rhizosphere [23,[25][26][27][28]. The decreased growth of filamentous fungi in the presence of AMF has already been investigated [29][30][31][32] but how its mycotoxin producing ability works is not well known. Ismail et al [33] covered the opportunities of using mycorrhizal fungi to control fumonisin production, but there is no data regarding Fusarium proliferatum.…”

Section: Introductionmentioning

confidence: 99%

Mycorrhizal Root Exudates Induce Changes in the Growth and Fumonisin Gene (FUM1) Expression of Fusarium proliferatum

2019

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In this study, root exudates from mycorrhizal and non-mycorrhizal plants growing at low or high nutrient supply were used in vitro to examine their effects on the growth and fumonisin B1 gene (FUM1) expression of Fusarium proliferatum (Hypocreales: Nectriaceae). After one day of exposure to root exudates originating from non-mycorrhizal and low nutrient supply treatment, a significant change in the growth of F. proliferatum was measured, which then equalized after 5 days of incubation. Aside from the fumonisin gene (FUM1) gene, the expression of the mitogen-activated protein kinase gene (HOG1) was also studied using quantitative real-time polymerase chain reaction (qRT-PCR). After 5 days of incubation, mycorrhizal root exudates significantly reduced the expression of the FUM1 gene, irrespective of the extent of the nutrient supplement and colonization level of the target plant. Similar trends in the expressions of FUM1 and HOG1 genes found in our experiment suggest that arbuscular mycorrhizal fungal colonization did not only affect directly the growth and mycotoxin production of F. proliferatum, but also modulated indirectly a number of other mechanisms. Mycorrhizal inoculation showed potential as a biological control agent in the suppression of fumonisin production by F. proliferatum.Root exudates were collected from maize plants treated, as described previously, by removing all substrate particles from the roots and then submerging them into 50 mL of 0.01 M L −1 KOH according to da Silva Lima et al. [38]. After 5 min, the root system was washed with tap water, then with Agronomy 2019, 9, 291 3 of 11 distilled water and were incubated in Erlenmeyer flasks filled with 50 mL sterilized distilled water for 24 h. Solutions were sterilized by filtration through 0.22 µm Ø nitrocellulose filters (Millipore, Burlington, MA, USA). Concentrations of root exudates were adjusted to a ratio of 1 g of root fresh weight based on the method of Lioussanne et al. [39]. In this way, the effects of various root exudates remained comparable, however, roots from different treatments showed significant differences in weights. The root exudates were kept at −20 • C until use. Culture Conditions and Expression Analyses of FUM1 and HOG1 GenesFusarium proliferatum ITEM 2287 (Institute of Sciences of Food Production, CNR, Bari, Italy) was used in the study. Mycelium for fumonisin production was prepared in 50 mL DM (22 mM KH 2 PO 4 , 2.5 mM MgSO 4 , 85 mM NaCl and 117 mM sucrose, pH 5.9) according to Shim and Woloshuk [40], supplemented with 30 mM ammonium dihydrogen phosphate and inoculated with 10 6 mL −1 conidia. Cultures were incubated at 26 • C with shaking (150 rpm) for 48 h then filtrated and washed with sterilized distilled water and mycelium, then transferred directly to 50 mL of new DM medium. The co-cultures were inoculated separately with 5 mL (concentrations adjusted to a ratio of 1 g of root fresh weight) of each of root exudate (+AM LN, −AM LN, +AM HN, −AM HN). The control treatment was prepared by adding 5 mL of sterilized ...

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Mycorrhiza consortia suppress the fusarium root rot ( Fusarium solani f. sp. Phaseoli ) in common bean ( Phaseolus vulgaris L.)

Cited by 57 publications

References 34 publications

Common mycorrhizal networks activate salicylic acid defense responses of trifoliate orange (Poncirus trifoliata)

Common mycorrhizal networks activate salicylic acid defense responses of trifoliate orange (Poncirus trifoliata)

Community composition of rhizosphere fungi as affected by Funneliformis mosseae in soybean continuous cropping soil during seedling period

Mycorrhizal Root Exudates Induce Changes in the Growth and Fumonisin Gene (FUM1) Expression of Fusarium proliferatum

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