Mycorrhizal fungi and growth and development of micropropagated Scutellaria integrifolia plants

Joshee, Nirmal; Mentreddy, Srinivasa Rao; Yadav, Animesh

doi:10.1016/j.indcrop.2006.08.009

Cited by 33 publications

(26 citation statements)

References 22 publications

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“…BAP has been reported to be more efficient at inducing shoot proliferation than other cytokinins in many other plants, including some Scutellaria species. Joshee et al (2007) found the maximum number of shoots from shoot tips of S. integrifolia (23 per explant) to be obtained on MS medium supplemented with 2.2 lM BAP, while for S. discolor the best results were obtained in the presence of 4.4 lM BAP (Sinha et al 1999). Also, the increase in fresh and dry biomass of S. alpina shoots grown for 5 weeks on media containing BAP was largest, although the shoots were shorter than those grown in the presence of other tested cytokinins, especially kinetin and zeatin, and those cultured on control medium (MS without cytokinin).…”

Section: Discussionmentioning

confidence: 99%

The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina

Grzegorczyk-Karolak

Kuźma

Wysokińska

2015

Plant Cell Tiss Organ Cult

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The present study evaluates the effects of various cytokinins on Scutellaria alpina shoot proliferation and production of polyphenolic metabolites (baicalin, wogonoside, luteolin, luteolin 7-O-glucoside, verbascoside). The shoots were induced from shoot tips on MS medium supplemented with IAA (indole-3-acetic acid, 0.57 lM) and various concentrations of 6-benzylaminopurine (BAP), kinetin, zeatin (1, 2, 4, 8 lM) or tidiazuron (TDZ) (0.2, 0.5, 1 lM). Among the cytokinins tested, BAP was the most effective for shoot induction, and the highest number of shoots (25 per explant) was achieved with 2 and 4 lM BAP. Maximum biomass production was also achieved on these media. Significantly higher baicalin, wogonoside and verbascoside contents were recorded in treatments containing cytokinins combined with 0.57 lM IAA, when compared to cytokinin-free medium. TDZ at a concentration of 0.5 lM was the most effective for polyphenol production. However, supplementation with cytokinins often results in the reduction of luteolin and its 7-O-glucoside production in the shoot culture of S. alpina. ABTS [2,20 -azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)] and ferric reducing antioxidant power assays were used to identify the antioxidant potential of methanolic extracts from shoots cultured in the presence of different types and concentrations of cytokinins. In both tests, the shoots from medium supplemented with 0.5 lM TDZ demonstrated the strongest antioxidant activity. The results indicate that higher polyphenolic content correlated with greater reducing power and antiradical efficiency.

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Section: Discussionmentioning

confidence: 99%

The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina

Grzegorczyk-Karolak

Kuźma

Wysokińska

2015

Plant Cell Tiss Organ Cult

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“…The establishment of in vitro grown seedlings in soil is hampered by weak root system at the beginning of acclimation, however, the symbiotic association between AMF and plant roots increases the survival rate of plant to strengthen the root system (Yadav et al, 2012). This strengthening can reflect the importance of AMF for nutrients and water uptake at low fertilized environments, defense against pathogens, decreased water stress improving some important characteristics for plant acclimation (Joshee et al, 2007;Pindi, 2011;Singh et al, 2012;Yadav et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effect of arbuscular mycorrhizal fungi on survival and growth of micropropagated Comanthera mucugensis spp. mucugensis (Eriocaulaceae)

Pereira¹,

Santos²,

Alvim³

et al. 2017

Afr. J. Agric. Res.

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The use of micropropagation technique has been an alternative to conservation of endangered species, Comanthera mucugensis subsp. mucugensis (popular namely sempre viva de Mucuge); however, there is no information on the effect of arbuscular mycorrhizal fungi (AMF) on the acclimation process of micropropagated plants. This study evaluated the survival, growth and nutritional aspects of the species, C. mucugensis subsp. mucugensis inoculated with native AMFs in greenhouse condition. The design of the experiment consisted initially of 80 sampling units divided into four treatments: plants inoculated with native AMF, with microbiota filtrate from soil, with AMF plus filtrate and control (noninoculated plants). At three and eleven-month-old, the plants were collected for evaluation of growth, nutrition and mycorrhizal colonization. After eleven months of experiment, survival rate of AMF and AMF plus filtrate plants were 62.5 and 87.5%, respectively, and only one microbiota filtrate and one control plants survived. AMF inoculation also provided increase in n dry matter of rosettes and permitted obtaining flowering ten-month-growth plants. Rates of mycorrhizal colonization were high at three (aproximately 64.9%) and eleven (aproximately 94.5%) months for AMF and AMF plus filtrate plants. Number of spores in rhizosphere soil of mycorrhizal plants was also high (1599 per 100 dm 3 of soil) and seven diferent species of AMF were identified at the end of experiment. Data set evidenced mycortrophic character of C. mucugensis subsp. mucugensis and the importance of AMF inoculation for acclimation and survival of microprogagated plants which is essential for conservation of this endangered plant.

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“…bicolor using modifi ed MS medium & TDZ also evaluated effects of TDZ treatments for different durations of time in MS medium (containing MS salts, B5 vitamin and 3% sucrose), to induce regeneration. Similarly, shoot and callus culture techniques have also been used to observe phenolic compound in S. orientalis, S. baicalensis, S. iyoensis, S. taurica, S. laterifl ora, S. ventenatii, S. pontica, S. taurica, S. galericulata, S. integrifolia and S. alpina (Nishikawa et al 1999, Hirotani et al 1998, Zobayed et al 2004and Joshee et al 2007.…”

Section: Abstract R E G U L a R A R T I C L Ementioning

confidence: 99%

Micropropagation of Endemic Scutellaria orientalis L. subsp. Bicolor Using Modified MS Medium and TDZ

Özdemir

Yildirim

Kahriz

2015

Emir. J. Food Agric

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Mycorrhizal fungi and growth and development of micropropagated Scutellaria integrifolia plants

Cited by 33 publications

References 22 publications

The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina

The effect of cytokinins on shoot proliferation, secondary metabolite production and antioxidant potential in shoot cultures of Scutellaria alpina

Effect of arbuscular mycorrhizal fungi on survival and growth of micropropagated Comanthera mucugensis spp. mucugensis (Eriocaulaceae)

Micropropagation of Endemic Scutellaria orientalis L. subsp. Bicolor Using Modified MS Medium and TDZ

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