Mycotoxin production by Alternaria strains isolated from Argentinean wheat

Patriarca, Andrea; Azcarate, Mariela Pamela; Terminiello, Laura; Pinto, Virginia Fernández

doi:10.1016/j.ijfoodmicro.2007.07.055

Cited by 102 publications

(85 citation statements)

References 16 publications

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“…The high number of Alternaria isolations in the current study is similar to Patriarca et al (2007) and Kulik et al (2014) who found that Alternaria spp. were common spoilage fungi of crops in the field and caused postharvest decomposition of various grains including wheat and rye in Poland.…”

Section: Discussionsupporting

confidence: 91%

Fungal contaminants of stored wheat vary between Australian states

Barkat

Hardy

Ren

et al. 2016

Australasian Plant Pathol.

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A survey was undertaken to determine the mycoflora associated with stored wheat in four states across Australia including Western Australia, South Australia, Victoria and New South Wales. A total of 482 fungal isolates from 15 genera were isolated. The most common genera isolated were Alternaria spp., Aspergillus sp., Aureobasidium sp., Cladosporium spp., Drechslera sp., Fusariu m spp., Mucor sp., Nigrospora sp., Penicillium sp., Rhizopus sp., Stemphylium sp., Eutiarosporella sp p., Ulocladium sp., Epicoccum sp., and an undescribed genus from the Hypocreales. Diversity profiling also identified fungi that were not isolated using traditional methods including Paecilomyces spp., Dendryphiella and Cryptococcus. The results indicate that the mycoflora of stored wheat varies between Australian grain growing regions, and that diversity profiling analysis identifies different fungal contaminants compared to traditional methods. This study also revealed that some pathogens of high risk to humans (e.g. Cryptococcus) may occur with a high frequency in wheat.

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Section: Discussionsupporting

confidence: 91%

Fungal contaminants of stored wheat vary between Australian states

Barkat

Hardy

Ren

et al. 2016

Australasian Plant Pathol.

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“…TA was the toxin produced at the highest concentration. This result demonstrates a lower production of Alternaria toxins compared to strains isolated from different substrates as Argentine wheat, blueberries and tomatoes [34,[43][44][45].…”

Section: Discussionmentioning

confidence: 78%

“…This substrate is suitable for the mycotoxins biosynthesis because mechanical barriers and grains defenses, which are thermolabile, have been suppressed, thus allowing Alternaria isolates to express their toxicogenic potential in the substrate analyzed [43]. It is necessary to evaluate the toxicogenic capacity in grapes or a media that representing this substrate to know the real potential risk of these mycotoxins.…”

Section: Discussionmentioning

confidence: 99%

Determination of mycotoxin profiles characteristic ofAlternariastrains isolated from Malbec grapes

Trinidad

Ganoza

Pinto

et al. 2015

BIO Web of Conferences

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Abstract. The world grape production has increased, reaching 751 million quintals (Mql) in 2013. Many Alternaria species have been studied for their ability to produce secondary metabolites in foods, some of which have toxic properties with tenuazonic acid (TA), alternariol (AOH), alternariol methyl ether (AME) being the most important ones. The aim was to determine the characteristic mycotoxin production profiles of Alternaria strains isolated from Malbec grapes in the Patagonian region of Argentina. Fifty Alternaria isolates (5 A. alternata, 5 A. arborescens and 40 A. tenuissima) were analyzed for the production of mycotoxins (TA, AOH and AME) in autoclaved rice media by High Performance Liquid Chromatography (HPLC). All isolates were found to be producers of mycotoxins; the 100% was producer of TA (0.016-21.031 mg/kg), 98% produced AOH (0.003-0.057 mg/kg) and 36% produced AME (0.001-0.133 mg/kg). Thirty-three isolates co-produced the three mycotoxins. In this study, it was demonstrated a high toxigenic potential of Alternaria isolates. Although Alternaria growth on grapes has been amply demonstrated, there are few studies about the incidence their more characteristic mycotoxin sand their toxicogenic capacity determination in grapes, wines and derivatives. In addition, mycotoxins studied in this work are not regulated in oenology. Therefore, further studies should be conducted to assess the health risk due to the presence of Alternaria toxins in grapes, wine, grape juice and raisins.

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“…The genus Alternaria are wildly distributed in agricultural products such as wheat, tomatoes and apple juice (1,2). According to their different structures, mycotoxins of alternaria are classified into three types: tetramic acid derivatives, dibenzopyrone derivatives and perylene derivatives (2,3).…”

Section: Introductionmentioning

confidence: 99%

“…According to their different structures, mycotoxins of alternaria are classified into three types: tetramic acid derivatives, dibenzopyrone derivatives and perylene derivatives (2,3). Alternariol (AOH) is one of the important secondary products, belonging to dibenzopyrone derivatives, which is widely investigated on different aspects.…”

Section: Introductionmentioning

confidence: 99%

Alternariol induces DNA polymerase β expression through the PKA-CREB signaling pathway

et al. 2012

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Abstract. Alternariol (AOH) is a mycotoxin of Alternaria alternata and can cause DNA damage and gene mutations. Low-dose and long-term treatment with AOH has been linked with incidence of esophageal carcinoma. DNA polymerase β (polβ) is a key enzyme in DNA base excision repair (BER). When it is overexpressed or mutated in cells, DNA polβ can cause genetic instability. Elevated DNA polβ has also been reported in several human cancers. Here, we report that AOH at 2, 10, 20 µM induces DNA polβ expression. In the process, protein kinase A (PKA) catalytic subunit activation, nuclear translocation and cAMP response element binding protein (CREB) phosphorylation are involved. AOH also increased CREB binding to the cAMP response element (CRE) consensus motif, which is present in the DNA polβ gene promoter. The PKA inhibitor H89 was able to block AOH-induced PKA-CREB activation, CREB DNA binding activity and decrease DNA polβ expression. Our results suggest that AOH can upregulate DNA polβ expression through the PKA-CREB signal transduction pathway.

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Mycotoxin production by Alternaria strains isolated from Argentinean wheat

Cited by 102 publications

References 16 publications

Fungal contaminants of stored wheat vary between Australian states

Fungal contaminants of stored wheat vary between Australian states

Determination of mycotoxin profiles characteristic ofAlternariastrains isolated from Malbec grapes

Alternariol induces DNA polymerase β expression through the PKA-CREB signaling pathway

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