MyDEP: A New Computational Tool for Dielectric Modeling of Particles and Cells

We present a microfluidic platform allowing dielectrophoresis‐assisted formation of cell aggregates of controlled size and composition under flow conditions. When specific experimental conditions are met, negative dielectrophoresis allows efficient concentration of cells towards electric field minima and subsequent aggregation. This bottom‐up assembly strategy offers several advantages with respect to the targeted application: first, dielectrophoresis offers precise control of spatial cell organization, which can be adjusted by optimizing electrode design. Then, it could contribute to accelerate the establishment of cell‐cell interactions by favoring close contact between neighboring cells. The trapping geometry of our chip is composed of eight electrodes arranged in a circle. Several parameters have been tested in simulations to find the best configurations for trapping in flow. Those configurations have been tested experimentally with both polystyrene beads and human embryonic kidney cells. The final design and experimental setup have been optimized to trap cells and release the created aggregates on demand.

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“…As can be seen on the plots of

R e [C M false(f false)]

(see Supporting Information Figures S1 and S2, obtained using the MyDEP software ), up to 10 kHz the dielectrophoretic force will be maximal. Thus

σ_{m} = 0.156 S / m

was selected as well as

f_{D E P} = 10 k H z

.…”

Section: Resultsmentioning

confidence: 91%

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

et al. 2019

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“…We calculated the CM factor for each studied type of particle (beads of both sizes and yeast cells) using the MyDEP software and experimental parameters obtained previously (see Supporting Information ). Results are displayed as a function of the frequency on Fig.…”

Section: Resultsmentioning

confidence: 99%

Performance improvement of plasmonic sensors using a combination of AC electrokinetic effects for (bio)target capture

et al. 2019

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Analytes concentration techniques are being developed with the appealing expectation to boost the performance of biosensors. One promising method lies in the use of electrokinetic forces. We present hereafter a new design for a microstructured plasmonic sensor which is obtained by conventional microfabrication techniques, and which can easily be adapted on a classical surface plasmon resonance imaging (SPRI) system without further significant modification. Dielectrophoretic trapping and electro‐osmotic displacement of the targets in the scanned fluid are performed through interdigitated 200 μm wide gold electrodes that also act as the SPR‐sensing substrate. We demonstrate the efficiency of our device's collection capabilities for objects of different sizes (200 nm and 1 μm PS beads, as well as 5–10 μm yeast cells). SPRI is relevant for the spatial analysis of the mass accumulation at the electrode surface. We demonstrate that our device overcomes the diffusion limit encountered in classical SPR sensors thanks to rapid collection capabilities (<1 min) and we show a consequent improvement of the detection limit, by a factor >300. This study of an original device combining SPRI and electrokinetic forces paves the way to the development of fully integrated active plasmonic sensors with direct applications in life sciences, electrochemistry, environmental monitoring and agri‐food industry.

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“…A and B. Figure C and D represent the CM factor for 3 μm latex beads and HEK293 cells calculated with MyDEP software . Electric properties of HEK293 cells present in the MyDEP database were used .…”

Section: Resultsmentioning

confidence: 99%

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

et al. 2019

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The performance of conventional surface plasmon resonance (SPR) biosensors can be limited by the diffusion of the target analyte to the sensor surface. This work presents an SPR biosensor that incorporates an active mass‐transport mechanism based on dielectrophoresis and electroosmotic flow to enhance analyte transport to the sensor surface and reduce the time required for detection. Both these phenomena rely on the generation of AC electric fields that can be tailored by shaping the electrodes that also serve as the SPR sensing areas. Numerical simulations of electric field distribution and microparticle trajectories were performed to choose an optimal electrode design. The proposed design improves on previous work combining SPR with DEP by using face‐to‐face electrodes, rather than a planar interdigitated design. Two different top‐bottom electrode designs were experimentally tested to concentrate firstly latex beads and secondly biological cells onto the SPR sensing area. SPR measurements were then performed by varying the target concentrations. The electrohydrodynamic flow enabled efficient concentration of small objects (3 μm beads, yeasts) onto the SPR sensing area, which resulted in an order of magnitude increased SPR response. Negative dielectrophoresis was also used to concentrate HEK293 cells onto the metal electrodes surrounded by insulating areas, where the SPR response was improved by one order of magnitude.

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MyDEP: A New Computational Tool for Dielectric Modeling of Particles and Cells

Cited by 82 publications

References 13 publications

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

Dielectrophoresis‐assisted creation of cell aggregates under flow conditions using planar electrodes

Performance improvement of plasmonic sensors using a combination of AC electrokinetic effects for (bio)target capture

Dielectrophoretic cell trapping for improved surface plasmon resonance imaging sensing

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