Myelin-like protrusions of giant phospholipid vesicles prepared by electroformation

Kralj‐Iglič, Veronika; Gomišček, Gregor; Majhenc, Janja; Arrigler, Vesna; Svetina, S.

doi:10.1016/s0927-7757(00)00802-5

Cited by 41 publications

(37 citation statements)

References 24 publications

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“…8,9 Although the population of the vesicles is heterogeneous regarding their shape and dynamics, characteristical shapes were observed in cardiolipin-POPC vesicles. These shapes appear as manycompartment structures, compartments being separated by soft fluctuating walls (Figure 4).…”

Section: Discussionmentioning

confidence: 99%

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Tomšiè

Babnik

Lombardo

et al. 2005

J. Chem. Inf. Model.

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The effect of cardiolipin content on the shape and size of giant palmitoyloleylphosphatidylcholine/cardiolipin vesicles was studied. Unilamellar vesicles were prepared in sugar solution by the method of electroformation, from mixtures containing up to 50% weight ratio of cardiolipin. At room temperature the vesicles containing cardiolipin exhibited abrupt changes in the curvature of the vesicle contour indicating regions of phase separation. The deviations from the spherical shape were larger if vesicles were made from mixtures with a higher content of cardiolipin. Numerous vesicles with soft fluctuating walls were observed. The estimated size of the vesicles containing cardiolipin was found to be smaller than the size of pure palmitoyloleylphosphatidylcholine vesicles.

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Section: Discussionmentioning

confidence: 99%

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Tomšiè

Babnik

Lombardo

et al. 2005

J. Chem. Inf. Model.

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“…9) on vesicles, which we interpret as remains of tethers with which the vesicles were attached to the substrate. While the re-sealing of the tether on the spot where it was torn away from the substrate probably occurs promptly, the integration of the residual tubular structure into the spherical mother vesicle using relaxation processes usually takes hours [37]. A theoretical model has been proposed to explain this phenomenon [38].…”

Section: Free Vs Attached Vesiclesmentioning

confidence: 99%

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Peterlin

Arrigler

2008

Colloids and Surfaces B: Biointerfaces

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A recently described technique (Estes and Mayer, Biochim. Biophys. Acta 1712 (2005) 152-160) for the preparation of giant unilamellar vesicles (GUVs) in solutions with high ionic strength is examined. By observing a series of osmotic swellings followed by vesicle bursts upon a micropipette transfer of a single POPC GUV from a sucrose solution into an iso-osmolar glycerol solution, a value for the permeability of POPC membrane for glycerol, P = (2.09±0.82)·10−8 m/s, has been obtained. Based on this result, an alternative mechanism is proposed for the observed exchange of vesicle interior. With modifications, the method of Estes and Mayer is then applied to preparation of flaccid GUVs.

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“…Such shapes are observed in the last stage of spontaneous process in which myelin-like protrusions are integrated into the mother vesicle (Fig. 1); when GPVs are formed by electroformation they are connected by a network of nanotubules (Mathivet et al 1996;Kralj-Iglič et al 2001). Rinsing GPVs from electroformation chamber tears this network, however, its remnants remain attached to GPVs.…”

Section: Introductionmentioning

confidence: 95%

“…In GPVs and in cells, tube-like protrusions are commonly observed. These protrusions may also be undulated thereby forming multiple necks by which the "beads" are connected to form the protrusion (Kralj- Iglič et al 2001;Iglič et al 2007). Such shapes are observed in the last stage of spontaneous process in which myelin-like protrusions are integrated into the mother vesicle (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Due to a spontaneous process the average mean curvature of the GPVs decreases with time (Kralj-Iglič et al 2001;Božič et al 2002). The reason for the transformation is not known, but presumably, phospholipid molecules are slowly removed from the outer membrane layer due to equalization of the chemical potential in the solution and in the membrane, degradation of lipid and lipid flip flop (Kralj-Iglič et al 2001). The protrusion (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Suppression of membrane vesiculation as anticoagulant and anti-metastatic mechanism. Role of stability of narrow necks

Štukelj

Šuštar²,

Mrvar-Brečko³

et al. 2013

gpb

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Abstract. Nanovesicles that are pinched off from biological membranes in the final stage of budding constitute a cell-cell communication system. Recent studies indicate that in vivo they are involved in blood clot formation and in cancer progression. The bud is connected to the mother membrane by a thin neck so it dwells close to the mother membrane. Using the electron microscopy we have observed in blood cells that adhesion between the membrane of the bud and of the mother cell in the vicinity of the neck took place and prevented the bud to pinch off from the mother vesicle. The same effect was observed in giant phospholipid vesicles (GPVs) due to attractive interaction between the bud and the mother vesicle mediated by the plasma protein beta-2-glycoprotein I. The stability of the neck is important for this process. By using Fourier method we analyzed thermal fluctuations of a GPV while a protrusion composed of beads connected by thin necks was spontaneously integrated into the mother GPV. Stepwise change of Fourier coefficients indicates an increased stability of necks which contributes to the retention of buds by the mother membrane and promotes anticoagulant and anti-metastatic mechanism by suppression of nanovesiculation.

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Myelin-like protrusions of giant phospholipid vesicles prepared by electroformation

Cited by 41 publications

References 24 publications

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Electroformation in a flow chamber with solution exchange as a means of preparation of flaccid giant vesicles

Suppression of membrane vesiculation as anticoagulant and anti-metastatic mechanism. Role of stability of narrow necks

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