Myelin sheath formation in cultures of avian spinal ganglia

Effects of ionizing radiations on myelin formation in rat brain cultures

“…Peripheral myelin in vitro is formed by spiral wrapping of Schwann cell cytoplasm around the nerve cell axon (Peterson and Murray, 1955;Peterson et al 1960). Central myelin is formed in ~ by spiral wrapping of the oligodendroglia!…”

Culture of Neural Tissue and Cells in Research Methods in Neurochemistry. Vol. Ii.

“…A method of "patching" the fibrin clot in order to provide a continuous support (Peterson and Murray, 1955) was subsequently developed for neural tissue. This procedure permitted maintenance of explants for several months in culture.…”

Synthesis of Cholesterol and Fatty Acids in Fractions of Peripheral Nerve*†

“…Ben Geren (5) found that the myelin lamellae are formed as a result of winding around the axon layer by layer, the ends remaining attached on the inside to the axolemma and on the outside to the Schwann cell membrane. The Schwann cells have also been observed to deposit a fibrous layer binding them tightly to the nerve axons before the onset of myelination (4). The remainder of the nerve connective tissue is produced from fibroblasts (6).…”

“…Embryological and histological investigations have indicated that both axons and Schwann cells are necessary in the growth and maintenance of the myelin sheaths (2)(3)(4). Ben Geren (5) found that the myelin lamellae are formed as a result of winding around the axon layer by layer, the ends remaining attached on the inside to the axolemma and on the outside to the Schwann cell membrane.…”

“…When myelinization takes place, it occurs simultaneously in several different areas. These are the areas in which Schwann cells are in contact with the nerve (4 Incubation. Control nerves were placed in one flask modified according to Baruch and Chaikoff (10), the homogenate in another, and into a third the sheath was placed.…”