Myelinated axons and functional blood vessels populate mechanically compliant rGO foams in chronic cervical hemisected rats

Domínguez‐Bajo, Ana; González‐Mayorga, Ankor; Guerrero, Carlos R.; Palomares, F. J.; García, Ricardo García; López-Dolado, Elisa; Serrano, María Concepción

doi:10.1016/j.biomaterials.2018.11.024

Cited by 52 publications

(43 citation statements)

References 73 publications

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“…Some mammalian cells show an elastic modulus value below 1 kPa range. 84,85 In general, an eukaryotic cell is about 4 orders the magnitude softer than a single protein (B50 MPa), 24 six orders of magnitude softer than polystyrene (B2 GPa) 86 and about eight orders magnitude softer than silicon (B150 GPa). 87 Under the same applied force (say 300 pN), the best AFM image of a eukaryotic cell has a spatial resolution of about 100 nm, this is, about 3 orders of magnitude worse than the one obtained on a rigid crystalline surface (B0.1 nm).…”

Section: Softness Viscoelasticity Finite-thickness and Incompressibmentioning

confidence: 99%

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Nanomechanical mapping of soft materials with the atomic force microscope: methods, theory and applications

2020

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Section: Softness Viscoelasticity Finite-thickness and Incompressibmentioning

confidence: 99%

“…67,337 The use colloidal tips with radii ranging from 1-50 mm is recommended for generating maps of the mechanical properties of tissues or decellularized extracellular matrices. 85,[338][339][340] In those samples the regions of interest might be several micrometers wide.…”

Section: Quantitative Accuracymentioning

confidence: 99%

Nanomechanical mapping of soft materials with the atomic force microscope: methods, theory and applications

2020

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“…7 Several AFM experiments have shown a correlation between the local stiffness of a cell with its physiological and/or pathological state. 2,[8][9][10][11][12][13][14][15][16][17][18][19] In general, AFM studies are strengthening our understanding of the role of mechanical properties in cell development, differentiation and physiology.…”

Section: Introductionmentioning

confidence: 99%

Nanorheology of living cells measured by AFM-based force–distance curves

2020

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Mechanobiology aims to establish functional relationships between the mechanical state of a living a cell and its physiology. The acquisition of force-distance curves with an AFM is by far the dominant method to characterize the nanomechanical properties of living cells. However, theoretical simulations have shown that the contact mechanics models used to determine the Young's modulus from a force-distance curve could be off by a factor 5 from its expected value. The semi-quantitative character arises from the lack of a theory that integrates the AFM data, a realistic viscoelastic model of a cell and its finitethickness. Here, we develop a method to determine the mechanical response of a cell from a force-distance curve. The method incorporates bottom-effect corrections, a power-law rheology model and the deformation history of the cell. It transforms the experimental data into viscoelastic parameters of the cell as a function of the indentation frequency. The quantitative agreement obtained between the experiments performed on living fibroblast cells and the analytical theory supports the use of force-distance curves to measure the nanorheological properties of cells. † Electronic supplementary information (ESI) available: Complete derivation of the analytical expressions for PLR and KV models, coefficients for a conical tip, correspondence between velocities and equivalent indentation frequencies, statistical analysis of the experiments performed on NIH 3T3 fibroblasts. See

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“…Conversely, PEG and CNF animals were characterized by an interruption of the right fiber tracts. In PEG-treated SCI rats, we observed a partial collapsing of the spinal tissue surrounding the injury, resulting in irregular boundaries ( 25 , 26 ). On the contrary, CNF provided mechanical stability to the lesion site and appeared as a highly hypointense (black) area on MRI images ( Fig.…”

Section: Resultsmentioning

confidence: 95%

Functional rewiring across spinal injuries via biomimetic nanofiber scaffolds

Usmani

Biagioni

Medelin

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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The regrowth of severed axons is fundamental to reestablish motor control after spinal-cord injury (SCI). Ongoing efforts to promote axonal regeneration after SCI have involved multiple strategies that have been only partially successful. Our study introduces an artificial carbon-nanotube based scaffold that, once implanted in SCI rats, improves motor function recovery. Confocal microscopy analysis plus fiber tracking by magnetic resonance imaging and neurotracer labeling of long-distance corticospinal axons suggest that recovery might be partly attributable to successful crossing of the lesion site by regenerating fibers. Since manipulating SCI microenvironment properties, such as mechanical and electrical ones, may promote biological responses, we propose this artificial scaffold as a prototype to exploit the physics governing spinal regenerative plasticity.

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Myelinated axons and functional blood vessels populate mechanically compliant rGO foams in chronic cervical hemisected rats

Cited by 52 publications

References 73 publications

Nanomechanical mapping of soft materials with the atomic force microscope: methods, theory and applications

Nanomechanical mapping of soft materials with the atomic force microscope: methods, theory and applications

Nanorheology of living cells measured by AFM-based force–distance curves

Functional rewiring across spinal injuries via biomimetic nanofiber scaffolds

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