Myelination of regenerating optic nerve axons occurs in conjunction with an increase of the oligodendrocyte precursor cell population in the adult mice

Mendonça, Henrique Rocha; Boas, Camila Oliveira Goulart Villas; Heringer, Luiza dos Santos; Oliveira, Jorge de; Martínez, Ana Maria Blanco

doi:10.1016/j.brainresbull.2020.11.012

Cited by 4 publications

(2 citation statements)

References 55 publications

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“…Furthermore, it would be interesting to investigate in the future whether the changes we described during development are conserved in adulthood and during regeneration. For example, oligodendrocytes expressing sox10 have been described to be involved in mouse regeneration (Mendonça et al., 2021 ). Zebrafish, an animal with continuous growth and where multiple de and remyelination events occur could be an ideal model for this type of research (Chung et al., 2013 ; Fang et al., 2014 ; Zou & Hu, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Oligodendrocyte origin and development in the zebrafish visual system

Santos-Ledo

Pérez-Montes

DeOliveira-Mello

et al. 2022

J of Comparative Neurology

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Oligodendrocytes are the myelinating cells in the central nervous system. In birds and mammals, the oligodendrocyte progenitor cells (OPCs) originate in the preoptic area (POA) of the hypothalamus. However, it remains unclear in other vertebrates such as fish. Thus, we have studied the early progression of OPCs during zebrafish visual morphogenesis from 2 days post fertilization (dpf) until 11 dpf using the olig2:EGFP transgenic line; and we have analyzed the differential expression of transcription factors involved in oligodendrocyte differentiation: Sox2 (using immunohistochemistry) and Sox10 (using the transgenic line sox10:tagRFP). The first OPCs (olig2:EGFP/Sox2) were found at 2 dpf in the POA. From 3 dpf onwards, these olig2:EGFP/Sox2 cells migrate to the optic chiasm, where they invade the optic nerve (ON), extending toward the retina. At 5 dpf, olig2:EGFP/Sox2 cells in the ON also colocalize with sox10:tagRFP. When olig2:EGFP cells differentiate and present more projections, they become positive only for sox10:tagRFP. olig2:EGFP/sox10: tagRFP cells ensheath the ON by 5 dpf when they also become positive for a myelin marker, based on the mbpa:tagRFPt transgenic line. We also found olig2:EGFP cells in other regions of the visual system. In the central retina at 2 dpf, they are positive for Sox2 but later become restricted to the proliferative germinal zone without this marker. In the ventricular areas of the optic tectum, olig2:EGFP cells present Sox2 but arborized ones sox10:tagRFP instead. Our data matches with other models, where OPCs are specified in the POA and migrate to the ON through the optic chiasm.

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Section: Discussionmentioning

confidence: 99%

Oligodendrocyte origin and development in the zebrafish visual system

Santos-Ledo

Pérez-Montes

DeOliveira-Mello

et al. 2022

J of Comparative Neurology

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“…Aberrant white matter development leads to pathologies such as leukodystrophies and white matter injury of prematurity (Bugiani et al, 2013;Back and Rosenberg, 2014;Elitt and Rosenberg, 2014;Volpe, 2019;Garcia et al, 2020). In adults, endogenous oligodendrocyte progenitors can be mobilized for myelin repair following injury, such as in multiple sclerosis (Chang et al, 2002;Back et al, 2005), white matter stroke (Sozmen et al, 2016;Sozmen et al, 2019) and trauma (Rodriguez et al, 2014;Mendonca et al, 2021). A common theme in these pediatric and adult white matter disorders is oligodendrocyte differentiation arrest (Back et al, 2005;Nakahara et al, 2009;Bugiani et al, 2013;Cree et al, 2018;Srivastava et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Developmental regulation of zinc homeostasis in differentiating oligodendrocytes

Elitt

Ross

Wang

et al. 2023

Preprint

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Oligodendrocytes develop through well characterized stages and understanding pathways regulating their differentiation remains an active area of investigation. Zinc is required for the function of many enzymes, proteins and transcription factors, including those important in myelination and mitosis. Our previous studies using the ratiometric zinc sensor chromis-1 demonstrated a reduction in intracellular free zinc concentrations in mature oligodendrocytes compared with earlier stages (Bourassa et al., 2018). We performed a more detailed developmental study to better understand the temporal course of zinc homeostasis across the oligodendrocyte lineage. Using chromis-1, we found a transient increase in free zinc after developing oligodendrocytes were switched into differentiation medium. To gather other evidence for dynamic regulation of free zinc during oligodendrocyte development, qPCR was used to evaluate mRNA expression of the major zinc storage proteins metallothioneins (MTs), and metal regulatory transcription factor 1 (MTF-1) which controls expression of MTs. MT-1, MT-2 and MTF1 mRNAs were all increased several fold in mature oligodendrocytes compared to developing oligodendrocytes. To assess the depth of the zinc buffer, we assayed zinc release from intracellular stores using the oxidizing thiol reagent 2,2'-dithiodipyridine (DTDP). Exposure to DTDP resulted in a ~100% increase in free zinc in developing oligodendrocytes but, paradoxically more modest ~60% increase in mature oligodendrocytes despite the increased expression of MTs. These results suggest that zinc homeostasis is regulated during oligodendrocyte development, that oligodendrocytes are a useful model for studying zinc homeostasis in the central nervous system, and that regulation of zinc homeostasis may be important in oligodendrocyte differentiation.

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