Myelosuppressive activity of two herbicides, atrazine and dinoterb, on human haematopoietic progenitor cells: An in vitro assay to evaluate the effects of intermediate or long-term exposure

Sawicki, B; Durand, Geneviève; Dewitte, J.-D.; Ratanasavanh, Damrong; Riche, C.; Leglise, Marie-Chantal

doi:10.1016/s0887-2333(97)00109-4

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…had better protective effects on TNF‐α than in animals co‐treated with curcumin and quercetin separately at the same tested doses, suggesting the fact that the combined effects of both compounds could better maintain the inflammatory network of the testes required for normal testicular functions. The decrease in myeloperoxidase activity in the present study further confirms the immunosuppressive effect of ATZ in experimental models (Abarikwu, Oleribe, et al, 2020; Chen et al, 2014; Sawicki et al, 1998; Zhao et al, 2013). The animals that were co‐treated with curcumin at high dose (100 mg/kg b.w.)…”

Section: Discussionsupporting

confidence: 86%

Curcumin improves the protective effects of quercetin against atrazine‐induced testicular injury in adult Wistar rats

2022

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This study evaluated the beneficial protective effect of cotreatment of curcumin (CUR) and quercetin (QUE) on atrazine (ATZ)‐induced testicular toxicity in rats. ATZ challenge diminished luteinizing hormone, follicular stimulating hormone, testosterone and myeloperoxidase enzyme activity, but these effects were attenuated on co‐treatment with CUR and QUE. Also, co‐treatment of CUR + QUE was better than separate administration of QUE at diminishing malondialdehyde and glutathione and improving tumour necrosis factor‐α concentration, germ cell numbers (spermatogonia, spermatocytes and round spermatids) and epididymal sperm quality. Histologically, smaller sized tubules with degenerated epithelia and few germ cells were seen in the seminiferous tubules of the ATZ group whereas CUR + QUE pretreatment improved the histo‐morphologic features of the tubules compared to the ATZ group and was also better than separate administration of QUE. We conclude that CUR can improve the protective effects of QUE against ATZ‐induced testicular injury by enhancing the levels of reproductive hormones, recovering testicular biochemical parameters and improving the histological features of the testes.

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Section: Discussionsupporting

confidence: 86%

Curcumin improves the protective effects of quercetin against atrazine‐induced testicular injury in adult Wistar rats

2022

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“…For the triazines treated mice or those co-treated with LPS, a sparse mass inflammatory cells could be seen in some dilated hepatic sinusoids and around the central vein, suggesting that the anti-inflammatory effects of triazines were responsible for the decreased aggregate mass of inflammatory cells around the central vein. The fact that ATZ decreases the level of the anti-inflammatory cytokine, IL-4 in mice splenic cells and induces myelosuppressive effects in the hepatoma cell lines and liver [ 11 , 57 ] further support the immunosuppressive and anti-inflammatory effects of triazines in mammalian model systems [ 2 , 8 ]. The present findings also support the fact that LPS causes tissue injury by mechanisms involving chemical mediators such as nitric oxide, TNF-α and other cytokines [ 58 , 59 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of triazines and lipopolysaccharide coexposure on inflammatory response and histopathological changes in the testis and liver of BalB/c mice

Abarikwu,

Mgbudom-Okah,

Ndufeiya-Kumasi

et al. 2024

Heliyon

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“…Manske et al (2004) showed that low-level, short-term ATR exposure to normal fibroblasts results in decreased cell proliferation. Moderate cytotoxic effect of ATR (IC 50 37.4 μM) and its metabolite 2-chloro-diamino-atrazine (IC 50 37.0 μM) on normal human granulo-monocytic progenitor cells (CFU-GM) was found by Sawicki et al (1998). Kmetic et al (2008) confirmed that ATR decreases hamster ovarian cell (CHO-K1) proliferation.…”

mentioning

confidence: 86%

“…Likewise, most investigations confirmed noncytotoxic effect of high concentration of ATR and its metabolites on cell proliferation in vitro. Atrazine was found to be non-toxic to human liver carcinoma HepG 2 cells (Thounwou et al 2001;Jondeau et al 2006) and rat primary hepatocytes (Sawicki et al 1998). No cytotoxic effect was recorded with ATR at concentration of up to 100 mM on sheep peripheral blood phagocytes and lymphocytes under in vitro conditions.…”

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confidence: 92%

In Vitro Studies on Atrazine Effects on Human Intestinal Cells

Olejnik

Marecik

Białas

et al. 2010

Water Air Soil Pollut

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Considering the importance of the oral route for human exposure to atrazine, we have investigated the possible effect of this herbicide on the human intestinal cells and the integrity of the epithelial barrier, using Caco-2 cells as the intestinal model in vitro. We evaluated possibile cytotoxic and genotoxic effects of atrazine in concentrations ranging from 1 to 250 μM on the Caco-2 cells at different stages of growth after short-and long-term exposure. Results from the tetrazolium blue (MTT) test and the Trypan blue exclusion assay showed that atrazine cytotoxicity was dose-and time-dependent. Obtained data indicated that atrazine at high concentrations (50 and 250 μM) was able to induce effects on Caco-2 proliferation and viability. Moreover, it was found that the long-term exposure to atrazine at the noncytotoxic dose caused inhibition of the intestinal cell maturation and decreased the transepithelial electrical resistance, the indicator of the epithelial barrier integrity. Studies on the atrazine genotoxicity determined using the single cell microelectrophoresis assay indicated that atrazine did not induce DNA damages in the Caco-2 cells at concentrations of up to 50 μM, whereas enhancement in the DNA damage was observed at 250 μM. Altogether, our results indicate that atrazine at expected human oral exposure concentrations is not able to induce effects on the Caco-2 cell proliferation and viability, but may suppress the intestinal cell differentiation and reduce the cell monolayer integrity. We suggest that chronic exposure on low levels of atrazine may lead to alteration in the expression of the morphological and functional features of the Caco-2 cells related to the transport and barrier function of small intestinal enterocytes. In consequence, this may lead to alterations in the intestinal absorption process.

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Myelosuppressive activity of two herbicides, atrazine and dinoterb, on human haematopoietic progenitor cells: An in vitro assay to evaluate the effects of intermediate or long-term exposure

Cited by 9 publications

References 21 publications

Curcumin improves the protective effects of quercetin against atrazine‐induced testicular injury in adult Wistar rats

Curcumin improves the protective effects of quercetin against atrazine‐induced testicular injury in adult Wistar rats

Influence of triazines and lipopolysaccharide coexposure on inflammatory response and histopathological changes in the testis and liver of BalB/c mice

In Vitro Studies on Atrazine Effects on Human Intestinal Cells

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