Myo-cytoblots: Quantification of dystrophin by in-cell western assay for a streamlined development of DMD treatments

Ruiz-del-Yerro, Estibaliz; Garcia-Jimenez, Iker; Arechavala‐Gomeza, Virginia

doi:10.1016/j.nmd.2016.06.267

Cited by 1 publication

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“…It is an extremely sensitive method and accurately quantifies, which can detect two targets normally labeled with specific primary antibody followed by incubation with secondary antibody fluorescent conjugated with spectrally distinct dyes and quantification of the signals from fluorophores conjugated at different wavelengths on two detection channels. The technique allowed the quantification of proteins directly in cell culture [28]. The accuracy of quantification is increased by normalization due to adjustments of the cell number in wells.…”

Section: In-cell Western (Icw) Assaymentioning

confidence: 99%

Current Approaches in Immunoassay Methods Focus on Skeletal Muscle Proteins

Găină¹

2020

Muscle Cells - Recent Advances and Future Perspectives

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The skeletal muscle is a complex tissue that represents most of the muscle tissue in mammals and plays a key role in health and in the body's function. It is a heterogeneous tissue whose contractile and metabolic functions depend on type, size, and quality of a large number of proteins. The multitude of proteins, the relationships that exist between them, and functional changes that occur in different muscle pathologies make their investigation to be challenged. In this chapter, current approaches in proteomic studies, its application, specific technical advice, and recent progress of the most important techniques based on antigen-antibody interactions used for the analysis of muscle proteins involved in different muscle diseases are presented.

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Section: In-cell Western (Icw) Assaymentioning

confidence: 99%

Current Approaches in Immunoassay Methods Focus on Skeletal Muscle Proteins

Găină¹

2020

Muscle Cells - Recent Advances and Future Perspectives

View full text Add to dashboard Cite

show abstract

Myo-cytoblots: Quantification of dystrophin by in-cell western assay for a streamlined development of DMD treatments

Cited by 1 publication

References 0 publications

Current Approaches in Immunoassay Methods Focus on Skeletal Muscle Proteins

Current Approaches in Immunoassay Methods Focus on Skeletal Muscle Proteins

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