Myo-Inositol Oxygenase as a Novel Marker in the Diagnosis of Acute Kidney Injury

Mertoğlu, Cuma; Günay, Murat; Gürel, Ali; Güngör, Mehmet Ali̇

doi:10.1515/jomb-2017-0027

Cited by 15 publications

(13 citation statements)

References 23 publications

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“…Furthermore, the specifi city of the MIOX enzyme to the kidneys was demonstrated by western blotting technique in that study. In another recent study on human subjects from our research group, serum MIOX level was detected to be higher in the patient group with increased serum creatinine at the time of diagnosis of AKI than in the healthy control group (10). In another study, in which we investigated the effect of retrograde intrarenal surgery on kidney tissue in renal stone treatment, the serum MIOX level was found to be benefi cial in showing renal damage.…”

Section: Discussionmentioning

confidence: 51%

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Effectiveness of myo-inositol oxygenase in the early diagnosis of experimental acute kidney injury

Mertoğlu¹,

Özmen²,

Fırat³

et al. 2020

BLL

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OBJECTIVES: The effectiveness of the myo-inositol oxygenase (MIOX) enzyme was investigated in the diagnosis of acute kidney injury (AKI). METHODS: In total, 40 rats were divided into 5 groups (n = 8, for each group) while left kidney ischemiareperfusion was implemented in groups 2, 3, 4 and 5. Group 1 was the control group. Group 2 underwent 1-hour ischemia and 2-hour reperfusion. Group 3 underwent 1-hour ischemia and 4-hour reperfusion. Group 4 underwent 2-hour ischemia and 2-hour reperfusion. Group 5 underwent 2-hour ischemia and 4-hour reperfusion. RESULTS: Serum creatinine and blood urea nitrogen levels in all ischemia-reperfusion groups were higher than in the control group (p < 0.001). Serum MIOX level was higher in groups 2, 3 and 4 than in group 1 (p =0.002). Tissue MIOX level was lower in groups 2, 4, and 5 than in group 1 (p = 0.039). Serum and tissue neutrophil gelatinase-associated lipocalin levels were not signifi cantly different between the groups. The injury level in histopathologic examination was as follows: group 1 < group 3 < group 2 = group 4 < group 5. CONCLUSION: The serum MIOX level increases in the early stages of AKI, however, decreases subsequently. Therefore, the serum MIOX may be a potential promising biomarker in the early diagnosis of AKI (Tab. 5, Fig. 6, Ref. 30).

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Section: Discussionmentioning

confidence: 51%

“…This is a unique pathway for MI catabolism (7)(8)(9). Although the MIOX enzyme is one of rare organ-specifi c enzymes, particularly a renal-specifi c enzyme, the data on the role of MIOX in the diagnosis of AKI are limited (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of myo-inositol oxygenase in the early diagnosis of experimental acute kidney injury

Mertoğlu¹,

Özmen²,

Fırat³

et al. 2020

BLL

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“…In a previous study, the diagnostic sensitivity and specificity of the MIOX molecule released from the proximal tubules of kidneys in showing acute kidney injury were observed as 53.8% and 81.5%, respectively. Accordingly, it was concluded that MIOX could be used as a marker of acute kidney injury [21]. In addition, when compared to creatinine, MIOX was observed to increase much earlier in case of kidney injury [21].However, a review of the literature shows no study comparing the MIOX molecule in patients that have undergone PNL and RIRS.…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, it was concluded that MIOX could be used as a marker of acute kidney injury [21]. In addition, when compared to creatinine, MIOX was observed to increase much earlier in case of kidney injury [21].However, a review of the literature shows no study comparing the MIOX molecule in patients that have undergone PNL and RIRS.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Serum Kim-1 and Miox Levels in Patients That Underwent Percutaneous Nephrolithotomy and Flexible Ureterorenoscopy

Altun¹,

Bozkurt²,

Erdoğan³

et al. 2021

Preprint

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Aim: Percutaneous nephrolithotomy (PNL) and retrograde intrarenal surgery (RIRS) are common surgical methods in the treatment of kidney stones. Possible effects on kidneys are an important factor in determining the surgical procedure and the surgical method. In our study, kidney injury molecule-1 (KIM-1) and myo-inositol oxygenase (MIOX) were used to compare acute kidney injury in patients that underwent PNL and RIRS. Material and Method:Eighty patients aged 20 to 75, who underwent PNL or RIRS in our urology clinic between November 2018 and February 2020 were included in the study. In this prospective study, the demographic characteristics, stone size, operation time, preoperative and postoperative hemoglobin and biochemistry values of the patients were recorded. 5 cc blood samples taken from the patients before the operation and at the fourth hour after the operation were centrifuged and kept at -80 °C, and the KIM-1 and MIOX levels were measured in the biochemistry department. Results: There was no difference between the groups in terms of demographic data; however,the operation time and length of hospital stay were significantly longer in the PNL group. The mean increase in MIOX was 10.583±9.73 ng/ml and 7.501±16.46 ng/ml in the PNL and RIRS groups, respectively. Although there was a statistically significant increase in both groups, this increase was greater in the PNL group. A significant increase was observed only in the PNL group in the postoperative period (p=0.003). Discussion and Conclusion:The findings of the study suggest that the PNL procedure causes more damage to the kidneys than RIRS.

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“…Recent studies further found that Miox is a kidney-specific proximal tubule protein that increased in the serum of animal with AKI or diabetic nephropathy and the plasma of patients with severe AKI patients or type 2 diabetes mellitus 25 – 27 . In addition, cadmium-induced toxicity in mice kidney significantly increased Miox levels in the tubular cells 28 , and the Miox concentration in the serum and urine may negatively reflect the rental function 29 . Since Miox is a highly conserved enzyme in mouse, rat, and human kidney, Miox is likely to be an ideal biomarker of AKI for early detection of kidney injury.…”

Section: Introductionmentioning

confidence: 99%

Kidney-based in vivo model for drug-induced nephrotoxicity testing

Chiou

Jiang

Ding

et al. 2020

Sci Rep

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The need is critical and urgent for a real-time, highly specific, and sensitive acute kidney injury biomarker. This study sought to establish a sensitive and specific Miox-NanoLuc transgenic mouse for early detection of drug-induced nephrotoxicity. We generated Miox-NanoLuc transgenic mice with kidney-specific NanoLuc overexpression. Our data showed that Miox-NanoLuc-produced luminescence was kidney-specific and had good stability at room temperature, 4 °C, − 20 °C, and repeated freeze–thaw cycles. Serum levels of BUN and creatinine were significantly increased at day 2 or 3 in cisplatin-treated mice and at day 5 in aristolochic acid (AAI)-treated mice. Particularly, the serum and urine Miox-NanoLuc luminescence levels were significantly increased at day 1 in cisplatin-treated mice and at day 3 in AAI-treated mice. Renal pathological analysis showed that the kidney sections of cisplatin-treated mice at day 5 and AAI-treated mice at day 13 showed cytolysis and marked vacuolization of tubular cells. In conclusion, we developed a new platform to early quantify drug-induced nephrotoxicity before serum BUN and creatinine levels increased and pathological tubular cell injury occurred. This model may serve as an early detection for drug- and food-induced nephrotoxicity and as an animal model to investigate tubular cell injury.

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Myo-Inositol Oxygenase as a Novel Marker in the Diagnosis of Acute Kidney Injury

Cited by 15 publications

References 23 publications

Effectiveness of myo-inositol oxygenase in the early diagnosis of experimental acute kidney injury

Effectiveness of myo-inositol oxygenase in the early diagnosis of experimental acute kidney injury

Comparison of Serum Kim-1 and Miox Levels in Patients That Underwent Percutaneous Nephrolithotomy and Flexible Ureterorenoscopy

Kidney-based in vivo model for drug-induced nephrotoxicity testing

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