Myo1g is an active player in maintaining cell stiffness in B‐lymphocytes

López‐Ortega, Orestes; Ovalle-García, Erasmo; Ortega‐Blake, Iván; Antillón, Armando; Chávez‐Munguía, Bibiana; Patiño-López, Genaro; Fragoso‐Soriano, Rogelio; Santos‐Argumedo, Leopoldo

doi:10.1002/cm.21299

Cited by 26 publications

(28 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A more detailed follow-up on the relevance of these fusions will be necessary, as our data on orientation and on in frame fusions of the coding sequences (CDS) gives only a first perception on potential functionality. Based on this limited data, PAX5-MYO1G, PAX5-NCOA6, and possibly PAX5-LINC01251 seem to be promising new fusions, whose influence on the course of the disease warrants further consideration [34,35]. Just recently, the clinical implication of chimeric in frame PAX5 fusions has been highlighted, as well as the potentially initiating, subtype-defining character of PAX5 alterations in B-ALL [33].…”

Section: Discussionmentioning

confidence: 99%

Implementation of RNA sequencing and array CGH in the diagnostic workflow of the AIEOP-BFM ALL 2017 trial on acute lymphoblastic leukemia

et al. 2020

View full text Add to dashboard Cite

Risk-adapted therapy has significantly contributed to improved survival rates in pediatric acute lymphoblastic leukemia (ALL) and reliable detection of chromosomal aberrations is mandatory for risk group stratification. This study evaluated the applicability of panel-based RNA sequencing and array CGH within the diagnostic workflow of the German study group of the international AIEOP-BFM ALL 2017 trial. In a consecutive cohort of 117 children with B cell precursor (BCP) ALL, array analysis identified twelve cases with an IKZF1 plus profile of gene deletions and one case of masked hypodiploidy. Genetic markers BCR-ABL1 (n = 1), ETV6-RUNX1 (n = 25), and rearrangements involving KMT2A (n = 3) or TCF3 (n = 3) were assessed by established conventional techniques such as karyotyping, FISH, and RT-PCR. Comparison of these results with RNA sequencing analysis revealed overall consistency in n=115/117 cases, albeit with one undetected AFF1-KMT2A fusion in RNA sequencing and one undetected ETV6-RUNX1 fusion in conventional analyses. The combined application of RNA sequencing, FISH, and CGH+SNP array reliably detected all genetic markers necessary for risk stratification and will be used as the diagnostic standard workflow for BCP-ALL patients enrolled in the AIEOP-BFM ALL 2017 study. Prospectively, consistent collection of genome-wide CGH+SNP array as well as RNA sequencing data will be a valuable source to elucidate new prognostic lesions beyond established markers of pediatric ALL. In this respect, RNA sequencing identified various gene fusions in up to half of the IKZF1 plus (n = 6/12) and Bother (n = 19/36) cases but not in cases with hyperdiploid karyotypes (n = 35). Among these fusions, this study reports several previously undescribed in frame PAX5 fusions, including PAX5-MYO1G and PAX5-NCOA6.

show abstract

Section: Discussionmentioning

confidence: 99%

Implementation of RNA sequencing and array CGH in the diagnostic workflow of the AIEOP-BFM ALL 2017 trial on acute lymphoblastic leukemia

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The meshwork is approximately 100 nm thick, has a pore size of about 50 nm and attaches to the plasma membrane via ezrin, moesin and class I myosins [15][16][17] . Actin polymerization at the plasma membrane is constantly balanced by actin destruction at the cytosolic side of the cortex by actin severing proteins, cofilin and destrin 14,18,19 , and by the debranching activity of coronins 20 .…”

Section: [H1] Structure Of the Cortical Cytoskeletonmentioning

confidence: 99%

Cytoskeletal control of B cell responses to antigens

Tolar

2017

Nat Rev Immunol

125

View full text Add to dashboard Cite

The actin cytoskeleton is essential for cell mechanics and has increasingly been implicated in the regulation of cell signalling. In B cells, the actin cytoskeleton couples extensively to B cell receptor (BCR) signalling pathways and its defects can either enhance or suppress B cell activation. Recent insights from single-cell imaging and biophysical techniques suggest that actin orchestrates BCR signalling at the plasma membrane through effects on protein diffusion, and that it regulates antigen discrimination through the biomechanics of immune synapses. These mechanical functions also play a role in the adaptation of B cell subsets to specialized tasks during antibody responses.3

show abstract

“…Class I myosins have been involved in the regulation of adhesion, motility and the recycling of receptors through the transport of vesicles and by the interaction with different cytoskeletal proteins (Piedra-Quintero et al, 2019, López-Ortega and Santos-Argumedo, 2017, Maravillas-Montero et al, 2011). However, few works are analyzing how class I myosins participate in the signaling mechanism of in leukocytes during cell migration (Salvermoser et al, 2018, López-Ortega et al, 2016). In the present study, through intravital microscopy, we demonstrated the relevance of the Myo1e in the motility of activated B cells.…”

Section: Discussionmentioning

confidence: 99%

Myo1e modulates the recruitment of B cells to inguinal lymph nodes

Girón-Pérez

Santos-Argumedo

Vadillo

et al. 2019

Preprint

View full text Add to dashboard Cite

The recruitment of leukocyte to high endothelium venules and their migration to the lymph nodes are critical steps to initiate an immune response. Cell migration is regulated by the actin cytoskeleton where myosins have a very import role. Myo1e is a long tail class I myosin highly expressed in B cells that not have been studied in the context of cell migration. By using an in vivo model, through the use of intravital microscopy, we demonstrated the relevance of Myo1e in the adhesion and the migration of B cells in high endothelial venules. These observations were confirmed by in vitro experiments. We also registered a reduction in the expression of integrins and F-actin in the protrusion of B lymphocytes membrane. Deficiencies in vesicular trafficking can explain the decrease of integrins on the surface. Interestingly, Myo1e is associated with focal adhesion kinase (FAK). The lack of Myo1e affected the phosphorylation of FAK and AKT, and the activity of RAC-1, disturbing the FAK/PI3K/RAC-1 signaling pathway. Together, our results indicate critical participation of Myo1e in the mechanism of B cell migration.Summary statementMyo1e participate in the adhesion and migration in the high endothelial venules by regulation of integrins and the PI3K/FAK/RAC-1 signaling pathway.

show abstract

Myo1g is an active player in maintaining cell stiffness in B‐lymphocytes

Cited by 26 publications

References 40 publications

Implementation of RNA sequencing and array CGH in the diagnostic workflow of the AIEOP-BFM ALL 2017 trial on acute lymphoblastic leukemia

Implementation of RNA sequencing and array CGH in the diagnostic workflow of the AIEOP-BFM ALL 2017 trial on acute lymphoblastic leukemia

Cytoskeletal control of B cell responses to antigens

Myo1e modulates the recruitment of B cells to inguinal lymph nodes

Contact Info

Product

Resources

About