Myoblast mechanotransduction and myotube morphology is dependent on BAG3 regulation of YAP and TAZ

Günay, Kemal Arda; Chang, Tze-Ling; Bednarski, Olivia J.; Bannister, Kendra L.; Rogowski, Cameron J.; Olwin, Bradley B.; Anseth, Kristi S.

doi:10.1016/j.biomaterials.2021.121097

Cited by 20 publications

(8 citation statements)

References 55 publications

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Section: Resultsmentioning

confidence: 99%

“…[38,42] Our results suggest that Ca 2+ release from bioglass nanoparticles can be used to provide mechanical cues for mechanosensitive cells, such as myoblasts, to promote their differentiation into myotubes. [43][44][45][46] Of note, we also conducted experiments analogous to those with added bioglass but instead using silica nanoparticles at the same concentrations. The silica nanoparticles have a similar size and morphology to those of the bioglass particles that we used in our experiments.…”

Section: Production Of Axial Gradientsmentioning

confidence: 99%

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Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Ceballos‐González,

Bolívar‐Monsalve,

Quevedo‐Moreno

et al. 2023

Adv Materials Technologies

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Nature abounds with micro‐architected materials containing layered multi‐material patterns that often transition within the very same monolithic piece. Fabricating these complex materials using current technologies is challenging. Multimaterial chaotic printing is presented—an extrusive printing method based on the use of chaotic advection—that can fabricate microstructured hydrogels with well‐defined multimaterial and multilayered micropatterns. Printheads containing internal Kenics static mixing (KSM) elements and top‐ and lateral‐positioned inlets are used to produce a wide repertoire of multilayered hydrogel filaments. In this plug‐and‐play system, the radial and axial micropatterns can be designed ad hoc by defining the printhead configuration (i.e., the number of KSM elements and inlets, and the inlet positions) and the flow program (i.e., activation/deactivation of the ink‐flow through each inlet). Computational fluid dynamics simulations closely predict the microstructure obtained by a given printhead configuration. The application of this platform is illustrated for easy fabrication of fibers with radial microgradients, bacterial ecosystems, structured emulsions, micro‐channeled hydrogel filaments, a pre‐vascularized tumor niche model, and skeletal muscle‐like tissues with axial and radial transitions of bioactive glass compartments. It is envisioned that multimaterial chaotic printing will be a valuable addition to the toolbox of additive manufacturing for the rational fabrication of advanced materials.

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Section: Resultsmentioning

confidence: 99%

Section: Production Of Axial Gradientsmentioning

confidence: 99%

Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Ceballos‐González,

Bolívar‐Monsalve,

Quevedo‐Moreno

et al. 2023

Adv Materials Technologies

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“…31 The YAP nuclei/cytoplasm ratio was almost the same on both aligned and random fibers as the two types of fibers were rigid enough (Figure S1), provided that intracellular forces excluded the threshold for YAP nuclei entry, which would not affect the myogenesis apart from surface topography. 32 3.4. Aligned Nanofiber Activates Rac-Related Adhesion.…”

Section: Fibrous Structure Affects Cell Mechanosensingmentioning

confidence: 99%

Aligned Nanofibers Promote Myoblast Polarization and Myogenesis through Activating Rac-Related Signaling Pathways

Dong,

Su,

Sun

et al. 2024

ACS Biomater. Sci. Eng.

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The extracellular matrix (ECM) plays a crucial role in regulating cellular behaviors and functions. However, the impact of ECM topography on muscle cell adhesion and differentiation remains poorly understood from a mechanosensing perspective. In this study, we fabricated aligned and random electrospun polycaprolactone (PCL) nanofibers to mimic the structural characteristics of ECM. Mechanism investigations revealed that the orientation of nanofibers promoted C2C12 polarization and myogenesis through Rac-related signaling pathways. Conversely, cells cultured on random fibers exhibited spreading behavior mediated by RhoA/ROCK pathways, resulting in enhanced stress fiber formation but reduced capacity for myogenic differentiation. Our findings highlight the critical role of an ECM structure in muscle regeneration and damage repair, providing novel insights into mechanosensing mechanisms underlying muscle injury diseases.

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“…11,12 Actually, hydrogels are a polymer network with high water content. Both natural polymers, such as hyaluronic acid, 13 gelatin, 14 chitosan, 15 and cellulose 16 and synthetic polymers, such as poly(ethylene glycol), 17 poly(vinyl alcohol), 18 and polyurethane, 19 are widely used in the development of hydrogel-based scaffolds. 20−22 In comparison, synthetic polymer hydrogels are more controllable and generally show higher and more osteoconductive mechanical strength than natural polymer hydrogels.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Hydrogels, due to their flexibility, designability, and multifunctionality, are emerging as a kind of scaffold materials. , Actually, hydrogels are a polymer network with high water content. Both natural polymers, such as hyaluronic acid, gelatin, chitosan, and cellulose and synthetic polymers, such as poly(ethylene glycol), poly(vinyl alcohol), and polyurethane, are widely used in the development of hydrogel-based scaffolds. − In comparison, synthetic polymer hydrogels are more controllable and generally show higher and more osteoconductive mechanical strength than natural polymer hydrogels. , For example, Gong and co-workers developed a series of synthetic polymer hydrogels with stiffness even reaching the MPa level . Among the preparation methods of synthetic polymer hydrogels, free radical polymerization occupies a vital position.…”

Section: Introductionmentioning

confidence: 99%

Tough, Flexible, and Bioactive Amphoteric Copolymer-Based Hydrogel for Bone Regeneration without Encapsulation of Seed Cells/Simulating Cues

Wang

Che

Qian

et al. 2022

ACS Appl. Mater. Interfaces

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Bone tissue scaffolds with good bulk or surface osteoconductivity are always pursued by biomaterial scientists. In this paper, we design a tough and flexible amphoteric copolymerbased (AC) hydrogel with bioactive groups for bone regeneration. In detail, our hydrogels are copolymerized with N-acyl glycinamide (NAGA), anionic acrylate alendronate (AcAln), and cationic (2-(acryloyloxy)ethyl) trimethyl ammonium chloride (DMAEA-Q) by free radical polymerization. There are three kinds of synergetic physical cross-links among our polyamphion hydrogels: (1) double hydrogen bonds between amide groups in NAGA to provide toughness, (2) hydrogen bonds between dual bisphosphite groups in AcAln, and (3) weak ionic pairs between the anionic bisphosphite groups and the cationic quaternary ammonium groups in DMAEA-Q to offer flexibility. The AC hydrogel shows osteoid-like viscoelasticity, which makes the AC hydrogel osteogenesis inductive. During the repairing process, the bioactive bisphosphite groups accelerate the calcium fixation to expedite the mineralization of the new-formed bone. At the same time, the surface charge property of AC hydrogels also prevents fibrous cyst formation, thus guaranteeing osseointegration. Our in vitro data strongly demonstrate that the AC hydrogel is an excellent matrix to induce osteogenesis of rat bone marrow mesenchymal stem cells. More importantly, the following in vivo experiments further prove that the AC hydrogel can reach satisfactory bone regeneration without encapsulation of seed cells or application of external simulating cues. These exciting results demonstrate that our AC hydrogel is a promising scaffold for bone regeneration. Our work can also inspire the constituent and structure design of biomaterial scaffolds for tissue regeneration.

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Myoblast mechanotransduction and myotube morphology is dependent on BAG3 regulation of YAP and TAZ

Cited by 20 publications

References 55 publications

Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Plug‐and‐Play Multimaterial Chaotic Printing/Bioprinting to Produce Radial and Axial Micropatterns in Hydrogel Filaments

Aligned Nanofibers Promote Myoblast Polarization and Myogenesis through Activating Rac-Related Signaling Pathways

Tough, Flexible, and Bioactive Amphoteric Copolymer-Based Hydrogel for Bone Regeneration without Encapsulation of Seed Cells/Simulating Cues

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