Myocardial and Other Muscle Cell Cultures

Halle, W; Wollenberger, A

doi:10.1007/978-1-4615-7425-5_7

Cited by 15 publications

(4 citation statements)

References 132 publications

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“…The cultured cells can be maintained in various experimental media to attempt to change the composition of the cell membranes, and ascertain the concomitant changes in membrane transport and electrical properties. Cultured heart cells can be grown in chemically-defined media (13), and antibiotics may be omitted from the culture medium if careful sterile procedures are followed. Finally, the effects of prolonged exposure (e.g., for several weeks) to various toxicological agents and drugs (at various concentrations) on the myocardial cells can be determined.…”

Section: Advantages Of Using Cultured Heart Cellsmentioning

confidence: 99%

Cultured Heart Cell Reaggregate Model for Studying Cardiac Toxicology

Sperelakis¹

1978

Environmental Health Perspectives

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The National Institute of Environmental Health Sciences (NIEHS) andBrogan & Partners are collaborating with JSTOR to digitize, preserve and extend access to Environmental Health Perspectives. This review represents a summary of the technique of using cultured heart cells as a model system for studying the physiology, pharmacology, biochemistry and toxicology of myocardial cells. The general techniques and types of culture preparations commonly used are given and some of the advantages and disadvantages of working with cultured heart cells are summarized.

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Section: Advantages Of Using Cultured Heart Cellsmentioning

confidence: 99%

Cultured Heart Cell Reaggregate Model for Studying Cardiac Toxicology

Sperelakis¹

1978

Environmental Health Perspectives

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“…13F). Hl 1°0 mm Sr I9 mM Sr ++ _rr~~~~~~~~~~~ WCWlw FIGURE 13. Effects of Ba2+ and Sr2+ on reverted cells in monolayer culture.…”

Section: Pk a Plot Of Resting Potential Versus Log [K]omentioning

confidence: 99%

Cultured heart cell reaggregate model for studying cardiac toxicology.

Sperelakis¹

1978

Environ Health Perspect

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“…When embryonic heart tissue is dissociated with proteolytic enzymes into single cells and then grown in primary culture, a substantial fraction of the cells display spontaneous and rhythmic contractions (1)(2)(3)(4). The time required for the onset of autorhythmicity of primary heart cell cultures is related to the age of the embryo used as the source of the dissociated heart cells (1,5). A second property of the cultured myocyte system is that the cells of auricular origin contract faster than ventricular cells (1,6).…”

mentioning

confidence: 99%

Relationship between dibutyryl cyclic AMP and microtubule organization in contracting heart muscle cells.

Nath¹,

Shay²,

Bollon³

1978

Proc. Natl. Acad. Sci. U.S.A.

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Rhythmic and synchronous contractions of interconnecting myocyte cultures prepared from fetal rat hearts are arrested upon the addition of 0.2-2.0 mM N6,02'-dibutyryladenosine 3':5'-cyclic monophosphate (Bt2cAMP). The contractions arrested by Bt2cAMP are restored either by diluting the Bt2cAMP from the media or by adding colchicine. While colchicine restores Bt2cAMP-arrested myocyte contractions at concentrations as low as 1.0 jIM, the inactive isomer lumicolchicine shows no effect. Morphologically, Bt2cAMP treatment of myocyte cultures results in the appearance of numerous elongated cellular processes not present in control cultures. Ultrastructural examination indicates that in Bt2cAMP-treated cells the intracellular distribution of microtubules is altered such that these organelles appear to accumulate in parallel arrays. In cells not treated with Bt2cAMP, the microtubules appear randomly oriented, while in cells treated with only colchicine, intact microtubules are not observed. The relationship between microtubules and heart cell contraction is discussed. When embryonic heart tissue is dissociated with proteolytic enzymes into single cells and then grown in primary culture, a substantial fraction of the cells display spontaneous and rhythmic contractions (1-4). The time required for the onset of autorhythmicity of primary heart cell cultures is related to the age of the embryo used as the source of the dissociated heart cells (1, 5). A second property of the cultured myocyte system is that the cells of auricular origin contract faster than ventricular cells (1, 6). Thus, the specificity of the compartments of whole heart also manifests itself through the individual cells. Other interesting properties of myocyte cultures are the ability of myocytes to grow independently of each other and beat at individual rates, or the formation of a synchronously beating network of interconnected heart cells achieved by cell contact and possibly multiplication (7,8).The primary myocyte culture has been used as a model for the automaticity and coordinated rhythmic contractile activity of the intact heart with respect to the action of various chronotropic agents such as drugs and hormones (4, 9, 10). In this report, we show that the rhythmic myocyte contraction in culture is reversibly inhibited by 0.2-2.0 mM N6,02'-dibutyryl cyclic AMP (Bt2 cAMP). Colchicine, an inhibitor of microtubule formation, restores the synchronous contraction of Bt2-cAMP-treated myocytes, although the inactive isomer lumicolchicine does not. In addition, this report presents ultrastructural observations indicating that in Bt2cAMP-treated myocyte cultures the intracellular distribution of microtubules is altered such that these organelles appear to accumulate in parallel arrays, while in control cultures the microtubules appear randomly oriented. MATERIALS AND METHODSPreparation of Myocyte Cultures. Minced ventricular muscles dissected from 19-day-old embryos of Sprague-Dawley rats were disaggregated by repeated incubation with 0.25% pancreat...

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Myocardial and Other Muscle Cell Cultures

Cited by 15 publications

References 132 publications

Cultured Heart Cell Reaggregate Model for Studying Cardiac Toxicology

Cultured Heart Cell Reaggregate Model for Studying Cardiac Toxicology

Cultured heart cell reaggregate model for studying cardiac toxicology.

Relationship between dibutyryl cyclic AMP and microtubule organization in contracting heart muscle cells.

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