Myocardial Infarction: The Protective Role of MiRNAs in Myocardium Pathology

Wang, Wei; Zheng, Hao

doi:10.3389/fcvm.2021.631817

Cited by 17 publications

(17 citation statements)

References 128 publications

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“…Growing evidence has shown that miRNAs are extensively involved in the pathogenic mechanisms that lead to HF in adult patients, such as hypertrophy, apoptosis and fibrosis [20,21].…”

Section: Discussionmentioning

confidence: 99%

Epigenetic Regulation of Cardiac Troponin Genes in Pediatric Patients with Heart Failure Supported by Ventricular Assist Device

et al. 2021

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Ventricular Assist Device (VAD) therapy is considered as a part of standard care for end-stage Heart Failure (HF) children unresponsive to medical management, but the potential role of miRNAs in response to VAD therapy on molecular pathways underlying LV remodeling and cardiac function in HF is unknown. The aims of this study were to evaluate the effects of VAD on miRNA expression profile in cardiac tissue obtained from HF children, to determine the putative miRNA targets by an in-silico analysis as well as to verify the changes of predicated miRNA target in the same cardiac samples. The regulatory role of selected miRNAs on predicted targets was evaluated by a dedicated in vitro study. miRNA profile was determined in cardiac samples obtained from 13 HF children [median: 29 months; 19 LVEF%; 9 Kg] by NGS before VAD implant (pre-VAD) and at the moment of heart transplant (Post-VAD). Only hsa-miR-199b-5p, hsa-miR-19a-3p, hsa-miR-1246 were differentially expressed at post-VAD when compared to pre-VAD, and validated by real-time PCR. Putative targets of the selected miRNAs were involved in regulation of sarcomere genes, such as cardiac troponin (cTns) complex. The expression levels of fetal ad adult isoforms of cTns resulted significantly higher after VAD in cardiac tissue of HF pediatric patients when compared with HF adults. An in vitro study confirmed a down-regulatory effect of hsa-miR-19a-3p on cTnC expression. The effect of VAD on sarcomere organization through cTn isoform expression may be epigenetically regulated, suggesting for miRNAs a potential role as therapeutic targets to improve heart function in HF pediatric patients.

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“…Growing evidence has shown that miRNAs are extensively involved in the pathogenic mechanisms that lead to HF in adult patients, such as hypertrophy, apoptosis and fibrosis [20,21].…”

Section: Discussionmentioning

confidence: 99%

Epigenetic Regulation of Cardiac Troponin Genes in Pediatric Patients with Heart Failure Supported by Ventricular Assist Device

et al. 2021

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“…Increasing evidence has shown that miRNAs are related to cardiac function, disease, and cardioprotection (37)(38)(39)(40). Regarding cardioprotection, the expression of several miRNAs, such as miR-1, miR-21, and miRNA-125b, have been identified to be significantly changed due to ischemic pre-or postconditioning (41)(42)(43)(44).…”

Section: Discussionmentioning

confidence: 99%

Plasma Exosomes at the Late Phase of Remote Ischemic Pre-conditioning Attenuate Myocardial Ischemia-Reperfusion Injury Through Transferring miR-126a-3p

Zhao

Zhang

et al. 2021

Front. Cardiovasc. Med.

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Background: Remote ischemic pre-conditioning (RIPC) alleviated the myocardial ischemia-reperfusion injury, yet the underlying mechanisms remain to be fully elucidated, especially at the late phase. Searching a key component as a transfer carrier may provide a novel insight into RIPC-mediated cardioprotection in the condition of myocardial ischemia-reperfusion.Objective: To investigate the cardioprotective effect of plasma exosomes at the late phase of RIPC and its potential signaling pathways involved.Methods and Results: Exosomes were isolated from the plasma of rats 48 h after the RIPC or control protocol. Although the total plasma exosomes level had no significant change at the late phase of RIPC (RIPC-exosome) compared with the control exosomes (Control-exosome), the RIPC-exosome afforded remarkable protection against myocardial ischemia-reperfusion (MI/R) injury in rats and hypoxia-reoxygenation (H/R) injury in cells. The miRNA array revealed significant enrichment of miR-126a-3p in RIPC-exosome. Importantly, both miR-126a-3p inhibitor and antagonist significantly blunted the cardioprotection of RIPC-exosome in H/R cells and MI/R rats, respectively, while miR-126a-3p mimic and agomir showed significant cardioprotection against H/R injury in cells and MI/R injury in rats. Mechanistically, RIPC-exosome, especially exosomal miR-126a-3p, activated the reperfusion injury salvage kinase (RISK) pathway by enhancing the phosphorylation of Akt and Erk1/2, and simultaneously inhibited Caspase-3 mediated apoptotic signaling.Conclusions: Our findings reveal a novel myocardial protective mechanism that plasma exosomes at the late phase of RIPC attenuate myocardial ischemia-reperfusion injury via exosomal miR-126a-3p.

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“… 128 By binding to the 3’ region of targeted mRNA, miRNAs induce the degradation or silence of that and inhibit the expression of targeted genes. 129 In MI, miRNAs play an essential role in anti-apoptosis or regeneration for cardiomyocytes and inhibition of inflammation for immune cells. Despite significant advances in the use of miRNAs for the regulation of disease pathogenesis, several vital challenges remain to be addressed, ranging from ineffective delivery systems to the continuous host immune response or the potential thrombosis 130 caused by the off-target effects.…”

Section: Nanomaterials For Micro-rna and Stem Cell Deliverymentioning

confidence: 99%

New diagnostic and therapeutic strategies for myocardial infarction via nanomaterials

Shi

Huang

et al. 2022

eBioMedicine

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Myocardial Infarction: The Protective Role of MiRNAs in Myocardium Pathology

Cited by 17 publications

References 128 publications

Epigenetic Regulation of Cardiac Troponin Genes in Pediatric Patients with Heart Failure Supported by Ventricular Assist Device

Epigenetic Regulation of Cardiac Troponin Genes in Pediatric Patients with Heart Failure Supported by Ventricular Assist Device

Plasma Exosomes at the Late Phase of Remote Ischemic Pre-conditioning Attenuate Myocardial Ischemia-Reperfusion Injury Through Transferring miR-126a-3p

New diagnostic and therapeutic strategies for myocardial infarction via nanomaterials

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