Myocardial ultrastructure in systole and diastole using ballistic cryofixation

Monroe, R. Grier; Gamble, Walter J.; Farge, C; Gamboa, R.; Morgan, Clarence L.; Rosenthal, Alan S.; Bullivant, S.

doi:10.1016/s0022-5320(68)90047-6

Cited by 21 publications

(7 citation statements)

References 9 publications

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“…Therefore it is necessary for X-ray microanalytical purposes that the cryofixation process as a whole, starting with the killing of the animals, has to be as fast as possible. Cryoballistic methods (Monroe et al, 1968;Chang et al, 1980) have the potential to minimize both ischaemic and traumatic injury, Until now, however, this has not been demonstrated by X-ray microanalytical measurements.…”

Section: Introductionmentioning

confidence: 99%

Fast cryofixation technique for X‐ray microanalysis

Zglinicki

Rimmler

Purz

1986

Journal of Microscopy

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The proposed cryofixation technique uses a tubule-shaped needle chilled in liquid propane for simultaneous excision and freezing of a tissue specimen. Due to this simultaneity, ionic shifts created by traumatic influences are avoided even in the outermost cells of the specimen.Moreover, it is shown here that stopping the blood flow for more than about 10 s results in notable ionic shifts between cells and extracellular space in rat heart and liver. Such preparative ischaemic injury is minimized by the Fast Cryofixation Technique because it can be easily performed on organs within the circulatory system, whilst the heart of the animal is still beating.Intracellular concentrations of the monovalent ions in rat heart and liver, obtained by this method, tally well with recent results from different independent techniques reported in the literature.As demonstrated by cross-sectioning and freeze-fracturing, the structural preservation of the freezing technique is sufficient for X-ray microanalytical work.

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Section: Introductionmentioning

confidence: 99%

Fast cryofixation technique for X‐ray microanalysis

Zglinicki

Rimmler

Purz

1986

Journal of Microscopy

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“…Our observations on the freeze-fixation of muscle may be compared with previous studies (Monroe et al, 1968;Sjostrom et al, 1973) which have used rapid freezing in order to arrest living muscle cells in either contraction or relaxation.…”

Section: Discussionmentioning

confidence: 57%

Immersion fixation of rapidly frozen, untreated tissues and suspensions of cells including spermatozoa

Cummins

Loper

1975

Journal of Microscopy

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SUMMARY Small pieces of tissue, and cell suspensions in plastic artificial insemination (AI) ‘straws’, were frozen rapidly in Freon‐12 at — 155°C, without pre‐treatment. Peripheral fragments were thawed directly in 2% glutaraldehyde at 0°C, and processed for transmission electron microscopy (TEM) studies. Preservation of ultrastructure was satisfactory, and freezing artifacts were minimal.

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“…Other workers have also used cryofixation techniques to study live contracting muscles but have not described detail at the level of the crossbridges (Monroe et al, 1968; van Harreveld et al, 1974). Nassar et al (1986) performed an outstanding electron microscopic study of the sarcoplasmic reticulum of quick-frozen single fibres of contracting live muscle but without tension measurements.…”

Section: Discussionmentioning

confidence: 99%

Untitled

1988

Journal of Microscopy

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Myocardial ultrastructure in systole and diastole using ballistic cryofixation

Cited by 21 publications

References 9 publications

Fast cryofixation technique for X‐ray microanalysis

Fast cryofixation technique for X‐ray microanalysis

Immersion fixation of rapidly frozen, untreated tissues and suspensions of cells including spermatozoa

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