Myofibroblasts and matrix components in healing palatal wounds in the rat

Cornelissen, A.M.H.; Stoop, Reinout; Hoff, Johannes W. Von den; Maltha, Jaap C.; Kuijpers-Jagtman, Anne Marie

doi:10.1034/j.1600-0714.2000.290101.x

Cited by 31 publications

(21 citation statements)

References 20 publications

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“…In agreement with our results Cornelissen et al, found that normally no alpha SMA was found until 4 days post wounding and the number of positive cells increase from 4 to 8 days post wounding, at 8 or 9 days the majority of the granulation tissue fibroblasts were stained for α SMA and at about 12 days the proportion of α -SMA positive cells are steadily decreased till 22 post wounding where they found no single positive myofibroblast in the newly formed connective tissue (33) .…”

Section: Discussion:-supporting

confidence: 81%

Biological Impact of Melatonin on the Healing of Albino Rats’ Tongue Ulcer.

Amin.¹,

Adel²

2017

IJAR

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Section: Discussion:-supporting

confidence: 81%

Biological Impact of Melatonin on the Healing of Albino Rats’ Tongue Ulcer.

Amin.¹,

Adel²

2017

IJAR

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“…In palatal scar tissue in rats, collagen bundles mainly have a parallel and transversal orientation. 33,34 Because the fibrils in our scaffolds are oriented randomly, this may finally result in a more random pattern of newly formed collagen.…”

Section: Discussionmentioning

confidence: 98%

“…48,49 In palatal wounds of rats, myofibroblasts are mainly present between 4 and 22 days, with a peak intensity after 1 week. 33 On purely morphologic grounds, Dabelsteen and Kremenak 50 described the presence of myofibroblasts in open palatal wounds in dogs between 1 and 2 weeks. Also, in skin wounds, myofibroblasts are abundantly present up to 2 weeks after wounding.…”

Section: Discussionmentioning

confidence: 99%

Collagen Scaffolds Implanted in the Palatal Mucosa

Jansen

Kuijpers-Jagtman²,

Kuppevelt³

et al. 2008

Journal of Craniofacial Surgery

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Scar formation after repair of the cleft palate leads to growth impairment of the upper jaw and midface. The implantation of a suitable scaffold during surgery may reduce this adverse effect. However, little is known about tissue reactions to scaffolds implanted in the oral cavity. Our goal was to analyze the tissue reactions to cross-linked type I collagen scaffolds after submucoperiosteal implantation in the palate of rats. Collagen type I scaffolds were implanted in the palate of 25 male Wistar rats. Groups of 5 rats were killed consecutively after 1, 2, 4, 8, and 16 weeks and were processed for histologic and immunohistochemical analyses. After 1 and 2 weeks, 3 rats from the sham group were also killed. On hematoxylin and eosin-stained sections, the cell density and the number of giant cells were determined. Blood vessels, inflammation, and the presence of myofibroblasts were detected by immunohistochemistry. An influx of inflammatory cells started after 1 week but had completely subsided after 8 weeks. Myofibroblasts were observed within the scaffolds only in the first 2 weeks. Angiogenesis already started after 1 week and showed a peak after 4 weeks, slowly declining afterward. The scaffolds were gradually integrated within the host tissue and only elicited a mild and transient inflammatory response. The scaffolds were biocompatible and seemed to be promising for future applications in cleft palate surgery.

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“…The intervals used to evaluate angiogenesis and the formation of myofibroblasts were 7 and 21 days after the surgical procedure. Myofibroblasts are found in the scar tissue between the fourth and sixth day after injury, with greater proliferation between 8 and 30 day 8 of the healing process 9 .…”

Section: Discussionmentioning

confidence: 98%

Influência da nicotina na proliferação de miofibroblastos e de vasos sanguíneos no tecido cicatricial da parede abdominal de ratos lactentes: estudo imunoistoquímico

Campos

Alves²,

Ioshii

et al. 2010

ABCD, arq. bras. cir. dig.

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-Background -Smoking during pregnancy is detrimental to the intrauterine growth of the child. As in pregnancy, its effects on lactation and breast-fed child is the sum of adverse infant development. Aim -To analyze the effects of nicotine on the proliferation of myofibroblasts and of blood vessels, on the abdominal scar of suckling rats born of mothers that received nicotine, during pregnancy and lactation. Methods -Pregnant Wistar rats were randomly divided into two groups, the nicotine group (NG), in which nicotine was administered subcutaneous at a dose of 2 mg/kg/day, during pregnancy and lactation, and the control group (CG), that received subcutaneous isovolumetric saline solution at 0,9%, in the same period of time. Sixty baby rats, divided into two groups, were weaned at 21 days and a transverse laparotomy was performed. Each group was divided into two subgroups, each one containing 15 baby rats, in accordance with the date when the surgical scar was analyzed: on the 7th post-operative day, control subgroup C7 and nicotine subgroup N7, or on the 21st post-operative day, control subgroup C21 and nicotine subgroup N21. The surgical scar in the healing area was evaluated by immunohistochemistry study for identification of myofibroblasts and of blood vessels. The statistical analysis was based on the Anova model, at a significance level of 5%. Results -The subgroup N7 presented lower number of myofibroblasts (9,93 ± 3,06 vs. 21,87 ± 9,07, p=0,007) and lower number of blood vessels (8,33 ± 4,43 vs. 13,4 ± 5,33, p=0,031) when compared to C7. The subgroup N21 presented no significant difference on the number of myofibroblasts (7,47 ± 3,96 vs. 12,00 ± 7,21, p=0,121) and on the number of blood vessels (9,47 ± 2,42 vs. 12,93 ± 4,35, p=0,090) when compared to C21. Conclusion: Adverse effect of nicotine, administrated in pregnancy and lactation period, was observed on the proliferation of myofibroblasts and of blood vessels on the abdominal wall wound healing of suckling rats.RESUMO -Racional -O tabagismo na gestação acarreta prejuízos para o crescimento intrauterino da criança. Assim como na gestação, as suas consequências sobre a lactação e a criança amamentada constituem somatório de efeitos prejudiciais ao desenvolvimento do lactente. Objetivo -Avaliar a influência da nicotina na proliferação de miofibroblastos e de vasos sanguíneos no tecido cicatricial da parede abdominal de ratos lactentes nascidos e amamentados por ratas que receberam nicotina durante a gestação e lactação. Método -Ratas Wistar prenhas foram divididas aleatoriamente em dois grupos; um grupo recebeu nicotina (GN) na dose diária de 2 mg/kg de peso durante a gestação e lactação; o grupo controle (GC) recebeu solução fisiológica a 0,9%, no mesmo período. Foram utilizados 60 ratos lactentes Wistar, divididos igualmente em dois grupos. Após o término do período de lactação (21 dias), todos foram submetidos à laparotomia transversa e ao fechamento da camada músculo-aponeurótica. Cada grupo foi dividido em dois subgrupos, com 15 animais cad...

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Myofibroblasts and matrix components in healing palatal wounds in the rat

Cited by 31 publications

References 20 publications

Biological Impact of Melatonin on the Healing of Albino Rats’ Tongue Ulcer.

Biological Impact of Melatonin on the Healing of Albino Rats’ Tongue Ulcer.

Collagen Scaffolds Implanted in the Palatal Mucosa

Influência da nicotina na proliferação de miofibroblastos e de vasos sanguíneos no tecido cicatricial da parede abdominal de ratos lactentes: estudo imunoistoquímico

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