Myofibroblasts work best under stress

Wipff, Pierre-Jean; Hinz, Boris

doi:10.1016/j.jbmt.2008.04.031

Cited by 66 publications

(49 citation statements)

References 52 publications

(74 reference statements)

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“…suggest tissue remodelling involving a structural reorganisation favouring greater flexibility consistent with Martin's (2009) study (De Lany et al, 2002;Schleip, 2003;Fourie, 2008Fourie, , 2009. Pioneering research by Wipff and Hinz (2009) on factors determining myofibroblast 'contractile phenotype' retention on maturation, offers appealing supporting evidence, as mechanical stress e such as FRT therapy e is confirmed as one of two principal factors. Even more promising for future SI-based FRT validation in this context is that deregulation of 'normal' fibroblast reparative activities 'results in tissue contracture and development of fibrosis, which makes the fibroblast an important target for anti-fibrotic therapies.'…”

Section: Discussionmentioning

confidence: 99%

Structural integration-based fascial release efficacy in systemic lupus erythematosus (SLE): Two case studies

Ball¹

2011

Journal of Bodywork and Movement Therapies

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Section: Discussionmentioning

confidence: 99%

Structural integration-based fascial release efficacy in systemic lupus erythematosus (SLE): Two case studies

Ball¹

2011

Journal of Bodywork and Movement Therapies

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“…101 Mechanical forces play an important role in normal fibroblast function and, as it turns out, myofibroblast behavior. 101,108 Tensile and compressive forces in diverse tissues and organs, such as the airway wall, heart, and periodontal tissue, promote fibroblast-to-myofibroblast differentiation and myofibroblast function. 15,19,65,104,108 Since the presence of myofibroblasts is a hallmark of many types of cancer, the solid stress generated by tumor growth could be a contributing factor to the induction or potentiation of the tumorassociated fibroblast phenotype.…”

Section: Solid Stress In the Tumor Microenvironmentmentioning

confidence: 99%

“…101,108 Tensile and compressive forces in diverse tissues and organs, such as the airway wall, heart, and periodontal tissue, promote fibroblast-to-myofibroblast differentiation and myofibroblast function. 15,19,65,104,108 Since the presence of myofibroblasts is a hallmark of many types of cancer, the solid stress generated by tumor growth could be a contributing factor to the induction or potentiation of the tumorassociated fibroblast phenotype. Mechanical forces induced indirectly by tumor growth through increased myofibroblast contraction, or even the growth-induced stress itself, may also facilitate neovascularization in the tumor microenvironment by pulling on existing capillaries to form new branches, independent of angiogenesis.…”

Section: Solid Stress In the Tumor Microenvironmentmentioning

confidence: 99%

Biomechanical Forces Shape the Tumor Microenvironment

2011

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The importance of the tumor microenvironment in cancer progression is indisputable, yet a key component of the microenvironment--biomechanical forces--remains poorly understood. Tumor growth and progression is paralleled by a host of physical changes in the tumor microenvironment, such as growth-induced solid stresses, increased matrix stiffness, high fluid pressure, and increased interstitial flow. These changes to the biomechanical microenvironment promote tumorigenesis and tumor cell invasion and induce stromal cells--such as fibroblasts, immune cells, and endothelial cells--to change behavior and support cancer progression. This review highlights what we currently know about the biomechanical forces generated in the tumor microenvironment, how they arise, and how these forces can dramatically influence cell behavior, drawing not only upon studies directly related to cancer and tumor cells, but also work in other fields that have shown the effects of these types of mechanical forces vis-à-vis cell behaviors relevant to the tumor microenvironment. By understanding how all of these biomechanical forces can affect tumor cells, stromal cells, and tumor-stromal crosstalk, as well as alter how tumor and stromal cells perceive other extracellular signals in the tumor microenvironment, we can develop new approaches for diagnosis, prognosis, and ultimately treatment of cancer.

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“…It was predicted that increasing the FBS concentration would increase the generated force, as this was shown when increasing the FBS concentration from 0-10 to 20% (Yee et al 2001;Wakatsuki et al 2000). FBS contains many growth factors, from which transforming growth factor-β (TGF-β) is one that is known to induce cell traction forces (Wipff and Hinz 2009;Hinz 2006;Brown et al 2002). Although TGF-β probably is not the only growth factor in FBS that induces increased traction forces, for this particular growth factor it has been shown that there is an optimum working concentration between 7.5 and 15 ng/ml.…”

Section: Passive and Active Components In Force Generationmentioning

confidence: 99%

Passive and active contributions to generated force and retraction in heart valve tissue engineering

Vlimmeren

Driessen-Mol

Oomens

et al. 2012

Biomech Model Mechanobiol

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In tissue engineered heart valves, cell-mediated stress development during culture results in leaflet retraction at time of implantation. This tissue retraction is partly active due to traction forces exerted by the cells and partly passive due to release of residual stress in the extracellular matrix and the cells. Within this study, we unraveled the passive and active contributions of cells and matrix to generated force and retraction in engineered heart valve tissues. Tissue engineered rectangular strips, fabricated from PGA/P4HB scaffolds and seeded with human myofibroblasts, were cultured for 4 weeks, after which the cellular contribution was changed at different levels. Elimination of the active cellular traction forces was achieved with Cytochalasin D and inhibition of the Rho-associated kinase pathway. Both active and passive cellular contributions were eliminated by lysation and/or decellularization of the tissue. Maximum cell activity was reached by increasing the fetal bovine serum concentration to 50%. The generated force decreased ∼20% after elimination of the active cellular component, ∼25% when the passive cellular component was removed as well and remained unaffected by increased serum concentrations. Passive retraction accounted for ∼60% of total retraction, of which ∼15% was residual stress in the matrix and ∼45% was passive cell retraction. Cell traction forces accounted for the remainder ∼40% of the retraction. Full activation of the cells increased retraction by ∼45%. These results illustrate the importance of the cells in the process of tissue retraction, not only actively retracting the tissue, but also in a passive manner to a large extent.

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Myofibroblasts work best under stress

Cited by 66 publications

References 52 publications

Structural integration-based fascial release efficacy in systemic lupus erythematosus (SLE): Two case studies

Structural integration-based fascial release efficacy in systemic lupus erythematosus (SLE): Two case studies

Biomechanical Forces Shape the Tumor Microenvironment

Passive and active contributions to generated force and retraction in heart valve tissue engineering

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