Myogenesis and muscle regeneration

Yusuf, Faisal; Brand‐Saberi, Beate

doi:10.1007/s00418-012-0972-x

Cited by 52 publications

(40 citation statements)

References 142 publications

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“…Skeletal muscle accounts for 40% of total body mass and demonstrates an innate self-repair capability in response to minor tissue damage or injury [1, 2]. However, regenerating muscle tissues elements capable of spanning segmental muscle gaps or defects following severe injury remains a clinical challenge [3].…”

Section: Introductionmentioning

confidence: 99%

Controlled Heat Stress Promotes Myofibrillogenesis during Myogenesis

Guo¹,

Miller²,

An³

et al. 2016

PLoS ONE

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Hyperthermia therapy has recently emerged as a clinical modality used to finely tune heat stress inside the human body for various biomedical applications. Nevertheless, little is known regarding the optimal timing or temperature of heat stress that is needed to achieve favorable results following hyperthermia therapy for muscle regeneration purposes. The regeneration of skeletal muscle after injury is a highly complex and coordinated process that involves a multitude of cellular mechanisms. The main objective of this study was to characterize the effects of hyperthermal therapy on the overall behavior of myoblasts during myogenic differentiation. Various cellular processes, including myogenesis, myofibrillogenesis, hypertrophy/atrophy, and mitochondrial biogenesis, were studied using systematic cellular, morphological, and pathway-focused high-throughput gene expression profiling analyses. We found that C2C12 myoblasts exhibited distinctive time and temperature-dependence in biosynthesis and regulatory events during myogenic differentiation. Specifically, we for the first time observed that moderate hyperthermia at 39°C favored the growth of sarcomere in myofibrils at the late stage of myogenesis, showing universal up-regulation of characteristic myofibril proteins. Characteristic myofibrillogenesis genes, including heavy polypeptide 1 myosin, heavy polypeptide 2 myosin, alpha 1 actin, nebulin and titin, were all significantly upregulated (p<0.01) after C2C12 cells differentiated at 39°C over 5 days compared with the control cells cultured at 37°C. Furthermore, moderate hyperthermia enhanced myogenic differentiation, with nucleus densities per myotube showing 2.2-fold, 1.9-fold and 1.6-fold increases when C2C12 cells underwent myogenic differentiation at 39°C over 24 hours, 48 hours and 72 hours, respectively, as compared to the myotubes that were not exposed to heat stress. Yet, atrophy genes were sensitive even to moderate hyperthermia, indicating that strictly controlled heat stress is required to minimize the development of atrophy in myotubes. In addition, mitochondrial biogenesis was enhanced following thermal induction of myoblasts, suggesting a subsequent shift toward anabolic demand requirements for energy production. This study offers a new perspective to understand and utilize the time and temperature-sensitive effects of hyperthermal therapy on muscle regeneration.

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Section: Introductionmentioning

confidence: 99%

Controlled Heat Stress Promotes Myofibrillogenesis during Myogenesis

Guo¹,

Miller²,

An³

et al. 2016

PLoS ONE

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“…Although the design of the trial allowed realistic estimation of the long‐term impact of a nutrition intervention provided through the government program, it is possible that the effective dose of supplement was too small due to possible sharing of the supplement with other family members, since consumption of the supplement was not supervised. In addition, the timing of the supplementation may not have been optimal for the programming of muscle mass because the women tended to start collecting the supplements from the ICDS center in the second half of pregnancy, whereas muscle development starts early during fetal development …”

Section: Introductionmentioning

confidence: 99%

Nutritional influences over the life course on lean body mass of individuals in developing countries

2014

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The double burden of childhood undernutrition and adult-onset adiposity in transitioning societies poses a significant public health challenge. The development of suboptimal lean body mass (LBM) could partly explain the link between these two forms of malnutrition. This review examines the evidence on both the role of nutrition in “developmental programming” of LBM and the nutritional influences that affect LBM throughout the life course. Studies from developing countries assessing the relationship of early nutrition with later LBM provide important insights. Overall, the evidence is consistent in suggesting a positive association of early nutritional status (indicated by birth weight and growth during first 2 years) with LBM in later life. Evidence on the impact of maternal nutritional supplementation during pregnancy on later LBM is inconsistent. In addition, the role of nutrients (protein, zinc, calcium, vitamin D) that can affect LBM throughout the life course is described. Promoting optimal intakes of these important nutrients throughout the life course is important for reducing childhood undernutrition as well as for improving the LBM of adults.

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“…The initiation of myotome formation is attributed to the dorsomedial lip (DML), which is known to lay out the scaffold for the early epaxial myotome (Gros et al 2004). The myotome is later populated by myoblasts, which enter the myotome in successive waves from all DM borders (Kahane et al 2002; Hollway and Currie 2005; Yusuf and Brand-Saberi 2012). …”

Section: Discussionmentioning

confidence: 99%

ATOH8, a regulator of skeletal myogenesis in the hypaxial myotome of the trunk

Balakrishnan-Renuka

Morosan-Puopolo

Yusuf

et al. 2013

Histochem Cell Biol

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The embryonic muscles of the axial skeleton and limbs take their origin from the dermomyotomes of the somites. During embryonic myogenesis, muscle precursors delaminate from the dermomyotome giving rise to the hypaxial and epaxial myotome. Mutant studies for myogenic regulatory factors have shown that the development of the hypaxial myotome differs from the formation of the epaxial myotome and that the development of the hypaxial myotome depends on the latter within the trunk region. The transcriptional networks that regulate the transition of proliferative dermomyotomal cells into the predominantly post-mitotic hypaxial myotome, as well as the eventual patterning of the myotome, are not fully understood. Similar transitions occurring during the development of the neural system have been shown to be controlled by the Atonal family of helix-loop-helix transcription factors. Here, we demonstrate that ATOH8, a member of the Atonal family, is expressed in a subset of embryonic muscle cells in the dermomyotome and myotome. Using the RNAi approach, we show that loss of ATOH8 in the lateral somites at the trunk level results in a blockage of differentiation and thus causes cells to be maintained in a predetermined state. Furthermore, we show that ATOH8 is also expressed in cultured C2C12 mouse myoblasts and becomes dramatically downregulated during their differentiation. We propose that ATOH8 plays a role during the transition of myoblasts from the proliferative phase to the differentiation phase and in the regulation of myogenesis in the hypaxial myotome of the trunk. Electronic supplementary materialThe online version of this article (doi:10.1007/s00418-013-1155-0) contains supplementary material, which is available to authorized users.

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Myogenesis and muscle regeneration

Cited by 52 publications

References 142 publications

Controlled Heat Stress Promotes Myofibrillogenesis during Myogenesis

Controlled Heat Stress Promotes Myofibrillogenesis during Myogenesis

Nutritional influences over the life course on lean body mass of individuals in developing countries

ATOH8, a regulator of skeletal myogenesis in the hypaxial myotome of the trunk

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