Myoglianin triggers the pre-metamorphosis stage in hemimetabolan insects

Kamsoi, Orathai; Bellés, Xavier

doi:10.1101/368746

Cited by 1 publication

(7 citation statements)

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“…Firstly, knockdown of Hvmyo delayed larval development by more than 2 days (Figure 3). In agreement with our results, knockdown of myo delays development in B. germanica (Kamsoi & Belles, 2019) and T. castaneum (He et al, 2020). In T. castaneum , for instance, larvae injected with ds myo at the sixth instar stage continue to moult multiple times, and those injected in the seventh instar never moult.…”

Section: Discussionsupporting

confidence: 92%

“…It appears that Myo contributes to the decrease of jhamt mRNA and the JH titre in the final instar of H. vigintioctopunctata larvae. Similarly, Myo inhibits JH production in the CA in hemimetabolous G. bimaculatus (Ishimaru et al, 2016) and B. germanica (Kamsoi & Belles, 2019). In T. castaneum , knockdown of myo prevents the decline of the expression of jhamt3 (He et al, 2020).…”

Section: Discussionmentioning

confidence: 98%

“…Similar phenotypes are observed in the Actβ and daw mutants (Awasaki et al, 2011; Ghosh & O'Connor, 2014; Moss‐Taylor et al, 2019; Zhu et al, 2008). Moreover, the wings and tegmina are somewhat separated in a portion of ds myo ‐treated B. germanica adults (Kamsoi & Belles, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…In hemimetabolous insects, Myo has been documented to regulate the biosynthesis of both 20E and JH (Kamsoi & Belles, 2019). In the cockroach Blattella germanica , myo is highly expressed during the penultimate (fifth) nymphal instar (Ylla et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…In the CA, high Myo levels repress the expression of jhamt . In the PG, a peak of myo expression indirectly stimulates the expression of ecdysone biosynthesis genes, such as neverland , enhancing the production of the metamorphic ecdysone pulse in the final nymphal instar (Kamsoi & Belles, 2019). In the cricket Gryllus bimaculatus , Myo inhibits the expression of jhamt .…”

Section: Introductionmentioning

confidence: 99%

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Requirement of Myoglianin for metamorphosis in the beetleHenosepilachna vigintioctopunctata

Chen

Zhang

et al. 2022

Insect Molecular Biology

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Henosepilachna vigintioctopunctata is a serious defoliating beetle attacking Solanaceae and Cucurbitaceae plants in many Asian countries. In the present paper, we identified a putative myoglianin (myo) gene. Hvmyo was actively transcribed throughout development, from embryo to adult. RNA interference (RNAi)‐aided knockdown of Hvmyo delayed larval development by more than 2 days, reduced larval body size, inhibited the growth of antennae, wings and legs and disturbed gut purge. Knockdown of Hvmyo impaired the larval‐pupal transition. All the Hvmyo RNAi larvae arrested at the larval stage or formed misshapen pupae or adults. The deformed pupae and adults were partially wrapped with exuviae, bearing separated wings. Moreover, the expression levels of five ecdysteroidogenesis genes (Hvspo, Hvphm, Hvdib, Hvsad and Hvshd), a prothocicotropic hormone (PTTH)/Torso pathway gene (Hvtorso), two 20E receptor genes (HvEcR and HvUSP), and two 20E signalling genes (HvE93 and HvFTZ‐F1) were as a result of HvMyo RNAi significantly lowered. Conversely, the expression of a JH biosynthesis gene (Hvjhamt), a JH receptor gene HvMet and a JH signalling gene HvKr‐h1 was greatly enhanced. Although ingestion of 20E and Hal rescued the 20E signal, it could not alleviate larval performance and defective phenotypes. Our results suggest that Myo exerts four distinctive roles in ecdysteroidogenesis, JH production, organ growth and larva‐pupa‐adult transformation in H. vigintioctopunctata.

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Section: Discussionsupporting

confidence: 92%