Myonectin inhibits adipogenesis in 3T3-L1 preadipocytes by regulating p38 MAPK pathway

Abstract

In current times, obesity is a major health problem closely associated with metabolic disease such as diabetes, dyslipidemia, and cardiovascular disease. The direct cause of obesity is known as an abnormal increase in fat cell size and the adipocyte pool. Hyperplasia, the increase in number of adipocytes, results from adipogenesis in which preadipocytes differentiate into mature adipocytes. Adipogenesis is regulated by local and systemic cues that alter transduction pathways and subsequent control of adipogenic transcription factors. Therefore, the regulation of adipogenesis is an important target for preventing obesity. Myonectin, a member of the CTRP family, is a type of myokine released by skeletal muscle cells. Although several studies have shown that myonectin is associated with lipid metabolism, the role of myonectin during adipogenesis is not known. Here, we demonstrate the role of myonectin during adipocyte differentiation of 3T3-L1 cells. We found that myonectin inhibits the adipogenesis of 3T3-L1 preadipocytes with a reduction in the expression of adipogenic transcription factors such as C/EBPα, β and PPARγ. Furthermore, we show that myonectin has an inhibitory effect on adipogenesis through the regulation of the p38 MAPK pathway and CHOP. These findings suggest that myonectin may be a novel therapeutic target for the prevention of obesity.

Methionine sulfoxide suppresses adipogenic differentiation by regulating the mitogen‐activated protein kinase signaling pathway

“…Protein phosphorylation (GO:0006468) and cellular response to insulin stimulus (GO:0032869) were enriched in the MetO-treated group by GO analysis (Figure 4c). The MAPK signaling pathway was enriched in the MetO-treated group compared with control group by KEGG analysis (Figure 4d), which correlated with previous studies on adipogenesis (Park et al, 2021). ERKs, JNKs, and p38 of the MAPK/ERK pathway are detected by western blot analysis.…”

Effects of Intermittent Hypoxia on Cytokine Expression Involved in Insulin Resistance

“…Furthermore, they showed that MN has an inhibitory effect on adipogenesis through the regulation of the p38 mitogen-activated protein kinase (MAPK) pathway and C/EBP homologous protein (CHOP). These results suggest that MN may be a new therapeutic target for obesity prevention [ 90 ] and required for metabolic homeostasis [ 91 ]. In addition, there are various other myokines that are secreted by skeletal muscle and influence pancreatic, hepatic, and adipose tissue and that are thought to affect glucose tolerance through various mechanisms.…”

Germinated Rice Seeds Improved Resveratrol Production to Suppress Adipogenic and Inflammatory Molecules in 3T3-L1 Adipocytes

“…Adipogenesis of 3T3-L1 induced by dexamethasone and IBMX [41] activates adipogenic transcription factors, PPARγ and C/EBPα [42]. During the differentiation of 3T3-L1 fibroblasts to adipocytes, PPARγ and C/EBPα regulate the expression of adipogenic genes such as GLUT-4, FAS, and adiponectin [43,44].…”