Myosin heavy chain composition of tongue muscle in microphthalmic (mi/mi) mice before and after weaning

Yanagisawa, Nobuaki; Abe, Shinichi; Agematsu, Hiroko; Sakiyama, Kazuo; Usami, Akinobu; Tamatsu, Yuichi; Ide, Yoshinobu

doi:10.1016/j.aanat.2006.02.004

Cited by 5 publications

(18 citation statements)

References 25 publications

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“…Such heterogeneity in the site and stage of expression suggested progressive maturation of the muscle fiber attachments. Expression of the myosin heavy chain (Gojo et al 2002;Usami et al 2003;Maejima et al 2005;Shida et al 2005;Okubo et al 2006;Yanagisawa et al 2006;Abe et al 2007;Suzuki et al 2007;Yoshii et al 2008) appeared to have been completed before that of desmin was initiated. Likewise, silver impregnation showed that the endomysium of each muscle fiber was apparently in tight contact with the perichondrium, insertion tendon or intramuscular tendon until the expression of desmin appeared.…”

Section: Discussionmentioning

confidence: 99%

Expression of intermediate filaments at muscle insertions in human fetuses

et al. 2010

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Desmin and vimentin are intermediate filaments that play crucial roles in the maturation, maintenance and recovery of muscle fibers and mesenchymal cells. The expression of these proteins has not been investigated extensively in human fetuses. In the present study, we examined the immunohistochemical expression of intermediate filaments in skeletal muscles of the head, neck and thorax in 12 mid-term human fetuses at 9-18 weeks of gestation. We also used immunohistochemistry to localize the expression of the myosin heavy chain and silver impregnation to identify the fetal endomysium. Expression of desmin and vimentin was already detectable in intercostal muscle at 9 weeks, especially at sites of muscle attachment to the perichondrium. At this stage, myosin heavy chain was expressed throughout the muscle fibers and the endomysium had already developed. Beginning with punctate expression, the positive areas became diffusely distributed in the muscle fibers. At 15-18 weeks, intermediate filament proteins were extensively expressed in all of the muscles examined. Expression at the bone-muscle interface was continuous with expression along the intramuscular tendon fibres. These results suggest that the development of intermediate filaments begins in areas of mechanical stress due to early muscle contraction. Their initially punctate distribution, as observed here, probably corresponds to the earliest stage of fetal enthesis formation.

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Section: Discussionmentioning

confidence: 99%

Expression of intermediate filaments at muscle insertions in human fetuses

et al. 2010

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“…Sartorius et al [ 13 ] reported that skeletal muscles in myosin heavy chain-IId-null mice show increased levels of myosin heavy chain-IIa (MHC-IIa). Yanagisawa et al [ 14 ] have also reported that the presence of MHC-IIa after weaning in microphthalmic (mi/mi) mice with no tooth eruption was attributable to compensation for a lack of proper masticatory function and sucking-like movements, as MHC-IIa is necessary for these movements. In other words, muscle fibers change according to muscle function and structure, and therefore an understanding of the composition of MHC is important when considering the function of the suboccipital muscles.…”

Section: Introductionmentioning

confidence: 99%

Morphological classification and comparison of suboccipital muscle fiber characteristics

et al. 2017

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In an attempt to clarify the function of the suboccipital muscles, we performed morphological observation of the suboccipital muscles for variations in the muscle belly and compared the morphology of their muscle fibers in terms of cross-sectional area by immunostaining with anti-myosin heavy chain antibodies. The cadavers of 25 Japanese individuals were used: 22 for morphological examinations and three for histological examinations. Among samples of the rectus capitis posterior major muscle (RCPma) and rectus capitis posterior minor muscle (RCPmi), 86.4% had a typical muscle appearance with a single belly, and 13.6% had an anomalous morphology. None of the samples of the obliquus capitis superior (OCS) or obliquus capitis inferior (OCI) muscles had an anomalous appearance. Measurement of cross-sectional area revealed that fast-twitch muscle fibers in the RCPma and OCI had a significantly greater cross-sectional area than those of the RCPmi and OCS. The cross-sectional area of intermediate muscle fibers was also significantly greater in the OCS than in the RCPma, RCPmi, and OCI. The cross-sectional area of slow-twitch muscle fibers was significantly greater in the OCS than in the RCPma, RCPmi, and OCI, and the RCPmi showed a significantly greater cross-sectional area for slow-twitch muscle fibers than did the RCPma, and OCI. Our findings indicate that the RCPmi and OCS exert a greater force than the RCPma and OCI, and act as anti-gravity agonist muscles of the head. Prolonged head extension in individuals with anomalous suboccipital muscle groups could result in dysfunction due to undue stress.

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“…As primary antibodies, SC-71 (antiMyHC-2a) and BF-F3 (anti-MyHC-2b) (American Type Tissue Culture), anti-mouse monoclonal antibodies extracted from hybridoma cells, were used 10,[14][15][16]23) . More specifically, after culturing the hybridoma cells in DMEM (10% FBS) medium at 37°C and 5% CO 2 for 72 hours, they were centrifuged, and the supernatants were used as antibodies.…”

Section: Immunofluorescent Stainingmentioning

confidence: 99%

“…Maejima et al and Yanagisawa et al examined the changes of the muscle fiber characteristics of the upper and lower halves of the adult mouse tongue muscle before and after weaning, and confirmed marked changes of isoforms 15,16) . Thus, marked functional changes before and after weaning, i.e., from suckling to chewing, were demonstrated to influence the characteristics of muscle fibers in the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

“…The tongue muscle consists of fast muscle type 5,15,16) ; thus, MyHC-2b and MyHC-2a, which is considered to have the highest and lowest contractile rates among fast muscle type MyHC isoforms, respectively, were selected to investigate the muscle fiber characteristics 4) . HMGB1 was used as a marker to examine whether the carcinoma of tongue actually developed 18) .…”

Section: Introductionmentioning

confidence: 99%

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Characteristics and Effects of Muscle Fibers surrounding Lingual Carcinoma

Sakiyama

Takizawa

Bando

et al. 2013

J. Hard Tissue Biology.

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It has been suggested that dysphagia caused by excision of carcinoma of the tongue may be affected by the hypofunction of the surrounding muscle tissues, as well as by tissue defects. However, there have been no reports concerning how muscle fiber characteristics change in the regions surrounding the lesion after excision of the carcinoma. In this study, we investigated myosin heavy chain (MyHC) isoforms at genetic levels in order to demonstrate the muscle fiber characteristics of the muscle tissues surrounding lingual carcinoma (the experimental group). High mobility group box 1 (HMGB1) was used as a marker to examine whether tongue cancer actually developed. MyHC-2a expression was high in the experimental group. Thus, the marked changes of muscle fiber characteristics, as seen in this study, were considered to be a biological reaction to changing muscle characteristics for regenerating or maintaining normal muscle tissues against the muscle atrophy caused by carcinoma.

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Myosin heavy chain composition of tongue muscle in microphthalmic (mi/mi) mice before and after weaning

Cited by 5 publications

References 25 publications

Expression of intermediate filaments at muscle insertions in human fetuses

Expression of intermediate filaments at muscle insertions in human fetuses

Morphological classification and comparison of suboccipital muscle fiber characteristics

Characteristics and Effects of Muscle Fibers surrounding Lingual Carcinoma

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