Myosin heavy chain-derived peptide ofGadus morhuapromotes proliferation and differentiation in osteoblasts and bone formation and maintains bone homeostasis in ovariectomized mice

Abstract

A novel peptide (MNKKREAEFQ, P-GM-1) screened from Gadus morhua peptides by molecular docking could promote bone formation and maintain bone homeostasis by activating the BMP-2 pathway, and is a potential anti-osteoporosis active ingredient.

Absorption and transport properties of a codfish-derived peptide and its protective effect on bone loss in ovariectomized mice

“…This may be related to the previously reported strong interaction of P-GM-2 with a variety of receptors on the surface of osteocytes. 15 Collectively, these results indicated that P-GM-2 could resist hydrolysis by gastrointestinal proteases and maintain its intact structure in the blood circulation. However, the quantity of these peptides is limited, and most of them will still be hydrolyzed during digestion and absorption, which needs to be studied further.…”

“…14 Meanwhile, screening and characterization of key peptides in GMP revealed that the GETNPADSKPGSIR peptide (P-GM-2) has a strong affinity for integrin. 15 Besides, virtual digestion using the PeptideCutter tool of ExPASy () revealed that P-GM-2 had strong gastrointestinal stability. However, the digestive and absorption properties of P-GM-2 are rarely elucidated, and its anti-osteoporotic activity in vivo remains largely unclear.…”

Novel Peptide Derived from Gadus morhua Stimulates Osteoblastic Differentiation and Mineralization through Wnt/β-Catenin and BMP Signaling Pathways

“…Numerous studies have explored the activation of EGFR as a means to screen for peptides that regulate bone density, theorizing that bioactive peptides stimulating EGFR activation may also promote osteogenesis . In order to more accurately screen peptides with high stability and strong osteogenic activity, the stability of the 10 peptides with the strongest interaction with integrins reported in previous studies was analyzed . In addition, molecular docking technology was employed to further analyze the interaction between these 10 peptides and EGFR.…”

“…20 In order to more accurately screen peptides with high stability and strong osteogenic activity, the stability of the 10 peptides with the strongest interaction with integrins reported in previous studies was analyzed. 19 In addition, molecular docking technology was employed to further analyze the interaction between these 10 peptides and EGFR. The results showed that among these peptides, the peptides of GETNPADSKPGSIR, KSYELPDGQVITIG, and GDSDGDGKIGVDE had higher stability (Table S1).…”

“…Our previous research discovered that GMP demonstrates osteogenic effects by modulating the BMP/WNT signaling pathway. 19 Consequently, we further explored the potential mechanism by which P-GM-2 promotes bone formation by exploring these signaling pathways. During the transduction of the WNT signaling pathway, Wnt-3a binds to its receptor (LRP5/6), resulting in the phosphorylation of GSK-3β and the stable accumulation of β-catenin in cells.…”