Myosin heavy chain isoform transitions in canine skeletal muscles during postnatal growth

Štrbenc, Malan; Smerdu, Vika; Pogačnik, A.; Fazarinc, Gregor

doi:10.1111/j.1469-7580.2006.00599.x

Cited by 13 publications

(14 citation statements)

References 47 publications

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“…3 , 4 ). These persistent populations of hybrid fibers in mouse muscles are consistent with the common occurrence of hybrid fibers in normal muscles from a variety of species [Unguez et al, 2000;Lutz et al, 2001;Stephenson, 2001;Caiozzo et al, 2003;Medler et al, 2004;Strbenc et al, 2006].…”

Section: Fiber Type Transitions With Maturationsupporting

confidence: 54%

“…Next, embryonic MHC is exchanged for neonatal MHC, which is then expressed in conjunction with adult isoforms [Di Maso et al, 2000;Agbulut et al, 2003;Lieber, 2010]. By the time of birth, most muscle fibers express some levels of embryonic and neonatal isoforms in various combinations with one another or with adult isoforms [Di Maso et al, 2000;Unguez et al, 2000;Strbenc et al, 2006;Wank et al, 2006]. Over the subsequent weeks following birth, these developmental isoforms are quickly lost and adult MHC isoform expression increases dramatically.…”

Section: Fiber Type Transitions With Maturationmentioning

confidence: 99%

“…This role is particularly important during early muscle development and subsequent postnatal maturation, when dramatic shifts in fiber type are taking place [Di Maso et al, 2000;Unguez et al, 2000;Strbenc et al, 2006]. During fetal development, skeletal muscle fibers express the embryonic MHC isoform exclusively, or in combination with other isoforms [Di Maso et al, 2000;Agbulut et al, 2003;Lieber, 2010].…”

Section: Fiber Type Transitions With Maturationmentioning

confidence: 99%

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Hybrid Fibers Transform into Distinct Fiber Types in Maturing Mouse Muscles

Brummer

Zhang

Piddoubny

et al. 2013

Cells Tissues Organs

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The role of hybrid fibers as intermediates in fiber type transformations is not completely understood. In some cases hybrids are clearly transitional fibers changing from one type to another, but in others they represent phenotypically stable fibers in normal muscles. In the current study, our goal was to understand the fate of hybrid fibers in fiber type transitions that take place during muscle maturation. Previous studies have reported high proportions of hybrid fibers during postnatal development, but few have followed the fate of these fibers past the time of weaning. We quantified proportions of hybrid fibers in three different mouse skeletal muscles from newly weaned to 6-month-old mice. Hybrid fibers were very prevalent in the brachioradialis (BR) and tibialis anterior (TA) muscles from newly weaned mice, where they constituted 50 and 40% of the fibers, respectively. These hybrids declined steadily to about 15-30% over the next several months. In the soleus muscle the proportion of hybrids did not change, but constituted approximately 20% of fibers. The reduction in IIX/IIB hybrids resulted from different processes in the BR and the TA. In the BR, the reduction was coincident with an increase in type IIX fibers. In the TA, the number of IIX/IIB hybrids was inversely correlated with the proportion of IIB fibers. These patterns reveal that the role of hybrid fibers as intermediates in muscle development is complex. Some hybrid fibers in maturing muscles represent transitional fiber types, while others are phenotypically stable. Moreover, the fate of transitional fibers may be distinct among similar fiber types within different muscles.

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Section: Fiber Type Transitions With Maturationsupporting

confidence: 54%

Section: Fiber Type Transitions With Maturationmentioning

confidence: 99%

Section: Fiber Type Transitions With Maturationmentioning

confidence: 99%

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Hybrid Fibers Transform into Distinct Fiber Types in Maturing Mouse Muscles

Brummer

Zhang

Piddoubny

et al. 2013

Cells Tissues Organs

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“…As expected (Agbulut et al, 2003;Strbenc et al, 2006), postnatal levels of MyHC isoforms EMB and NEO declined to 0% while expression of isoforms IIX and IIB increased to approximately adult values (33.7±3.4% IIX and 63.0±1.2% IIB) by P28 (Fig.·4A). Maximum isometric stress was negatively correlated with levels of EMB and NEO (P<0.001 for each; Fig.·4B,C) but positively correlated with levels of IIX and IIB (P<0.001 for each; Fig.·4F,G).…”

Section: Resultsmentioning

confidence: 60%

“…The principal thick filament constituent of the myofibril is myosin heavy chain (MyHC), and developmental heterogeneity across muscles arises in part from differences in the expression of MyHC isoforms, which include at least two early developmental [embryonic (EMB) and neonatal (NEO)] and four mature (I, IIA, IIX and IIB) isoforms (Bottinelli, 2001;Schiaffino and Reggiani, 1994). Postnatal transitions away from early developmental MyHC isoforms and toward muscle-specific distributions of mature isoforms have been observed in a number of mammalian species (Agbulut et al, 2003;Strbenc et al, 2006;Wank et al, 2006). In the lateral force transmission network, an important constituent is the intermediate filament desmin, which is the first muscle-specific structural protein to be expressed in the embryo (Herrmann et al, 1989;Mayo et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of neonatal skeletal muscle functional improvement in the mouse

Gokhin

Ward

Bremner

et al. 2008

Journal of Experimental Biology

103

102

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SUMMARYPostnatal skeletal muscle growth is classically attributed to fiber hypertrophy and myogenic differentiation, but these processes do not account for the size-independent increase of muscle mechanical performance that occurs during postnatal growth. There is also little knowledge about the precise time-course of contractile function or the underlying factors that affect it. The present study investigated morphological factors (muscle fiber size and myofibrillar packing), biochemical factors (myosin heavy chain isoform and desmin intermediate filament protein expression), and muscle architecture during postnatal development in mice. Physiological testing of the mouse tibialis anterior revealed that maximum isometric stress increased from 27±3·kPa at postnatal day·1 to 169±10·kPa by postnatal day·28, roughly a sixfold increase. Morphological measurements revealed a robust increase in the size-independent packing of myofibrillar matrix material occurring with the functional improvement, with just 48.1±5.5% of the cross-sectional area filled with myofibrils at postnatal day·1 whereas 92.5±0.9% was filled by day·28. Expression of four myosin heavy chain isoforms (embryonic, neonatal, IIX and IIB), as well as desmin, correlated significantly with muscle mechanical function. Stepwise multiple regression showed that, of the variables measured, percentage content of neonatal myosin heavy chain was the best predictor of mechanical function during the postnatal time-course. These data provide the first specific structural basis for increases in muscle tension development during growth. Therefore, models of muscle growth must be modified to include an intrinsic quality enhancement component.

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Enzyme‐ and immunohistochemical aspects of skeletal muscle fibers in brown bear (Ursus arctos)

Smerdu¹,

Cehovin

Štrbenc

et al. 2008

Journal of Morphology

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To further elucidate the pattern of MHC isoform expression in skeletal muscles of large mammals, in this study the skeletal muscles of brown bear, one of the largest mammalian predators with an extraordinary locomotor capacity, were analyzed. Fiber types in longissimus dorsi, triceps brachii caput longum, and rectus femoris muscles were determined according to the myofibrillar ATPase (mATPase) histochemistry and MHC isoform expression, revealed by a set of antibodies specific to MHC isoforms. The oxidative (SDH) and glycolytic enzyme (alpha-GPDH) capacity of fibers was demonstrated as well. By mATPase histochemistry five fiber types, i.e., I, IIC, IIA, IIAX, IIX were distinguished. Analyzing the MHC isoform expression, we assume that MHC-I, -IIa, and -IIx are expressed in the muscles of adolescent bears. MHC-I isoform was expressed in Type-I fibers and coexpressed with presumably -IIa isoform, in Type-IIC fibers. Surprisingly, two antibodies specific to rat MHC-IIa stained those fast fibers, that were histochemically and immunohistochemically classified as Type IIX. This assumption was additionally confirmed by complete absence of fiber staining with antibody specific to rat MHC-IIb and all fast fiber staining with antibody that according to our experience recognizes MHC-IIa and -IIx of rat. Furthermore, quite high-oxidative capacity of all fast fiber types and their weak glycolytic capacity also imply for MHC-IIa and -IIx isoform expression in fast fibers of bear. However, in adult, full-grown animal, only MHC-I and MHC-IIa isoforms were expressed. The expression of only two fast isoforms in bear, like in many other large mammals (humans, cat, dog, goat, cattle, and horse) obviously meets the weight-bearing and locomotor demands of these mammals.

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Myosin heavy chain isoform transitions in canine skeletal muscles during postnatal growth

Cited by 13 publications

References 47 publications

Hybrid Fibers Transform into Distinct Fiber Types in Maturing Mouse Muscles

Hybrid Fibers Transform into Distinct Fiber Types in Maturing Mouse Muscles

Quantitative analysis of neonatal skeletal muscle functional improvement in the mouse

Enzyme‐ and immunohistochemical aspects of skeletal muscle fibers in brown bear (Ursus arctos)

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