Myosin Motor-Like Domain of Class VI Chitin Synthase CsmB of &lt;i&gt;Aspergillus nidulans&lt;/i&gt; Is Not Functionally Equivalent to That of Class V Chitin Synthase CsmA

Tsuizaki, Makusu; Ohta, Akio; Horiuchi, Hiroyuki

doi:10.1271/bbb.120822

Cited by 13 publications

(6 citation statements)

References 24 publications

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“…The phenotypic properties of chsVII mutants support the uniqueness of the class VII of Chs, since a differential phenotype was observed that could not be restored by the other Chs. It has been published recently that the MMD of class VII is not functionally equivalent to the MMD of class V (Tsuizaki et al, 2013) which underlines the different role of the shorter MMD domain and the different functionality of each class.…”

Section: Discussionmentioning

confidence: 98%

The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum

Gandía¹,

Harries²,

Marcos³

2014

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 98%

The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum

Gandía¹,

Harries²,

Marcos³

2014

Fungal Genetics and Biology

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“…The MMDs of CsmA and CsmB bound to actin filaments in vitro [ 20 , 25 ] and the interaction between each MMD and actin was crucial for their proper localization and function [ 25 , 26 ]. However, the MMD of CsmB is not functionally equivalent to that of CsmA [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Transportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin

et al. 2015

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Cell wall formation and maintenance are crucial for hyphal morphogenesis. In many filamentous fungi, chitin is one of the main structural components of the cell wall. Aspergillus nidulans ChsB, a chitin synthase, and CsmA, a chitin synthase with a myosin motor-like domain (MMD) at its N-terminus, both localize predominantly at the hyphal tip regions and at forming septa. ChsB and CsmA play crucial roles in polarized hyphal growth in A. nidulans. In this study, we investigated the mechanism by which CsmA and ChsB accumulate at the hyphal tip in living hyphae. Deletion of kinA, a gene encoding conventional kinesin (kinesin-1), impaired the localization of GFP-CsmA and GFP-ChsB at the hyphal tips. The transport frequency of GFP-CsmA and GFP-ChsB in both anterograde and retrograde direction appeared lower in the kinA-deletion strain compared to wild type, although the velocities of the movements were comparable. Co-localization of GFP-ChsB and GFP-CsmA with mRFP1-KinArigor, a KinA mutant that binds to microtubules but does not move along them, was observed in the posterior of the hyphal tip regions. KinA co-immunoprecipitated with ChsB and CsmA. Co-localization and association of CsmA with KinA did not depend on the MMD. These findings indicate that ChsB and CsmA are transported along microtubules to the subapical region by KinA.

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“…Bacterial and fungal transformations were performed as described previously. 26) Isolation of total RNA from A. nidulans. The conidia of the strain A1149 were inoculated into YGuu medium and cultured for 24 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

A Wiskott–Aldrich syndrome protein is involved in endocytosis in Aspergillus nidulans

Hoshi

Zheng

Ohta

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Endocytosis is vital for hyphal tip growth in filamentous fungi and is involved in the tip localization of various membrane proteins. To investigate the function of a Wiskott-Aldrich syndrome protein (WASP) in endocytosis of filamentous fungi, we identified a WASP ortholog-encoding gene, wspA, in Aspergillus nidulans and characterized it. The wspA product, WspA, localized to the tips of germ tubes during germination and actin rings in the subapical regions of mature hyphae. wspA is essential for the growth and functioned in the polarity establishment and maintenance during germination of conidia. We also investigated its function in endocytosis and revealed that endocytosis of SynA, a synaptobrevin ortholog that is known to be endocytosed at the subapical regions of hyphal tips in A. nidulans, did not occur when wspA expression was repressed. These results suggest that WspA plays roles in endocytosis at hyphal tips and polarity establishment during germination.

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Myosin Motor-Like Domain of Class VI Chitin Synthase CsmB of <i>Aspergillus nidulans</i> Is Not Functionally Equivalent to That of Class V Chitin Synthase CsmA

Cited by 13 publications

References 24 publications

The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum

The myosin motor domain-containing chitin synthase PdChsVII is required for development, cell wall integrity and virulence in the citrus postharvest pathogen Penicillium digitatum

Transportation of Aspergillus nidulans Class III and V Chitin Synthases to the Hyphal Tips Depends on Conventional Kinesin

A Wiskott–Aldrich syndrome protein is involved in endocytosis in Aspergillus nidulans

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