Myosins and DYNLL1/LC8 in the honey bee (Apis mellifera L.) brain

Calábria, Luciana Karen; Peixoto, Pablo Marco Veras; Lima, Andreia Barcelos Passos; Peixoto, Leonardo Gomes; Moraes, Viviane Rodrigues Alves de; Teixeira, Renata Roland; Santos, Catarina; Silva, Letícia Oliveira e; Silva, Maria de Fátima Rodrigues da; Santos, Ana Alice D.; García-Cairasco, Norberto; Martins, Antônio Roberto; Espreáfico, Enilza Maria

doi:10.1016/j.jinsphys.2011.06.005

Cited by 4 publications

(2 citation statements)

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“…This ncRNA is located proximal to LOC410058, a myosin light chain alkali-like gene. Myosins are key proteins in the nervous and visual systems of honey bees [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Genomic Variants Associated with Scout and Recruit Behavioral Castes in Honey Bees Using Whole-Genome Sequencing

et al. 2016

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Among forager honey bees, scouts seek new resources and return to the colony, enlisting recruits to collect these resources. Differentially expressed genes between these behaviors and genetic variability in scouting phenotypes have been reported. Whole-genome sequencing of 44 Apis mellifera scouts and recruits was undertaken to detect variants and further understand the genetic architecture underlying the behavioral differences between scouts and recruits. The median coverage depth in recruits and scouts was 10.01 and 10.7 X, respectively. Representation of bacterial species among the unmapped reads reflected a more diverse microbiome in scouts than recruits. Overall, 1,412,705 polymorphic positions were analyzed for associations with scouting behavior, and 212 significant (p-value < 0.0001) associations with scouting corresponding to 137 positions were detected. Most frequent putative transcription factor binding sites proximal to significant variants included Broad-complex 4, Broad-complex 1, Hunchback, and CF2-II. Three variants associated with scouting were located within coding regions of ncRNAs including one codon change (LOC102653644) and 2 frameshift indels (LOC102654879 and LOC102655256). Significant variants were also identified on the 5’UTR of membrin, and 3’UTRs of laccase 2 and diacylglycerol kinase theta. The 60 significant variants located within introns corresponded to 39 genes and most of these positions were > 1000 bp apart from each other. A number of these variants were mapped to ncRNA LOC100578102, solute carrier family 12 member 6-like gene, and LOC100576965 (meprin and TRAF-C homology domain containing gene). Functional categories represented among the genes corresponding to significant variants included: neuronal function, exoskeleton, immune response, salivary gland development, and enzymatic food processing. These categories offer a glimpse into the molecular support to the behaviors of scouts and recruits. The level of association between genomic variants and scouting behavior observed in this study may be linked to the honey bee’s genomic plasticity and fluidity of transition between castes.

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“…This ncRNA is located proximal to LOC410058, a myosin light chain alkali-like gene. Myosins are key proteins in the nervous and visual systems of honey bees [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of Genomic Variants Associated with Scout and Recruit Behavioral Castes in Honey Bees Using Whole-Genome Sequencing

et al. 2016

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“…The CBAS contains numerous Syp-immunoreactive puncta (Katow et al, 2016), suggesting neurosecretory activity of the organ. In addition to the sea urchin, Syp has been reported in vast phylogenetic groups of invertebrates, such as the flatworm (Schistosoma mansoni; Rabelo et al, 1997), C. elegans (Abraham et al, 2011), insects (Calábria, et al, 2011;Stevens et al, 2012), Aplysia (Jin et al, 2011) and Octopus (Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Ontogeny of a synaptophysin-mediated GABA transmission mechanism from the ciliary band-associated strand to the ciliary band during the development of the sea urchin Hemicentrotus pulcherrimus

Katow

Yoshida

Katow

et al. 2018

Preprint

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Swimming activity of the sea urchin larva depends on ciliary beating primarily in the circumoral ciliary band (CB) and is regulated by several neurotransmitters, such as 5HT, dopamine and γ-aminobutyric acid (GABA). Accordingly, the larval swimming activity is severely inhibited by 3-mercaptopropionic acid [a glutamate decarboxylase (GAD) inhibitor]. Although GABA is detected in the CB, GAD is absent. GAD is expressed in the spatially segregated nearby ciliary band-associated strand (CBAS). Thus, it is assumed that GABA transmission extends from the CBAS to the CB. Here, we examined the synaptic transmission mechanism by focusing on the spatiotemporal expression pattern of synaptophysin (Syp), a synaptic vesicle glycoprotein. The sea urchin has a single copy of the Syp gene, which encodes a 266-amino acid protein with possibly 4 transmembrane domains. We generated an anti-Syp antibody (Ab).Immunoblotting (IB) detected Ab binding to a single band at approximately 38 kDa.Whole-mount immunohistochemistry (WMIHC) detected high intensity Ab binding to the CBAS. Syp was initially detected at the mesenchyme blastula stage (mBL) as a single band by IB. Accordingly, WMIHC detected Syp in the cytoplasm of small patches of several ectodermal cells at the mBL stage. Syp has also been detected in the cytoplasm of blastocoelar cells from the prism stage to the 2-arm pluteus stage (2aPL). By the 4aPL stage, Syp was expressed in the CBAS and was moderately expressed among the blastocoelar cells. Distinctive co-localization of GABA and Syp was not detected until the 2aPL stage. Beginning at the 4aPL stage, GABA was detected in the CB and Syp-positive puncta in the CBAS. In the CB, GABA was co-localized with the GABA-A receptor (GABA A R). Thus, the GABA signal may be transmitted from GAD in the CBAS through a Syp-mediated system to the CB and then, in the CB, to the basal body of the cilia through GABA A R.

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Identification and characterization of a novel calcyclin binding protein (CacyBP) gene from Apis cerana cerana

Sun

et al. 2012

Mol Biol Rep

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Calcyclin binding protein (CacyBP), a homolog of Sgt1, was shown to interact with some S100 proteins, Skp1, tubulin, actin and ERK1/2 kinases. Studies have also shown that CacyBP is a neuronal protein in mammals. Limited information is available regarding the properties and functions of CacyBP in insects. Here, we cloned and characterized a novel CacyBP gene, named AccCacyBP, from honeybee (Apis cerana cerana). Bioinformatic analysis indicated that AccCacyBP was highly conserved and closely related to the CacyBP of other insects. Promoter analysis revealed a number of putative tissue, development and stress-related transcription factor-binding sites. RT-qPCR demonstrated that AccCacyBP was expressed at all of the stages of development, especially in the brains of honeybees. Moreover, immunohistochemistry analysis showed the presence of AccCacyBP in the brain. The transcript levels of AccCacyBP in the brains of honeybees were developmentally induced and upregulated by exposure to oxidative stresses, including UV-light, acetamiprid and HgCl(2). This study demonstrates that the CacyBP gene in honeybees may be a neuronal protein involved in the developmental regulation and the stress-response of the brain of honeybees.

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Myosins and DYNLL1/LC8 in the honey bee (Apis mellifera L.) brain

Cited by 4 publications

References 97 publications

Characterization of Genomic Variants Associated with Scout and Recruit Behavioral Castes in Honey Bees Using Whole-Genome Sequencing

Characterization of Genomic Variants Associated with Scout and Recruit Behavioral Castes in Honey Bees Using Whole-Genome Sequencing

Ontogeny of a synaptophysin-mediated GABA transmission mechanism from the ciliary band-associated strand to the ciliary band during the development of the sea urchin Hemicentrotus pulcherrimus

Identification and characterization of a novel calcyclin binding protein (CacyBP) gene from Apis cerana cerana

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