Myostatin mRNA expression and its association with body weight and carcass traits in Yunnan Wuding chicken

Liu, L X; Dou, Tengfei; Li, Q H; Hua, Rong; Tong, Huiquan; Xu, Zhiqiang; Huang, Ying; Gu, Dahai; Chen, X B; Ge, Chao; Jia, Junjing

doi:10.4238/gmr15048967

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…Among the DEGs involved in growth and development identified in this study, some were previously reported to be closely related to muscle growth and development. As a regulator of skeletal muscle growth, MSTN plays a key role in negatively regulating the growth and development of skeletal muscle and influencing the strength and quality of muscles (Yi et al, 2016;Liu et al, 2016;Rao et al, 2016). MSTN can inhibit the activation, proliferation, and differentiation of myogenic satellite cells (McFarland et al, 2006), and delayed differentiation of myoblasts due to a lack of MSTN results in significant changes in the morphology of myoblasts (Sato et al, 2006), which indicates that MSTN plays an important role in chicken embryogenesis and skeletal muscle development and is one of the important factors regulating muscle development.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken

Yin

Zhang

et al. 2021

Arch. Anim. Breed.

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Abstract. Skeletal muscle growth and development from embryo to adult consists of a series of carefully regulated changes in gene expression. This study aimed to identify candidate genes involved in chicken growth and development and to investigate the potential regulatory mechanisms of early growth in Haiyang yellow chicken. RNA sequencing was used to compare the transcriptomes of chicken muscle tissues at four developmental stages. In total, 6150 differentially expressed genes (DEGs) (|fold change| ≥ 2; false discovery rate (FDR) ≤ 0.05) were detected by pairwise comparison in female chickens. Functional analysis showed that the DEGs were mainly involved in the processes of muscle growth and development and cell differentiation. Many of the DEGs, such as MSTN, MYOD1, MYF6, MYF5, and IGF1, were related to chicken growth and development. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the DEGs were significantly enriched in four pathways related to growth and development: extracellular matrix (ECM)–receptor interaction, focal adhesion, tight junction, and insulin signalling pathways. A total of 42 DEGs assigned to these pathways are potential candidate genes for inducing the differences in growth among the four development stages, such as MYH1A, EGF, MYLK2, MYLK4, and LAMB3. This study identified a range of genes and several pathways that may be involved in regulating early growth.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken

Yin

Zhang

et al. 2021

Arch. Anim. Breed.

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“…Moreover, MSTN was regulated development of muscle fiber (Liu et al 2016). Liu et al (2016) exhibited that mRNA level of MSTN in breast muscle was lower in Wuding chicken than in broilers before 30 day. In contrast, after 30day, themRNA level was higher in breast muscle of Wuding chicken more than in broilers.…”

Section: Gene Expressionmentioning

confidence: 94%

“…Myostatin prevents proliferation of myoblast (Bass et al 1999) by inhibiting their cell cycle progression from the G1 to the S phase (Thomas et al 2000). Liu et al (2016) reported that the MSTN gene may be useful in molecular breeding in chicken owing to its negative regulatory effect on muscle mass.In chicken, Kocamis and Killefer (2002) showed that MSTN gene expression is increased in skeletal muscles during the second half of embryonic development and decline on hatching day. Furthermore, it lower expression in muscle of 30-day-old (Liu et al 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Liu et al (2016) reported that the MSTN gene may be useful in molecular breeding in chicken owing to its negative regulatory effect on muscle mass.In chicken, Kocamis and Killefer (2002) showed that MSTN gene expression is increased in skeletal muscles during the second half of embryonic development and decline on hatching day. Furthermore, it lower expression in muscle of 30-day-old (Liu et al 2016). They may contribute to the onset of skeletal muscle development in newly hatched chicks, which is dependent of the fast initiation of neonatal metabolism (Mott and Ivarie, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Estimated Gene Expression in Meat Quality Trait Under Stress Conditions for Two Broiler Strains

Nasser

Radwan

Mahrous

et al. 2019

Arab Universities Journal of Agricultural Sciences

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The objective of this study was to estimated gene expression in breast muscles of two broiler strains with used to Myoustatin gene in marketing age (5 weeks of age) under heat stress and compared to control group for 1 hour daily for 7 days in Shalakan farm of Faculty of Agriculture, Ain Shams University, broiler chicks (150 chicks in each strains) of 1 day of age were used in this study. So, the measurements were taken (respiratory rate, rectal temperature and estimated gene expression). The results obtained the gene expression of Myostatin gene in muscle breast was observed highest in Ross strain compared with Indian River strain under heat stress, but no different between strains ( Ross and Indian River) in control group. However, respiratory rate of Ross and Indian River under heat at 5 week of age showed that the Indian River strain was high significant at 5 week of age compared to Ross ones. As shown that rectal temperature of Ross and Indian River were a high significant effect of treatment (heat group was higher from control group) and not a significant of strain.The present experiment was conducted to estimated gene expression on meat quality traits of different tropical stress conditions in different broiler strains during 7 days and 5 weeks of age. Introgressions some major genes likes myostatin gene in muscle breast into broiler improve under the heat stress.

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“…The growth of broilers is generally influenced by environment and heredity, and nowadays, under the condition of industrialized production, the most important factor affecting the growth of broilers is heredity. Growth trait is a quantitative trait regulated by multiple genes (Goto et al, 2019), such as myogenic regulatory factors (MRFs) (Zhu et al, 2016), insulin like growth factor 1/insulin like growth factor 1 receptor (IGF-I/IGF-IR) (Roelfsema et al, 2018), myostatin (MSTN) (Liu et al, 2016), etc. Therefore, it is necessary to study the molecular mechanism of growth regulation in poultry.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #2 of "Transcriptome for the breast muscle of Jinghai yellow chicken at early growth stages (v0.1)"

2020

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Background: The meat quality of yellow feathered broilers is better than the quality of its production. Growth traits are important in the broiler industry. The exploration of regulation mechanisms for the skeletal muscle would help to increase the growth performance of chickens. At present, some progress has been made by researchers, but the molecular mechanisms of the skeletal muscle still remain unclear and need to be improved. Methods: In this study, the breast muscles of fast-and slow-growing female Jinghai yellow chickens (F4F, F8F, F4S, F8S) and slow-growing male Jinghai yellow chickens (M4S, M8S) aged four and eight weeks were selected for transcriptome sequencing (RNAseq). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected nine DEGs to verify the accuracy of the sequencing by qPCR. Results: The differential gene expression analysis resulted in 364, 219 and 111 DEGs (adjusted P-value ≤0.05) for the three comparison groups, F8FvsF4F, F8SvsF4S, and M8SvsM4S, respectively. Three common DEGs (ADAMTS20, ARHGAP19, and Novel00254) were found , and they were all highly expressed at four weeks of age. In addition, some other genes related to growth and development, such as ANXA1, COL1A1, MYH15, TGFB3 and ACTC1, were obtained. The most common DEGs (n =58) were found between the two comparison groups F8FvsF4F and F8SvsF4S, and they might play important roles in the growth of female chickens. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway also showed some significant enrichment pathways, for instance, extracellular matrix (ECM)-receptor interaction, focal adhesion, cell cycle, and DNA replication. The two pathways that were significantly enriched in the F8FvsF4F group were all contained in that of F8SvsF4S. The same two pathways were ECM-receptor interaction and focal adhesion, and they had great influence on the growth of chickens. However, many differences existed between male and female chickens in regards to common DEGs and KEGG pathways. The results would help to reveal the regulation mechanism of the growth and

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Myostatin mRNA expression and its association with body weight and carcass traits in Yunnan Wuding chicken

Cited by 18 publications

References 32 publications

Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken

Transcriptomic profile of leg muscle during early growth and development in Haiyang yellow chicken

Estimated Gene Expression in Meat Quality Trait Under Stress Conditions for Two Broiler Strains

Peer Review #2 of "Transcriptome for the breast muscle of Jinghai yellow chicken at early growth stages (v0.1)"

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