Myricetin Apoptotic Effects on T47D Breast Cancer Cells is a P53-Independent Approach

Soleimani, Mitra; Sajedi, Nayereh

doi:10.31557/apjcp.2020.21.12.3697

Cited by 12 publications

(11 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LncRNA-ENST00000421645 was overexpressed in a T lymphocyte line (Jurkat-E6-1), and lncRNA-ENST00000421645 was found to promote the expression of the apoptosis-related protein caspase3 in T lymphocytes and promote T lymphocyte apoptosis. Caspase3 is a major effector caspase and is a common downstream effector molecule of multiple apoptotic pathways that plays a central role in the process of apoptosis; thus, it is called a “death executing protease ( Li et al, 1997 ; Porter and Jänicke, 1999 ; Winstel et al, 2019 ; Soleimani and Sajedi, 2020 ). Our findings suggested that lncRNA-ENST00000421645 induced apoptosis in T lymphocytes through the caspase pathway.…”

Section: Discussionmentioning

confidence: 99%

LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

Wang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Long non-coding RNAs are involved in many infectious diseases. Our previous studies showed that lncRNA-ENST00000421645 expression is increased in T lymphocytes of neurosyphilis patients compared to healthy controls. However, whether lncRNA-ENST00000421645 has biological functions remains unclear. The current study was undertaken to understand the mechanism of lncRNA-ENST00000421645 in T lymphocyte function in neurosyphilis patients. The lncRNA-ENST00000421645 pull-down assay showed that lncRNA-ENST00000421645 acted on the acetylase NAT10. The chromatin immunoprecipitation (ChIP)-PCR results showed that lncRNA-ENST00000421645 promoted the acetylation of histone H3K27 adjacent to the Kank1 promoter, thereby promoting Kank1 protein expression. Kank1 promotes 14-3-3 protein expression, inhibits NF-kB activation, inhibits IFN-γ secretion by T lymphocytes, and promotes T lymphocyte apoptosis. Taken together, our findings suggest a novel mechanism that LncRNA-ENST00000421645 upregulates Kank1 to inhibit IFN-γ expression and promote T cell apoptosis in neurosyphilis.

show abstract

Section: Discussionmentioning

confidence: 99%

LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

Wang

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Myricetin-induced cell apoptosis has been observed in T47D breast cancer cells, associated with increased expression of apoptotic genes including caspase-3, -8, -9, BAX/Bcl-2, p53, BRCA1, and GADD45. In particular, the BRCA1-GADD45 pathway was involved in the apoptotic death of the T47D cells [141]. Myricetin has also been reported to inhibit the migration and invasion of hepatocellular carcinoma MHCC97H cells and weaken filopodia and lamellipodia [142].…”

Section: Myricetinmentioning

confidence: 99%

“…Bax, Caspase-3, Caspase-8, Caspase-9, p53, BRCA1, GADD45, and E-Cad Prostate [132], ovarian [133], skin [134], breast [135,139], liver [136,137,140], colorectal [138], and thyroid [141] cancer ↓ PIM1, CXCR4, MDR-1, Bcl-2, VEGFR2, p38, p-STAT3, COX2, IL-6, TNF-α, NFκB, and N-Cad…”

mentioning

confidence: 99%

The Anti-Cancer Effects of Red-Pigmented Foods: Biomarker Modulation and Mechanisms Underlying Cancer Progression

2022

View full text Add to dashboard Cite

Cancer is one the most malignant diseases that is a leading cause of death worldwide. Vegetables and fruits contain beneficial nutrients such as vitamins, minerals, folates, dietary fibers, and various natural bioactive compounds. These can prevent the pathological processes of many cancers and reduce cancer related mortality. Specifically, the anti-cancer effect of vegetables and fruits is largely attributable to the natural bioactive compounds present within them. A lot of bioactive compounds have very specific colors with pigments and the action of them in the human body varies by their color. Red-pigmented foods, such as apples, oranges, tomatoes, cherries, grapes, berries, and red wine, have been widely reported to elicit beneficial effects and have been investigated for their anti-tumor, anti-inflammatory, and antioxidative properties, as well as anti-cancer effect. Most of the anti-cancer effects of bioactive compounds in red-pigmented foods arise from the suppression of cancer cell invasion and metastasis, as well as the induction of apoptosis and cell cycle arrest. In this review, we assessed publications from the last 10 years and identified 10 bioactive compounds commonly studied in red-pigmented foods: lycopene, anthocyanin, β-carotene, pectin, betaine, rutin, ursolic acid, kaempferol, quercetin, and myricetin. We focused on the mechanisms and targets underlying the anti-cancer effect of the compounds and provided rationale for further investigation of the compounds to develop more potent anti-cancer treatment methods.

show abstract

“…Previous studies have demonstrated the anti-oxidant, antiviral, antibacterial, and anticancer effects of myricetin (10)(11)(12). Myricetin has been found to exhibit anticancer effects against pancreatic (13), liver (14), prostate (15), thyroid (16) and breast cancer (17)(18)(19) in vitro. In particular, myricetin targets the mitochondrial apoptosis pathway to inhibit cellular proliferation and induce apoptosis (20).…”

Section: Introductionmentioning

confidence: 99%

Myricetin induces apoptosis through the MAPK pathway and regulates JNK‑mediated autophagy in SK‑BR‑3 cells

et al. 2022

View full text Add to dashboard Cite

Myricetin, a flavonoid found in fruits and vegetables, is known to have antioxidant and anticancer effects. However, the anticancer effects of myricetin on SK-BR-3 human breast cancer cells have not been elucidated. In the present study, the anticancer effects of myricetin were confirmed in human breast cancer SK-BR-3 cells. As the concentration of myricetin increased, the cell viability decreased. DAPI (4',6-diamidino-2-phenylindole) and Annexin V/PI staining also revealed a significant increase in apoptotic bodies and apoptosis. Western blot analysis was performed to confirm the myricetin-induced expression of apoptosis-related proteins. The levels of cleaved PARP and Bax proteins were increased, and that of Bcl-2 was decreased. The levels of proteins in the mitogen-activated protein kinase (MAPK) pathway were examined to confirm the mechanism of myricetin-induced apoptosis, and it was found that the expression levels of phosphorylated c-Jun N-terminal kinase (p-JNK) and phosphorylated mitogen-activated protein kinases (p-p38) were increased, whereas that of phosphorylated extracellular-regulated kinase (p-ERK) was decreased. It was also demonstrated that myricetin induced autophagy by promoting autophagy-related proteins such as microtubule-associated protein 1A/1B-light chain 3 (LC 3) and beclin 1. In addition, 3-methyladenine (3-MA) was used to evaluate the association between cell viability and autophagy in cells treated with myricetin. The results showed that simultaneous treatment with 3-MA and myricetin promoted the apoptosis of breast cancer cells. Furthermore, treatment with a JNK inhibitor reduced cell viability, promoted Bax expression, and reduced the expression of p-JNK, Bcl-2, and LC 3-II/I. These results suggest that myricetin induces apoptosis via the MAPK pathway and regulates JNK-mediated autophagy in SK-BR-3 cells. In conclusion, myricetin shows potential as a natural anticancer agent in SK-BR-3 cells.

show abstract

Myricetin Apoptotic Effects on T47D Breast Cancer Cells is a P53-Independent Approach

Cited by 12 publications

References 42 publications

LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

LncRNA-ENST00000421645 Upregulates Kank1 to Inhibit IFN-γ Expression and Promote T Cell Apoptosis in Neurosyphilis

The Anti-Cancer Effects of Red-Pigmented Foods: Biomarker Modulation and Mechanisms Underlying Cancer Progression

Myricetin induces apoptosis through the MAPK pathway and regulates JNK‑mediated autophagy in SK‑BR‑3 cells

Contact Info

Product

Resources

About