Myricetin Attenuates LPS-induced Inflammation in RAW 264.7 Macrophages and Mouse Models

Hou, Wei; Hu, Siyi; Su, Zheng; Wang, Qi; Meng, Guangping; Guo, Tingting; Zhang, Jie; Gao, Peng

doi:10.1101/299420

Cited by 13 publications

(13 citation statements)

References 31 publications

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“…Inflammation caused by IFN-γ was reported to be suppressed due to the regulatory matory cytokines . The inhibition of inflammation related genes as observed in the present study were in line with an earlier report, where the exposure to myricetin before LPS induction decreased the inflammatory activity in RAW264.7 macrophages [19]. This antiinflammatory effect may due myricetin capacity to suppress pro-inflammatory mediators' production by inhibiting NF-κB and STAT-1 signals pathways.…”

Section: Discussionsupporting

confidence: 93%

“…The EZ-Cytox cell viability assay kit was used and cytotoxicity of RAW264.7 macrophages was not shown [18]. Houa et al had also reported that RAW264.7 macrophages exposed to LPS demonstrated no cytotoxicity following MTT assay when pretreated with 25 μM of myricetin [19]. The protective effects of mac be due to their inherent biological properties and may also be attributed to the rich presence of hydroxyl groups in myricetin chemical structure [20].…”

Section: Discussionmentioning

confidence: 99%

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In vitro: The Modulating Effect of Myricetin on the Atherosclerosis Related Processes in THP1 Macrophages

Almassabi

Huwait²,

Almowallad

et al. 2021

JPRI

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Aims: To assess the anti-atherosclerotic effects of Myricetin (pharmaceutical) in human THP-1 macrophages following IFN-γ or MCP-1 stimulation. Study Design: The protective effects of myricetin against atherosclerosis was evaluated using the humanTHP-1 macrophages and studying the following parameters namely, cell viability, cell proliferation, cell migration, inflammation related gene expression and cholesterol efflux in vitro. Place and Duration of Study: THP-1 cell line: Department of biochemistry (faculty of science), Cell Culture Unit, Experimental Biochemistry Unit (King Fahad Medical Research Centre), King Abdul Aziz University, between September 2019 and September 2020. Methodology: The THP-1 cell lines were differentiated into macrophages by incubation with PMA (160 nM) for 24 hours. The viability percentage was determined using Pierce LDH cytotoxicity assay kit, the percentage change in macrophages proliferation was evaluated by crystal violet dye, the RNA was extracted then the cDNA was synthesized and the quantitative real time polymerase chain reaction (qRT-PCR) was done for inflammation-related genes, ICAM-1 and MCP-1. The percentage of monocyte migration and cholesterol efflux were also calculated. Results: Cytotoxicity assays demonstrated no significant toxicity with myricetin at 25μM and 50 μM concentrations on THP-1 macrophages. The quantitative real-time RT-PCR (qRT-PCR) demonstrated a significant increase in interferon gamma (IFN-γ) mediated expression of both intercellular adhesion molecule (ICAM-1) and monocyte chemo-attractant protein-1 (MCP-1) by 2.1 and 7.1 fold respectively, compared to the control. Treatment with myricetin (25 μM and 50 μM) significantly inhibited the IFN-γ induced overexpression of ICAM-1 by 42.86% & 71.34% and MCP-1 by 53.52% & 87.32% respectively. Myricetin (25 μM) significantly reduced the migration of monocytes by 33.66% compared to MCP-1. The cholesterol efflux from THP-1 macrophages treated with myricetin was significantly increased by 47% and 57% in the absence and presence of IFN-γ, respectively compared to the control. Conclusion: Myricetin has anti-inflammatory effects and supports cholesterol efflux, which can help in prevention of atherosclerosis. Furthermore, myricetin did not exhibit any cytotoxic effects and therefore is a safe phytochemical which can complement conventional therapeutics.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

In vitro: The Modulating Effect of Myricetin on the Atherosclerosis Related Processes in THP1 Macrophages

Almassabi

Huwait²,

Almowallad

et al. 2021

JPRI

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“…Other flavonoids and their anti-inflammatory and cancer-prevention properties are summarized in Table 1. [190] Paw Edema Mice and THP-1 (together) Inhibits Cox-2; STAT3 and NF-κB Inhibit inflammation [191] Brain Injury and neuroinflammation Rats Inhibits STAT3 and NF-κB p65 Inhibit inflammation [189] Fisetin Hypoxia NCI-H157 Inhibit HIF 1-α and STAT3 Prevents hypoxia [192] Hydrogen peroxide-induced oxidative stress HaCaT Inhibits iNOS, Cox-2, IL-1β, IL-6, and TNF-α Inhibit inflammation [193] Diabetic cardiomyopathy Rats Restores SOD and CAT; inhibits IL-1β, IL-6, and TNF-α; inhibits caspase-3, Bax, and Bax/Bcl-2 ratio Inhibit inflammation and apoptosis [194] Myricetin LPS-induced Inflammation Mice and RAW 264.7 Inhibits NF-κB p65 and AKT activation Inhibit inflammation [195] Colonic…”

Section: Daidzeinmentioning

confidence: 99%

Dietary Flavonoids in p53—Mediated Immune Dysfunctions Linking to Cancer Prevention

et al. 2020

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The p53 protein plays a central role in mediating immune functioning and determines the fate of the cells. Its role as a tumor suppressor, and in transcriptional regulation and cytokine activity under stress conditions, is well defined. The wild type (WT) p53 functions as a guardian for the genome, while the mutant p53 has oncogenic roles. One of the ways that p53 combats carcinogenesis is by reducing inflammation. WT p53 functions as an anti-inflammatory molecule via cross-talk activity with multiple immunological pathways, such as the major histocompatibility complex I (MHCI) associated pathway, toll-like receptors (TLRs), and immune checkpoints. Due to the multifarious roles of p53 in cancer, it is a potent target for cancer immunotherapy. Plant flavonoids have been gaining recognition over the last two decades to use as a potential therapeutic regimen in ameliorating diseases. Recent studies have shown the ability of flavonoids to suppress chronic inflammation, specifically by modulating p53 responses. Further, the anti-oxidant Keap1/Nrf2/ARE pathway could play a crucial role in mitigating oxidative stress, leading to a reduction of chronic inflammation linked to the prevention of cancer. This review aims to discuss the pharmacological properties of plant flavonoids in response to various oxidative stresses and immune dysfunctions and analyzes the cross-talk between flavonoid-rich dietary intake for potential disease prevention.

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“…The health benefits of Myr have been thoroughly investigated in over the last decade. Currently, Myr is known display the following biological properties: antioxidant (8), antimicrobial (9), antiviral (10), anti-inflammatory (11), anti-tumor (12), analgesic (13), hepatoprotective (14), hypoglycemic (15), hypolipidemic (16), cardioprotective (17), and neurological damage inhibition (18). Recently, the cardioprotective role of Myr has attracted attention from the research community due to its potential clinical impact (Fig.…”

Section: Biological Sources and Functions Of Myricetinmentioning

confidence: 99%

The Protective Effects of Myricetin against Cardiovascular Disease

Wang

Yang³

et al. 2019

J Nutr Sci Vitaminol

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Cardiovascular disease (CVD) is the leading cause of death globally, except Africa, and poses a severe health burden worldwide. Both in vitro and in vivo studies have demonstrated the protective effects of myricetin for preventing CVD. For this review, we have assessed the literature from 2009 to 2019 at home and abroad to uncover the protective roles of myricetin for preventing CVD. Myricetin exhibits cardioprotective, anti-hypertensive, anti-atherosclerotic, anti-hyperglycemic, and anti-hyperlipidemic effects. In addition, myricetin may alleviate some of the complications caused by adult-onset diabetes. The combined functions of myricetin allow for the prevention of CVD. This review describes the possible therapeutic benefits of myricetin, along with its potential mechanisms of action, to support the clinical use of the myricetin for the prevention of CVD.

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Myricetin Attenuates LPS-induced Inflammation in RAW 264.7 Macrophages and Mouse Models

Abstract: Background: Myricetin has been demonstrated to inhibit inflammation in a variety of diseases, but little

Cited by 13 publications

References 31 publications

In vitro: The Modulating Effect of Myricetin on the Atherosclerosis Related Processes in THP1 Macrophages

In vitro: The Modulating Effect of Myricetin on the Atherosclerosis Related Processes in THP1 Macrophages

Dietary Flavonoids in p53—Mediated Immune Dysfunctions Linking to Cancer Prevention

The Protective Effects of Myricetin against Cardiovascular Disease

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