Myroides guanonis sp. nov., isolated from prehistoric paintings

Abstract

A novel psychrotolerant, strictly aerobic, non-motile, rod-shaped bacterial strain, designated IM13 T , was isolated from a sample taken from prehistoric guano paintings in Magura Cave, northwest Bulgaria and subjected to a polyphasic taxonomic study. Strain IM13 T formed yellow colonies on LB agar plates and was Gram-staining-negative, heterotrophic and alkalitolerant. It grew optimally at pH 7.5 and 30 6C in the absence of NaCl. Phylogenetic analysis of the whole 16S rRNA gene revealed that strain IM13 T branched with representatives of the genus Myroides with sequence similarity of 93-94 % with other species of the genus. The novel isolate contained iso-C 15 : 0 (49.1 %), iso-C 17 : 1 v9c (18.2 %) and iso-C 17 : 0 3-OH (14.0 %) as dominant fatty acids. The DNA G+C content of strain IM13 T was 33.5 mol%. Based on phylogenetic inference and phenotypic characteristics, it was concluded that strain IM13 T represents a novel species of the genus Myroides, for which the name Myroides guanonis sp. nov. is proposed. The type strain is IM13 T (5DSM 26542 T 5NBIMCC 8736 T ).

Association ofMyroides odoratimimusin immunocompromized piglets with post weaning multisystemic wasting syndrome

“…Despite the low pathogenicity potential, management of Myroides infection is difficult owing to high resistant towards most of the available antibiotics (Licker et al 2018). Pathogenicity of other species of the genus Myroides namely, M. pelagicus, M. profundi, M. marinus, M. phaeus, M. guanonis, M. xuanwuensis, M. injenensis and M. indicus for humans is uncertain (Yoon et al 2006;Zhang et al 2008;Cho et al 2011;Yan et al 2012;Tomova et al 2013;Zhang et al 2014;Paek et al 2015;Ram et al 2015).…”

Myroides indicus sp. nov., isolated from garden soil

“…The genus Myroides is a member of the family Flavobacteriaceae (Bernardet et al, 2002). At the time of writing this manuscript, the genus Myroides comprises species isolated from different habitats, including Myroides odoratus and Myroides odoratimimus from clinical samples (Holmes et al, 1977;Vancanneyt et al, 1996), Myroides pelagicus and Myroides marinus from seawater (Yoon et al, 2006;Cho et al, 2011), Myroides profundi from deep-sea sediment (Zhang et al, 2008), Myroides phaeus from human saliva (Yan et al, 2012), Myroides guanonis from a prehistoric guano painting (Tomova et al, 2013), Myroides xuanwuensis from forest soil (Zhang et al, 2014), 'Myroides gitamensis' from slaughter house soil (Talluri et al, 2014) and Myroides injenensis from human urine (Paek et al, 2015). The range of habitats shows the wide distribution of strains representing the genus.…”

“…The kit includes a standardized colorimetric identification system for 35 different carbohydrates based on the principle of pH change due to metabolic activity during bacterial growth. The kit results were confirmed manually for the following 15 different carbohydrates: sucrose, trehalose, maltose, D-xylose, raffinose, diethylaminoethyl cellulose, cellobiose, mannitol, glucose, D-fructose, D-glucose, lactose monohydrate, D-sorbitol, D-galactose and melibiose using a minimal salts medium (g l 21 ); (NH 4 ) 2 HPO 4 , 1.0; KCL, 0.2; MgSO 4 , 0.1; thiamine, 0.0001; and bromothymol blue, 0.00015 (Tomova et al, 2013). The enzyme activities of strain UKS3 T were assessed using API ZYM (bioMérieux) according to manufacturer's instructions.…”

“…SYBR green at Table 1. Cellular fatty acid contents of strain UKS3 T and strains of recognized species of the genus Myroides Strains: 1, UKS3 T ; 2, M. marinus KCTC 23023 T (Cho et al, 2011); 3, M. phaeus JCM 17139 (Yan et al, 2012); 4, M. odoratus DSM 2801 T (Holmes et al, 1977;Vancanneyt et al, 1996); 5, M. guanonis IM13 T (Tomova et al, 2013); 6, M. pelagicus SM1 T (Yoon et al, 2006); 7, M. profundi D25 T (Zhang et al, 2008); 8, M. xuanwuensis TH-19 T (Zhang et al, 2014). Data for first three strains are from this study and was obtained after culturing, harvesting of culture from trypticase soy agar at 28 8C for 24 h and analysis of fatty acid methyl esters according the MIDI standard protocol (version 4.0).…”

“…The DNA G+C content of the type strain is 37 mol%. (Cho et al, 2011); 3, M. phaeus MY15 T (Yan et al, 2012); 4, M. odoratus DSM 2801 T (Holmes et al, 1977); 5, M. guanonis IM13 T (Tomova et al, 2013); 6, M. pelagicus SM1 T (Yoon et al, 2006); 7, M. profundi D25 T (Zhang et al, 2008); 8, M. xuanwuensis TH-19 T (Zhang et al, 2014). The first three strains were studied together and data for the remaining strains were taken from previous studies.…”