Myxobolus cerebralis infection patterns in Yellowstone cutthroat trout after natural exposure

Murcia, Silvia; Kerans, Billie L.; MacConnell, Elizabeth; Koel, Todd M.

doi:10.3354/dao071191

Cited by 21 publications

(28 citation statements)

References 29 publications

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“…Nested PCR was used to detect infection presence/absence and thereby identify which fry heads required histological analysis to determine location and severity of pathology. If a sample tested positive for M. cerebralis DNA by PCR, all 5 heads in the sample were microscopically analyzed using standard histological techniques (Humason 1979) as in Murcia et al (2006). We also analyzed a random sub-sample of 30 PCR-negative half heads to verify lack of infection.…”

Section: Methodsmentioning

confidence: 99%

“…Field exposures were conducted as in Murcia et al (2006) but at 3 sites (upper, middle, lower; Fig. 1) in the lower 13 km of Pelican Creek.…”

Section: Methodsmentioning

confidence: 99%

“…We did not record prevalence of skeletal deformities and black tails because these clinical signs were seldom present . Fry heads were removed and half heads prepared for testing by nested polymerase chain reaction (PCR) for Myxobolus cerebralis DNA (Andree et al 1998) in pools of 5 as in Murcia et al (2006). Nested PCR was used to detect infection presence/absence and thereby identify which fry heads required histological analysis to determine location and severity of pathology.…”

Section: Methodsmentioning

confidence: 99%

“…Unlike our prior work , we correlate infection with a set of environmental predictor variables, placing histopathology of this parasitic infection in the wild, natural context. Here, we focus on the 4 most important infection response variables for survival (cranium, lower and upper jaw cartilages, and inflammation; Murcia et al 2006) of cutthroat trout, across multiple sites and exposure times. Our concurrent studies at these particular sites and exposure times in Pelican Creek identified only lineage III Tubifex tubifex (susceptible to M. cerebralis; Baxa et al 2008) and abundance of infected T. tubifex to be relatively uniform (J. Alexander, Montana State University, pers.…”

Section: Introductionmentioning

confidence: 99%

“…Variables were measured in fry after their exposure to the parasite in sentinel cages (1 m height × 0.5 m diameter) in their natural environment. Parasite-free Yellowstone cutthroat fry (4 to 6 wk old) were obtained from the Wyoming Game and Fish Department's broodstock, originating from the Yellowstone River and Clear Creek, a large tributary on the lake's eastern shore.Field exposures were conducted as in Murcia et al (2006) but at 3 sites (upper, middle, lower; Fig. 1) in the lower 13 km of Pelican Creek.…”

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confidence: 99%

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Correlation of environmental attributes with histopathology of native Yellowstone cutthroat trout naturally infected with Myxobolus cerebralis

Murcia

Kerans²,

MacConnell³

et al. 2011

Dis. Aquat. Org.

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Infection by the invasive parasite Myxobolus cerebralis (causing whirling disease in salmonids) is strongly influenced by a stream's physico-chemical characteristics, which might affect host pathology. We examined whether environmental variables of a M. cerebralis-positive tributary to Yellowstone Lake, Yellowstone National Park, USA, correlated with the histopathology of naturally infected native cutthroat trout Oncorhynchus clarkii bouvieri. Host inflammatory response and cranial cartilage lesions were the main correlates with whirling behavior. Canonical correlation analyses showed that the prevalence of trout with severe lesions in the cranial and jaw cartilages was highest in stream sites with a combination of high temperature and low specific conductivity. Our results reveal that environmental components can affect when and where a pathogen resides within the host, and manifestation of disease. Recognition of the synergism among environmental and histopathology factors most conducive to whirling disease will increase our prediction and detection abilities for M. cerebralis in salmonid hosts.KEY WORDS: Whirling disease · Pathogen · Invasion-environment synergism · Canonical correlation · Yellowstone ecosystem Resale or republication not permitted without written consent of the publisherDis Aquat Org 93: [225][226][227][228][229][230][231][232][233][234] 2011 Hydrothermal and high elevation streams are examples of systems where the physical and chemical environments are variable (e.g. severe temperature or pH fluctuations) and can inflict additional stresses on the native biota beyond seasonal changes. In Lake Wabamun in Alberta, Canada, thermal effluents facilitated parasite transmission between hosts throughout the year and increased prevalence of certain parasites (Sankurathri & Holmes 1976). Synergisms between infection by metacercaria and exposure to pesticides and herbicides were reported as the cause of vertebral deformities in frogs (Kiesecker 2002). These are examples of environments where natural (e.g. hydrothermal) and anthropogenic (e.g. pollutant) stressors often have a strong influence on parasite-host interactions and development of diseases.However, synergisms between the effects of environmental stressors and development of disease from parasitic infection in wild fish are seldom examined through a histopathology approach (but see Roubal 1994). Histopathology is key to identifying target organs of parasitic infection and pathogens' mode of action at the biochemical level of organization (severity of disease), which may affect ecosystem function at the population level of organization (Schwaiger 2001). Histopathological anomalies in fish, for example, are frequently used as indicators of chemical pollution in marine and fresh water environments (Schwaiger 2001, Wester et al. 2002, Kent et al. 2004, identifying risks of population declines. But we found no examples in the scientific literature of studies on the potential relationships between environmental stress and histopatholog...

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Section: Methodsmentioning

confidence: 99%

“…Field exposures were conducted as in Murcia et al (2006) but at 3 sites (upper, middle, lower; Fig. 1) in the lower 13 km of Pelican Creek.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Correlation of environmental attributes with histopathology of native Yellowstone cutthroat trout naturally infected with Myxobolus cerebralis

Murcia

Kerans²,

MacConnell³

et al. 2011

Dis. Aquat. Org.

Self Cite

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Discovery of mineralization in the caudal vertebrae of perch (Perca fluviatilis L.): A potential new tool for environmental impact assessment

Karlsson,

Delling,

Viktor

et al. 2024

Aquaculture Fish & Fisheries

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The frequency of skeletal deformations in fish is a common biomarker when investigating the environmental response to effluents of various origins. This study discovered a new biomarker: elevated mineralization levels of the vertebrae in the caudal region of perch Perca fluviatilis. The study was conducted in northern Sweden in lakes receiving discharges from an iron ore mine and adjacent reference lakes. The field observation was followed up with an egg‐hatching experiment showing a similar lake‐specific pattern in hatching and deformation frequencies in larvae.

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Yellowstone Cutthroat Trout Recovery in Yellowstone Lake: Complex Interactions Among Invasive Species Suppression, Disease, and Climate Change

Glassic,

Chagaris,

Guy

et al. 2023

Fisheries

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In Yellowstone Lake, Wyoming, the largest inland population of nonhybridized Yellowstone Cutthroat Trout Oncorhynchus clarkii bouvieri, hereafter Cutthroat Trout, declined throughout the 2000s because of predation from invasive Lake Trout Salvelinus namaycush, drought, and whirling disease Myxobolus cerebralis. To maintain ecosystem function and conserve Cutthroat Trout, a Lake Trout gill netting suppression program was established in 1995, decreasing Lake Trout abundance and biomass. Yet, the response of Cutthroat Trout to varying Lake Trout suppression levels, collectively with the influence of disease and climate, is unknown. We developed an ecosystem model (calibrated to historical data) to forecast (2020–2050) whether Cutthroat Trout would achieve recovery benchmarks given disease, varying suppression effort, and climate change. Lake Trout suppression influenced Cutthroat Trout recovery; current suppression effort levels resulted in Cutthroat Trout recovering from historical lows in the early 2000s. However, Cutthroat Trout did not achieve conservation benchmarks when incorporating the influence of disease and climate. Therefore, the National Park Service intends to incorporate age‐specific abundance, spawner biomass, or both in conservation benchmarks to provide better indication of how management actions and environmental conditions influence Cutthroat Trout. Our results illustrate how complex interactions within an ecosystem must be simultaneously considered to establish and achieve realistic benchmarks for species of conservation concern.

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Myxobolus cerebralis infection patterns in Yellowstone cutthroat trout after natural exposure

Cited by 21 publications

References 29 publications

Correlation of environmental attributes with histopathology of native Yellowstone cutthroat trout naturally infected with Myxobolus cerebralis

Correlation of environmental attributes with histopathology of native Yellowstone cutthroat trout naturally infected with Myxobolus cerebralis

Discovery of mineralization in the caudal vertebrae of perch (Perca fluviatilis L.): A potential new tool for environmental impact assessment

Yellowstone Cutthroat Trout Recovery in Yellowstone Lake: Complex Interactions Among Invasive Species Suppression, Disease, and Climate Change

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