Myxococcus xanthus R31 Suppresses Tomato Bacterial Wilt by Inhibiting the Pathogen Ralstonia solanacearum With Secreted Proteins

Dong, Honghong; Xu, Xin; Gao, Ruixiang; Li, Yueqiu; Li, Anzhang; Yao, Qing; Zhu, Honghui

doi:10.3389/fmicb.2021.801091

Cited by 20 publications

(15 citation statements)

References 53 publications

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“…A higher relative abundance of K05998 (pseudomonalisin [EC:3.4.21.100], peptidases, and inhibitors) was found in RH. Peptidases have been reported to be potential biocontrol factors participating in the predation of Myxococcus xanthus on R. solanacearum (Dong et al, 2022 ; Figure 6B ). An important differential pathway worth noting is “Quorum sensing,” which was found to be significantly enriched in the diseased rhizosphere ( Figure 6C ).…”

Section: Discussionmentioning

confidence: 99%

Diseased-induced multifaceted variations in community assembly and functions of plant-associated microbiomes

Kuang

Wang

et al. 2023

Front. Microbiol.

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Plant-associated microorganisms are believed to be part of the so-called extended plant phenotypes, affecting plant growth and health. Understanding how plant-associated microorganisms respond to pathogen invasion is crucial to controlling plant diseases through microbiome manipulation. In this study, healthy and diseased (bacterial wilt disease, BWD) tomato (Solanum lycopersicum L.) plants were harvested, and variations in the rhizosphere and root endosphere microbial communities were subsequently investigated using amplicon and shotgun metagenome sequencing. BWD led to a significant increase in rhizosphere bacterial diversity in the rhizosphere but reduced bacterial diversity in the root endosphere. The ecological null model indicated that BWD enhanced the bacterial deterministic processes in both the rhizosphere and root endosphere. Network analysis showed that microbial co-occurrence complexity was increased in BWD-infected plants. Moreover, higher universal ecological dynamics of microbial communities were observed in the diseased rhizosphere. Metagenomic analysis revealed the enrichment of more functional gene pathways in the infected rhizosphere. More importantly, when tomato plants were infected with BWD, some plant-harmful pathways such as quorum sensing were significantly enriched, while some plant-beneficial pathways such as streptomycin biosynthesis were depleted. These findings broaden the understanding of plant–microbiome interactions and provide new clues to the underlying mechanism behind the interaction between the plant microbiome and BWD.

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Section: Discussionmentioning

confidence: 99%

Diseased-induced multifaceted variations in community assembly and functions of plant-associated microbiomes

Kuang

Wang

et al. 2023

Front. Microbiol.

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“…A predation assay was performed on TPM plates (10 mM Tris-HCl (pH 7.6), 1 mM KPO 4 pH 7.6, 8 mM MgSO 4 , 1.5% agar) using the colony-induced predation method as described [ 36 , 37 ]. Overnight cultures of P. aeruginosa PAO1 were resuspended in an LB medium and cultured to the mid-exponential phase.…”

Section: Methodsmentioning

confidence: 99%

A Disturbed Siderophore Transport Inhibits Myxobacterial Predation

Dong

Zhang

et al. 2022

Cells

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Background: Understanding the intrinsic mechanisms of bacterial competition is a fundamental question. Iron is an essential trace nutrient that bacteria compete for. The most prevalent manner for iron scavenging is through the secretion of siderophores. Although tremendous efforts have focused on elucidating the molecular mechanisms of siderophores biosynthesis, export, uptake, and regulation of siderophores, the ecological aspects of siderophore-mediated competition are not well understood. Methods: We performed predation and bacterial competition assays to investigate the function of siderophore transport on myxobacterial predation. Results: Deletion of msuB, which encodes an iron chelate uptake ABC transporter family permease subunit, led to a reduction in myxobacterial predation and intracellular iron, but iron deficiency was not the predominant reason for the decrease in the predation ability of the ∆msuB mutant. We further confirmed that obstruction of siderophore transport decreased myxobacterial predation by investigating the function of a non-ribosomal peptide synthetase for siderophore biosynthesis, a TonB-dependent receptor, and a siderophore binding protein in M. xanthus. Our results showed that the obstruction of siderophores transport decreased myxobacterial predation ability through the downregulation of lytic enzyme genes, especially outer membrane vesicle (OMV)-specific proteins. Conclusions: This work provides insight into the mechanism of siderophore-mediated competition in myxobacteria.

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“…It was later found that myxobacteria could also be used in the control of plant bacterial diseases with remarkable results. Myxococcus xanthus R31 showed a good biological control effect on tomato bacterial wilt [9]. Moreover, the secondary metabolites of myxobacteria cannot be ignored to inhibit plant diseases, which play an important role in agricultural applications.…”

Section: In Agriculturementioning

confidence: 99%

Isolation identification and application of myxobacteria

Bai

2024

Third International Conference on Biological Engineering and Medical Science (ICBioMed2023)

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Myxobacteria is a kind of gram-negative bacteria. As higher prokaryotes, myxobacteria is widely distributed, and it can glide and prey on other bacteria. Myxobacteria have many species and can live in various extreme environments. Due to its special predation characteristics and abundant secondary metabolites, more and more researchers have paid attention to it in recent years. According to these characteristics, myxobacteria have been widely used in agricultural disease prevention and control, medical disease research, food safety and preservation, environmental management and protection, etc. However, because the current technical means are not enough to excavate all kinds of myxobacteria species, the research on their separation and classification is still slow. This thesis summarizes the methods and status quo of myxobacteria isolation and identification in recent years, research progress on predation and secondary metabolites of myxobacteria in different disciplines and fields. On this basis, the bottleneck of myxobacteria research and its future prospects are put forward.

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Myxococcus xanthus R31 Suppresses Tomato Bacterial Wilt by Inhibiting the Pathogen Ralstonia solanacearum With Secreted Proteins

Cited by 20 publications

References 53 publications

Diseased-induced multifaceted variations in community assembly and functions of plant-associated microbiomes

Diseased-induced multifaceted variations in community assembly and functions of plant-associated microbiomes

A Disturbed Siderophore Transport Inhibits Myxobacterial Predation

Isolation identification and application of myxobacteria

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