N-(1-Naphthyl)ethylenediamine dihydrochloride as a new reagent for nanomole quantification of sugars on thin-layer plates by a mathematical calibration process

Bounias, M.

doi:10.1016/0003-2697(80)90523-0

Cited by 150 publications

(62 citation statements)

References 7 publications

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“…Thin-layer chromatography (TLC) was carried out using silica gel 60 plates with three solvent ascents of acetonitrile-water 4:1 (v/v). Sugars were made visible using N-(1-naphthyl) ethylenediamine dihydrochloride in 3% (v/v) sulfuric acid in methanol [20]. High-performance liquid chromatography (HPLC) was performed using a Waters HPLC system with refractive index detector, fitted with a Regis Spherisorb S5NH column, 5 µm particle size, 4.6 mm × 25 cm, eluted with acetonitrile-water 4:1 (v/v) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Production of isomelezitose from sucrose by engineered glucansucrases

Côté

Dunlap

Vermillion

et al. 2017

Amylase

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Certain lactic acid bacteria produce glycosyltransferases known as glucansucrases, which synthesize α-D-glucans via glucosyl transfer from sucrose. We recently reported on the formation of the unusual trisaccharide isomelezitose in low yields by a variety of glucansucrases. Isomelezitose is a rare non-reducing trisaccharide, with the structure α-d-glucopyranosyl- (1→6)-β-d-fructofuranosyl-(2↔1)-α-d-glucopyranoside. In this work, we describe the synthesis of isomelezitose in high yields by variants of glucansucrases engineered to contain a single point mutation at a key leucine residue involved in acceptor substrate binding. Some variants produce isomelezitose in yields up to 57%. This method is amenable to large-scale production of isomelezitose for food, industrial and biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Production of isomelezitose from sucrose by engineered glucansucrases

Côté

Dunlap

Vermillion

et al. 2017

Amylase

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“…The solvent systems used included n-propanol: pyridine: water-5:3:2 and n-butanol: pyridine: water-8:4:3. Visualizations of the spots was done by spraying with N-(1-naphthyl)-ethylenediaminedihydrochloride in methanol containing 3% H 2 SO 4 (Bounias, 1980).Quantification of trehalose was done using a standard optical density curve ate 546 nm against sugar concentration varying from 300 ng to 1 mg/ml).…”

Section: Hptlc Methodmentioning

confidence: 99%

Analysis of Carbohydrates in Newly Developed Liquid State Rabies Vaccine

Jagannathan¹,

Vijayakumar²

2010

JMBT

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“…3) as acetyl acceptors, were analyzed by TLC on aluminium sheets coated with Silica gel 60 (Merck, Germany), in the acetonitrile/water 9:1 (v/v) solvent system. Sugars were detected on dried chromatograms, using the N-(1-naphthyl)ethylenediamine dihydrochloride reagent, according to Bounias [19]. The chromatograms treated with the detection reagent were heated at 110°C in an oven and cooled prior to analysis.…”

Section: Analytical Proceduresmentioning

confidence: 99%

Carbohydrate esterases of family 2 are 6‐O‐deacetylases

Kyriakopoulos¹,

Biely²,

Hirsch³

et al. 2009

FEBS Letters

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a b s t r a c tThree acetyl esterases (AcEs) from the saprophytic bacteria Cellvibrio japonicus and Clostridium thermocellum, members of the carbohydrate esterase (CE) family 2, were tested for their activity against a series of model substrates including partially acetylated gluco-, manno-and xylopyranosides. All three enzymes showed a strong preference for deacetylation of the 6-position in aldohexoses. This regioselectivity is different from that of typical acetylxylan esterases (AcXEs). In aqueous medium saturated with vinyl acetate, the CE-2 enzymes catalyzed transacetylation to the same position, i.e., to the primary hydroxyl group of mono-and disaccharides. Xylose and xylooligosaccharides did not serve as acetyl group acceptors, therefore the CE-2 enzymes appear to be 6-Odeacetylases.

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N-(1-Naphthyl)ethylenediamine dihydrochloride as a new reagent for nanomole quantification of sugars on thin-layer plates by a mathematical calibration process

Cited by 150 publications

References 7 publications

Production of isomelezitose from sucrose by engineered glucansucrases

Production of isomelezitose from sucrose by engineered glucansucrases

Analysis of Carbohydrates in Newly Developed Liquid State Rabies Vaccine

Carbohydrate esterases of family 2 are 6‐O‐deacetylases

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