N-Acetyl Cysteine-Mediated Improvements in Dental Restorative Material Biocompatibility

Matsuura, Takanori; Komatsu, Keiji; Ogawa, Takahiro

doi:10.3390/ijms232415869

Cited by 7 publications

(6 citation statements)

References 58 publications

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“…Polymerization initiators such as benzoyl peroxide (BPO) and camphorquinone (CQ) compromise cell viability [ 47 , 59 , 60 , 61 ]. BPO, a major initiator for self-curing acrylic resin, is broken down during polymerization to release radicals that injure surrounding cells [ 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

“…Further experiments using keratinocytes and the derivatives of epithelial cells were desired. Future studies other than those already mentioned may include the development of materials with high cytocompatibility properties (i.e., N-acetyl cysteine and tri-n-butyl borane) to improve reduction–oxidation systems and reduce radical production [ 53 , 61 , 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

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Disparity in the Influence of Implant Provisional Materials on Human Gingival Fibroblasts with Different Phases of Cell Settlement: An In Vitro Study

Matsuura,

Stavrou,

Komatsu

et al. 2023

IJMS

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The development of healthy peri-implant soft tissues is critical to achieving the esthetic and biological success of implant restorations throughout all stages of healing and tissue maturation, starting with provisionalization. The purpose of this study was to investigate the effects of eight different implant provisional materials on human gingival fibroblasts at various stages of cell settlement by examining initial cell attachment, growth, and function. Eight different specimens—bis-acrylic 1 and 2, flowable and bulk–fill composites, self-curing acrylic 1 and 2, milled acrylic, and titanium (Ti) alloy as a control—were fabricated in rectangular plates (n = 3). The condition of human gingival fibroblasts was divided into two groups: those in direct contact with test materials (contact experiment) and those in close proximity to test materials (proximity experiment). The proximity experiment was further divided into three phases: pre-settlement, early settlement, and late settlement. A cell culture insert containing each test plate was placed into a well where the cells were pre-cultured. The number of attached cells, cell proliferation, resistance to detachment, and collagen production were evaluated. In the contact experiment, bis-acrylics and composites showed detrimental effects on cells. The number of cells attached to milled acrylic and self-curing acrylic was relatively high, being approximately 70% and 20–30%, respectively, of that on Ti alloy. There was a significant difference between self-curing acrylic 1 and 2, even with the same curing modality. The cell retention ability also varied considerably among the materials. Although the detrimental effects were mitigated in the proximity experiment compared to the contact experiment, adverse effects on cell growth and collagen production remained significant during all phases of cell settlement for bis-acrylics and flowable composite. Specifically, the early settlement phase was not sufficient to significantly mitigate the material cytotoxicity. The flowable composite was consistently more cytotoxic than the bulk–fill composite. The harmful effects of the provisional materials on gingival fibroblasts vary considerably depending on the curing modality and compositions. Pre-settlement of cells mitigated the harmful effects, implying the susceptibility to material toxicity varies depending on the progress of wound healing and tissue condition. However, cell pre-settlement was not sufficient to fully restore the fibroblastic function to the normal level. Particularly, the adverse effects of bis-acrylics and flowable composite remained significant. Milled and self-curing acrylic exhibited excellent and acceptable biocompatibility, respectively, compared to other materials.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Disparity in the Influence of Implant Provisional Materials on Human Gingival Fibroblasts with Different Phases of Cell Settlement: An In Vitro Study

Matsuura,

Stavrou,

Komatsu

et al. 2023

IJMS

Self Cite

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show abstract

“…Primary human oral fibroblasts (ScienCell Research Laboratories, Carlsbad, CA, USA) were cultured, as reported elsewhere [94][95][96], in a company-recommended medium supplemented with 5% fetal bovine serum (FBS), 1% fibroblast growth supplement-2 (ScienCell Research Laboratories), and 1% penicillin/streptomycin solution. At 80% confluence, the cells were detached using 0.05% trypsin-EDTA solution and seeded onto each test zirconia specimen placed in a well (20 mm diameter) of 12-well culture plates at a density of 4 × 10 4 cells/well.…”

Section: Cell Culturementioning

confidence: 99%

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

Suzumura,

Matsuura,

Komatsu

et al. 2023

Cells

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Soft tissue adhesion and sealing around dental and maxillofacial implants, related prosthetic components, and crowns are a clinical imperative to prevent adverse outcomes of periodontitis and periimplantitis. Zirconia is often used to fabricate implant components and crowns. Here, we hypothesized that UV treatment of zirconia would induce unique behaviors in fibroblasts that favor the establishment of a soft tissue seal. Human oral fibroblasts were cultured on zirconia specimens to confluency before placing a second zirconia specimen (either untreated or treated with one minute of 172 nm vacuum UV (VUV) light) next to the first specimen separated by a gap of 150 µm. After seven days of culture, fibroblasts only transmigrated onto VUV-treated zirconia, forming a 2.36 mm volume zone and 5.30 mm leading edge. Cells migrating on VUV-treated zirconia were enlarged, with robust formation of multidirectional cytoplastic projections, even on day seven. Fibroblasts were also cultured on horizontally placed and 45° and 60° tilted zirconia specimens, with the latter configurations compromising initial attachment and proliferation. However, VUV treatment of zirconia mitigated the negative impact of tilting, with higher tilt angles increasing the difference in cellular behavior between control and VUV-treated specimens. Fibroblast size, perimeter, and diameter on day seven were greater than on day one exclusively on VUV-treated zirconia. VUV treatment reduced surface elemental carbon and induced superhydrophilicity, confirming the removal of the hydrocarbon pellicle. Similar effects of VUV treatment were observed on glazed zirconia specimens with silica surfaces. One-minute VUV photofunctionalization of zirconia and silica therefore promotes human oral fibroblast attachment and proliferation, especially under challenging culture conditions, and induces specimen-to-specimen transmigration and sustainable photofunctionalization for at least seven days.

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“…Moreover, it was reported that different amino acids are utilized to fabricate chemical caries removal products, such as Caridex and Carisolv [81]. Another application of amino acids was investigated, as it has been reported that novel dental restorative materials and cements were made by incorporating different amino acids [82][83][84][85]. Zilm et al showed that D-amino acids can reduce the bacterial biofilm of Enterococcus faecalis in the presence of other antibacterial agents in an in vitro model [86].…”

Section: Potential Uses Of Ad (Baobab) Extracts In Dentistry Accordin...mentioning

confidence: 99%

The Potential Uses of Baobab Tree's Medicinal Effects in Dentistry: A Literature Review

Abuljadayel

2023

Cureus

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Adansonia digitata (Baobab) tree is an African tree with a long history in traditional medicine. The local inhabitants of Africa have been using the different tree components to treat medical diseases, such as fever, diarrhea, malaria, cough, dysentery, and microbial infections. Recently, the tree gained the attention of scientists due to its medical and pharmaceutical properties and nutritional values, which generated a myriad number of investigations regarding its phytochemical and macro-and micronutrient contents. The fruit pulp is especially rich in vitamin C, pectin, fibers, and minerals such as calcium, magnesium, potassium, phosphorus, zinc, iron, and copper. Additionally, the leaves contain high levels of calcium, while the seeds are considered a good source of protein and fat. Altogether, they contain a variety of polyphenols, fatty acids, and amino acids. The tree extracts possess potent antioxidant, cell-protective, and anti-inflammatory activities. However, no information was found in the literature about the use of Baobab tree products in the dental field. The aim of this review is to discuss the well-documented medical effects and chemical and mineral components of the different Baobab tree parts from a dental point of view to open more areas of research concerning its potential applications in the dental field. Antioxidants and vitamin C are known to help in maintaining healthy periodontal and gingival tissues. They also help in wound healing and alveolar bone integrity. Moreover, phytochemicals and phenolic compounds have been utilized in controlling dental plaque and manufacturing intracanal medications as they manifest antimicrobial and anti-inflammatory activities. Furthermore, calcium and phosphorus incorporation in dental biomaterials is commonly used in vital pulp therapy and repairing bone defects. After reviewing the reported medicinal and pharmaceutical activities of the Baobab tree, it can be inferred that the tree extracts possess potential uses in the dental field, which requires further investigation for validation.

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N-Acetyl Cysteine-Mediated Improvements in Dental Restorative Material Biocompatibility

Cited by 7 publications

References 58 publications

Disparity in the Influence of Implant Provisional Materials on Human Gingival Fibroblasts with Different Phases of Cell Settlement: An In Vitro Study

Disparity in the Influence of Implant Provisional Materials on Human Gingival Fibroblasts with Different Phases of Cell Settlement: An In Vitro Study

Vacuum Ultraviolet (VUV) Light Photofunctionalization to Induce Human Oral Fibroblast Transmigration on Zirconia

The Potential Uses of Baobab Tree's Medicinal Effects in Dentistry: A Literature Review

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