N-acetyl cysteine (NAC)-mediated detoxification and functionalization of poly(methyl methacrylate) bone cement

Tsukimura, Naoki; Yamada, Masahiro; Aita, Hideki; Hori, Norio; Yoshino, Fumihiko; Lee, Masaichi Chang-il; Kimoto, Katsuhiko; Jewett, Anahid; Ogawa, Takahiro

doi:10.1016/j.biomaterials.2009.02.043

Cited by 83 publications

(100 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NAC can directly scavenge ROS and toxic compounds with its functional moiety ). In addition, NAC has the cellular pharmacodynamics to elevate intracellular glutathione levels, as shown in the present study, which indicated that NAC may enhance the anti-oxidant resistance of osteoblasts against oxidative stress from materials (Tsukimura et al, 2009). Furthermore, NAC might modulate cysteine-containing transcriptional factors such as nuclear factor kappa B, which regulates cell survival and differentiation (Paranjpe et al, 2007).…”

Section: Discussionsupporting

confidence: 64%

See 1 more Smart Citation

N-acetyl Cysteine Alleviates Cytotoxicity of Bone Substitute

et al. 2010

View full text Add to dashboard Cite

A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. AbstrAct Lack of cytocompatibility in bone substitutes impairs healing in surrounding bone. Adverse biological events around biomaterials may be associated with oxidative stress. We hypothesized that a clinically used inorganic bone substitute is cytotoxic to osteoblasts due to oxidative stress and that N-acetyl cysteine (NAC), an antioxidant amino acid derivative, would detoxify such material. Only 20% of rat calvaria osteoblasts were viable when cultured on commercial deproteinized bovine bone particles for 24 hr, whereas this percentage doubled on bone substitute containing NAC. Intracellular ROS levels markedly increased on and under bone substitutes, which were reduced by prior addition of NAC to materials. NAC restored suppressed alkaline phosphatase activity in the bone substitute. Proinflammatory cytokine levels from human osteoblasts on the bone substitute decreased by one-third or more with addition of NAC. NAC alleviated cytotoxicity of the bone substitute to osteoblastic viability and function, implying enhanced bone regeneration around NAC-treated inorganic biomaterials.

show abstract

Section: Discussionsupporting

confidence: 64%

“…Indeed, NAC detoxified PMMA-based bone cement, resulting in remarkably enhanced bone-contact area, surrounding bone volume, and biomechanical strength at the bone-material interface as compared with the original cement alone (Tsukimura et al, 2009 …”

Section: J Dent Res 89(4) 2010mentioning

confidence: 99%

N-acetyl Cysteine Alleviates Cytotoxicity of Bone Substitute

et al. 2010

View full text Add to dashboard Cite

show abstract

“…NAC, a cysteine analogue drug with many therapeutic applications, has a protective role in controlling oxidative stress against many cells including osteoblasts, and stimulates osteoblastic differentiation of mouse calvarial cells (8,14,47,62). Other studies report that NAC inhibits oxidative stress induced apoptosis of osteoblastic cells, and this is mediated by GSH (63,64), moreover, NAC prevents osteoclast formation, NF-κB activation and TNF-α expression involved in osteoclast activation (49). Indeed, GSH is involved in osteoblast and osteoclast differentiation as well as in osteoporosis and other bone diseases (14,17,65).…”

Section: Antioxidants In Bone Remodeling and In Bone Lossmentioning

confidence: 99%

Oxidative stress in bone remodeling: role of antioxidants

Domazetovic

Marcucci

Iantomasi

et al. 2017

ccmbm

568

524

View full text Add to dashboard Cite

SummaryROS are highly reactive molecules which consist of a number of diverse chemical species, including radical and non-radical oxygen species. Oxidative stress occurs as a result of an overproduction of ROS not balanced by an adequate level of antioxidants. The natural antioxidants are: thiol compounds among which GSH is the most representative, and non-thiol compounds such as polyphenols, vitamins and also various enzymes. Many diseases have been linked to oxidative stress including bone diseases among which one of the most important is the osteoporosis. The redox state changes are also related to the bone remodeling process which allows the continuous bone regeneration through the coordinated action of bone cells: osteoclasts, osteoblasts and osteocytes. Changes in ROS and/or antioxidant systems seem to be involved in the pathogenesis of bone loss. ROS induce the apoptosis of osteoblasts and osteocytes, and this favours osteoclastogenesis and inhibits the mineralization and osteogenesis. Excessive osteocyte apoptosis correlates with oxidative stress causing an imbalance in favor of osteoclastogenesis which leads to increased turnover of bone remodeling and bone loss. Antioxidants either directly or by counteracting the action of oxidants contribute to activate the differentiation of osteoblasts, mineralization process and the reduction of osteoclast activity. In fact, a marked decrease in plasma antioxidants was found in aged or osteoporotic women. Some evidence shows a link among nutrients, antioxidant intake and bone health. Recent data demonstrate the antioxidant properties of various nutrients and their influence on bone metabolism. Polyphenols and anthocyanins are the most abundant antioxidants in the diet, and nutritional approaches to antioxidant strategies, in animals or selected groups of patients with osteoporosis or inflammatory bone diseases, suggest the antioxidant use in anti-resorptive therapies for the treatment and prevention of bone loss.

show abstract

“…[24] Remarkably, Aita et al [25] showed that the NAC-loaded bone cement prevents osteoblastic cytotoxicity. Because NAC appears to not cytotoxic, it has been used in concentrations as high as 6 g. There is a practical upper limit to the amount of NAC that can be added to bone cement, [26] but the aim of this study was not to examine the mechanical stability of NAC-loaded bone cement.…”

Section: Discussionmentioning

confidence: 99%