N-Acetylcysteine in Mechanically Ventilated Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome: The Effect of Intravenous Dose on Oxidative Damage and Inflammation

M, Kolomazník; Mikolka, Pavol; Hanusrichterova, Juliana; Kosutova, Petra; Matasová, Katarína; Mokrá, Daniela; Čalkovská, Andrea

doi:10.3390/biomedicines9121885

Cited by 6 publications

(5 citation statements)

References 49 publications

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“…However, it failed to protect the rats against developing pulmonary edema and lethality. A study on a mechanically ventilated rat with lipopolysaccharide-induced ALI reported that a high dose of NAC reduced oxidative damage and inflammatory cell in the histology of the lungs [14]. Other studies also reported that NAC treatment protected against lipopolysaccharide-induced ALI [16].…”

Section: Discussionmentioning

confidence: 99%

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The Role of Antioxidant Agent (N-Acetylcysteine) in Oleic Acid-Induced Acute Lung Injury in a Rat Model

Kumar¹,

Bhagat²,

Pandey³

et al. 2022

Cureus

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ContextReactive oxygen species (ROS) produced by inflammatory cells play a major role in mediating lung injury in sepsis or hyperoxic lung injury. AimsN-Acetylcysteine (NAC), an antioxidant, was examined in this research to see whether it helps prevent acute lung injury (ALI). Materials and methodsExperiments were performed on Charles-Foster strain healthy male adult albino rats. All the animals were randomly divided into one control and two experimental groups. In control/group I, saline was administered, and cardiorespiratory parameters were recorded. Oleic acid (OA) was administered in group II to produce ALI. In group III, OA was administered to NAC-pretreated rats, and cardiorespiratory parameters were recorded to observe the effect of NAC on ALI. This study used analysis of variance (ANOVA) with two factors and a post hoc test (multiple comparisons -least significant difference (LSD) test) for statical analysis. For determining survival time, the Mantel-Cox test and Kaplan-Meier survival curves were used. A P value < 0.05 was considered significant. ResultsRespiratory arrest, pulmonary edema, and reduced partial pressure of oxygen (PaO 2 )/fraction of inspired oxygen (FiO 2 ) ratio were all indications of OA-induced ALI in rats. The animals in the NAC + OA group had better respiratory and cardiac statistics than those in the OA alone group, and their survival duration was extended. However, NAC pretreatment could not protect the animals against the development of pulmonary edema. ConclusionsThese observations indicate that NAC (an antioxidant agent) protected rats against ALI in the initial phase and prolonged the survival time but failed to prevent the development of pulmonary edema.

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Section: Discussionmentioning

confidence: 99%

“…In group III (NAC + OA) (n = 6), after initial recordings of RR, HR, and BP, N-acetylcysteine (163 mg/kg IP) was injected to record the response of RR, HR, and BP [14]. Five minutes after NAC pretreatment, OA (75 µl IV) was administered in rats, and the response was recorded.…”

Section: Experimental Protocolmentioning

confidence: 99%

The Role of Antioxidant Agent (N-Acetylcysteine) in Oleic Acid-Induced Acute Lung Injury in a Rat Model

Kumar¹,

Bhagat²,

Pandey³

et al. 2022

Cureus

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“…In an experimental study, Kolomaznik et al . 18 showed benefits of using NAC in rat model of endotoxemia. It was demonstrated by the reduction of inflammatory markers and oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Effects of N-acetylcysteine on the inflammatory response and bacterial translocation in a model of intestinal obstruction and ischemia in rats

et al. 2022

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Purpose: To evaluate effect of N-acetylcysteine (NAC) associated with Ringer lactate or hypertonic saline in inflammation and bacterial translocation on experimental intestinal obstruction (IO). Methods: Wistar rats was subjected to IO. Six or 24 hours after, rats were subjected to enterectomy and fluid resuscitation: IO, RL (subjected to the same procedures but with fluid resuscitation using Ringer’s lactate solution); RLNAC (added NAC to Ringer’s solution); and HSNAC (surgical procedure + fluid reposition with 7.5% hypertonic saline and NAC). After 24 h, tissues were collected to cytokines, bacterial translocation, and histological assessments. Results: In kidney, interleukin-1beta (IL-1beta) was lower in the groups with fluid resuscitation compared to IO group. The RLNAC showed lower levels compared to the RL. Interleukin-6 (IL-6), interleukin-10 (IL-10), tumor necrosis factor-alpha (TNF-alpha), and ( IFN-gamma ) were lower in the treatment groups than in IO. In lung, IL-1beta and IL-6 were lower in RLNAC compared to IO. IL-10 was lower in RL, RLNAC and HSNAC compared to IO. TNF-alpha was higher in HSNAC compared to both RL and RLNAC. Bacterial translocation was observed in all animals of IO group. In kidneys, inflammation and congestion degrees were lower in HSNAC compared to RL. In lungs, inflammation levels were higher in RLNAC compared with the sham group. Conclusions: The data indicates that NAC associated with RL can promote a decrease in the inflammatory process in the kidneys and lungs in rats, following intestinal obstruction and ischemia in rats.

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“…50 μL of Saline or tDONs-R9 (60 nM) were intratracheally administrated to ALI model mice at 3 h post LPS stimulation. Meanwhile, NAC, a clinically used drug for broad-spectrum ROS scavenging, as the positive control, − was intravenously injected into ALI model mice at 3 h post LPS stimulation (Figure a). All mice were euthanized, and BALF or the lungs were collected for further analysis at 24 h post LPS stimulation.…”

Section: Therapeutic Potency Of Tdons-r9 In Ali Mouse Modelmentioning

confidence: 99%

Nebulized Inhalation of Peptide-Modified DNA Origami To Alleviate Acute Lung Injury

Wang,

Jiao,

et al. 2024

Nano Lett.

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Acute lung injury (ALI) is a severe inflammatory lung disease, with high mortality rates. Early intervention by reactive oxygen species (ROS) scavengers could reduce ROS accumulation, break the inflammation expansion chain in alveolar macrophages (AMs), and avoid irreversible damage to alveolar epithelial and endothelial cells. Here, we reported cell-penetrating R9 peptide-modified triangular DNA origami nanostructures (tDONs-R9) as a novel nebulizable drug that could reach the deep alveolar regions and exhibit an enhanced uptake preference of macrophages. tDONs-R9 suppressed the expression of pro-inflammatory cytokines and drove polarization toward the anti-inflammatory M2 phenotype in macrophages. In the LPS-induced ALI mouse model, treatment with nebulized tDONs-R9 alleviated the overwhelming ROS, pro-inflammatory cytokines, and neutrophil infiltration in the lungs. Our study demonstrates that tDONs-R9 has the potential for ALI treatment, and the programmable DNA origami nanostructures provide a new drug delivery platform for pulmonary disease treatment with high delivery efficiency and biosecurity.

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N-Acetylcysteine in Mechanically Ventilated Rats with Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome: The Effect of Intravenous Dose on Oxidative Damage and Inflammation

Cited by 6 publications

References 49 publications

The Role of Antioxidant Agent (N-Acetylcysteine) in Oleic Acid-Induced Acute Lung Injury in a Rat Model

The Role of Antioxidant Agent (N-Acetylcysteine) in Oleic Acid-Induced Acute Lung Injury in a Rat Model

Effects of N-acetylcysteine on the inflammatory response and bacterial translocation in a model of intestinal obstruction and ischemia in rats

Nebulized Inhalation of Peptide-Modified DNA Origami To Alleviate Acute Lung Injury

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