N-acetylcysteine prevents stress-induced anxiety behavior in zebrafish

Mocelin, Ricieri; Herrmann, Ana Paula; Marcon, Matheus; Rambo, Cassiano L.; Rohden, Aline; Bevilaqua, Fernanda; Abreu, Murilo S. de; Zanatta, Leïla; Elisabetsky, Elaine; Barcellos, Leonardo José Gil; Lara, Diogo R.; Piato, Ângelo

doi:10.1016/j.pbb.2015.08.006

“…While a growing body of evidence supports the role of glutamate in the anxiety response, there is a clear deficit of approved glutamatergic anxiolytics (Cortese and Phan, ). NAC administration to adult zebrafish prevents stress‐induced anxiety (Mocelin et al, ), which is in line with previous reports of its clinical efficacy in depressed patients (Berk et al, ). In another example of drug repositioning, potential anxiolytic targets were identified using traditional cancer treatments, as cAMP mediated anxiety in the zebrafish via crosstalk of the RAS‐MAPK pathway (Lundegaard et al, ).…”

Section: Zebrafish Models Of Major Cns Disorderssupporting

confidence: 89%

Zebrafish models in neuropsychopharmacology and CNS drug discovery

Khan

¹

,

Collier

²

,

Meshalkina

³

et al. 2017

British J Pharmacology

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Despite the high prevalence of neuropsychiatric disorders, their aetiology and molecular mechanisms remain poorly understood. The zebrafish (Danio rerio) is increasingly utilized as a powerful animal model in neuropharmacology research and in vivo drug screening. Collectively, this makes zebrafish a useful tool for drug discovery and the identification of disordered molecular pathways. Here, we discuss zebrafish models of selected human neuropsychiatric disorders and drug-induced phenotypes. As well as covering a broad range of brain disorders (from anxiety and psychoses to neurodegeneration), we also summarize recent developments in zebrafish genetics and small molecule screening, which markedly enhance the disease modelling and the discovery of novel drug targets.

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“…Chronic ethanol exposure also produces oxidative stress, but not increased nitrite, in the zebrafish brain [23], suggesting that the effect on the nitrergic pathway is specific to withdrawal. Both oxidative stress [24,25] and nitric oxide [26,27] have been implicated in stress-induced anxiogenesis in zebrafish, suggesting that the nitrergic system is a common mediator of the behavioral effects of stressors. EtOH-WD has also been shown, in rodents, to sensitize neurons associated with defensive behavior [28,29].…”

Section: Discussionmentioning

confidence: 99%

NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish

Chaves¹,

Costa²,

Gomes³

et al. 2020

Preprint

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Nitric oxide has been implicated in symptoms of ethanol withdrawal in animal models. Zebrafish have been used as models to study neurobehavioral effects of ethanol (EtOH) withdrawal, but the mechanisms associated with these effects are not yet clear. Adult zebrafish were treated with 1% EtOH for 20 min per day for 8 days, injected with the nitric oxide synthase 2 (NOS-2) inhibitor aminoguanidine (50 mg/kg), and allowed to experience withdrawal (WD) in their hometanks for 7 days. EtOH WD increased anxiety-like behavior in the novel tank test, an effect that was blocked by aminoguanidine. EtOH WD also increased brain levels of nitrite, an effect that was partially blocked by aminoguanidine. These results underline a novel mechanism by which NOS-2 controls anxiety-like responses to ethanol withdrawal, with implications for the mechanistic study of symptoms associated with chronic ethanol abuse.

show abstract

“…Chronic ethanol exposure also produces oxidative stress, but not increased nitrite, in the zebrafish brain [28], suggesting that the effect on the nitrergic pathway is specific to withdrawal. Both oxidative stress [29,30] and nitric oxide [31,32] have been implicated in stress-induced anxiogenesis in zebrafish, suggesting that the nitrergic system is a common mediator of the behavioral effects of stressors. EtOH-WD has also been shown, in rodents, to sensitize neurons associated with defensive behavior [33,34].…”

Section: Discussionmentioning

confidence: 99%

NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish

Chaves¹,

Costa²,

Gomes³

et al. 2020

Preprint

0

View full text Add to dashboard Cite

Nitric oxide has been implicated in symptoms of ethanol withdrawal in animal models. Zebrafish have been used as models to study neurobehavioral effects of ethanol (EtOH) withdrawal, but the mechanisms associated with these effects are not yet clear. Adult zebrafish were treated with 1% EtOH for 20 min per day for 8 days, injected with the nitric oxide synthase 2 (NOS-2) inhibitor aminoguanidine (50 mg/kg), and allowed to experience withdrawal (WD) in their hometanks for 7 days. EtOH WD increased anxiety-like behavior in the novel tank test, an effect that was blocked by aminoguanidine. EtOH WD also increased brain levels of nitrite, an effect that was partially blocked by aminoguanidine. These results underline a novel mechanism by which NOS-2 controls anxiety-like responses to ethanol withdrawal, with implications for the mechanistic study of symptoms associated with chronic ethanol abuse.

show abstract

N-acetylcysteine prevents stress-induced anxiety behavior in zebrafish

Cited by 70 publications

References 49 publications

Zebrafish models in neuropsychopharmacology and CNS drug discovery

Zebrafish models in neuropsychopharmacology and CNS drug discovery

NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish

NOS-2 Participates in the Behavioral Effects of Ethanol Withdrawal in Zebrafish

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