N-acetylcysteine provides protection against the toxicity of dietary T-2 toxin in juvenile Chinese mitten crab (Eriocheir sinensis)

Pan, Jingyu; Wang, Xiaodan; Cai, Xinyu; Lin, Zhideng; Shi, Qingchao; Li, Erchao; Qin, Jian G.; Chen, Liqiao

doi:10.1016/j.aquaculture.2021.736531

Cited by 14 publications

(2 citation statements)

References 83 publications

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“…The immune defense system can be activated by oxidative stress in aquatic animals [106,107]. AKP and ACP play important roles in the immune system, and AKP and ACP in crustaceans participate in the first line of nonspecific immunity [108][109][110]. ACP, as a key compound of lysosomal enzymes, which is one of the important marker enzymes of lysozyme's ability to break down invading organisms, plays a crucial role in the immune system and has been used as a marker of macrophage activation in animal models [111][112][113].…”

Section: Effects Of Cold Stress On Antioxidants Immune Defense and Os...mentioning

confidence: 99%

Effects of Cold Stress on the Hemolymph of the Pacific White Shrimp Penaeus vannamei

Zhu,

Shi,

Zhao

et al. 2024

Fishes

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Temperature is an important factor in the physiological processes of aquatic organisms and can seriously affect several chemical and biological processes in their bodies, including respiratory metabolism, antioxidant capacity, immune capacity, and signal transduction. In this study, physiological, transcriptomic, and metabolomic analyses were used to investigate the response of Penaeus vannamei to cold stress. The results indicated that cold stress disrupted nucleotide metabolism and inhibited gluconeogenesis. However, shrimp exhibited response mechanisms in order to enhance cold tolerance, regulating changes in key genes and metabolites in amino acid and lipid metabolism to increase energy supply and protect cell membrane stability. In addition, the response included regulating Ca2+ pumps and ion channels to maintain intracellular ion homeostasis and osmotic balance. When the temperature dropped further, oxidative damage occurred due to overwhelming of the antioxidant defense system, and immune function was inhibited. This research provides some references regarding the molecular mechanisms involved in responding to cold stress and potential strategies to improve cold tolerance in P. vannamei; these are important references for studying the cold stress response of shrimp.

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Section: Effects Of Cold Stress On Antioxidants Immune Defense and Os...mentioning

confidence: 99%

Effects of Cold Stress on the Hemolymph of the Pacific White Shrimp Penaeus vannamei

Zhu,

Shi,

Zhao

et al. 2024

Fishes

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“…For example, 0.1% N-acetylcysteine (NAC) effectively alleviates liver oxidation damage caused by 2.5 mg/ kg T-2 toxin, which is related to its antioxidant properties. 16 Also, 0.05% hydrated sodium calcium aluminosilicate reversed the increase of aspartate aminotransferase (AST) in broilers caused by T-2 toxin (6.0 mg/kg); 17 yeast cell wall extracts can reduce the increase in alanine aminotransferase (ALT) levels caused by T-2 toxin; 18 and selenomethionine significantly improved the liver damage of rabbits caused by T-2 toxin. 12 However, the above methods have problems, such as poor palatability and poor effect as well as being unable to alleviate the liver injury.…”

Section: ■ Introductionmentioning

confidence: 99%

Inhibition of Macrophage Pyroptosis─A New Therapeutic Strategy to Alleviate T-2 Toxin-Induced Subacute Liver Injury by Directly Competing with the Key Target

Xu,

Wu,

Zhao

et al. 2024

J. Agric. Food Chem.

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Multiple compounds are related to the development of liver injury, such as toxins, drugs, and environmental pollutants. Although there are reports that the T-2 toxin can cause liver injury, its toxic mechanism remains unclear, which further impedes the development of effective antidotes. In this study, CRISPR-Cas9 genome-wide screening technology was used to identify transformation-related protein 53 inducible nuclear protein 1 (trp53inp1) as a toxic target of the T-2 toxin. Mechanism studies have shown that the T-2 toxin induced pyroptosis of macrophages (J774A.1 cells) by activating the trp53inp1/NF-κB/NLRP3/GSDMD-N pathway, leading to a subacute liver injury. Also, the new drug berberine (BER) identified through virtual screening significantly alleviated the subacute liver injury by competitively binding trp53inp1 via His224; the effect was better than those of the positive control drugs N-acetylcysteine (NAC) and disulfiram (DSF). In summary, the above results indicate that trp53inp1 is a key target for T-2 toxin to induce subacute liver injury and that inhibiting macrophage pyroptosis is a new method for treating liver injury. In addition, this study provides a new method and strategy for the discovery of key disease targets and the search for effective drugs.

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Immune and intestinal microbiota responses to heat stress in Chinese mitten crab (Eriocheir sinensis)

Zhao

Luo

et al. 2023

Aquaculture

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N-acetylcysteine provides protection against the toxicity of dietary T-2 toxin in juvenile Chinese mitten crab (Eriocheir sinensis)

Cited by 14 publications

References 83 publications

Effects of Cold Stress on the Hemolymph of the Pacific White Shrimp Penaeus vannamei

Effects of Cold Stress on the Hemolymph of the Pacific White Shrimp Penaeus vannamei

Inhibition of Macrophage Pyroptosis─A New Therapeutic Strategy to Alleviate T-2 Toxin-Induced Subacute Liver Injury by Directly Competing with the Key Target

Immune and intestinal microbiota responses to heat stress in Chinese mitten crab (Eriocheir sinensis)

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