N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma

Nishino, Kimihiro; Yamamoto, Eiko; Niimi, Keiko; Sekiya, Yoko; Yamashita, Yoshihisa; Kikkawa, Fumitaka

doi:10.3892/or.2017.5661

Cited by 13 publications

(5 citation statements)

References 38 publications

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“…Considering GnT-IV, we knocked out both the GnT-IVa and -IVb isoenzymes 61 from HEK293 cells (Supplementary Fig. 9c ), and loss of the β1,4-GlcNAc branch from KO cells was confirmed by lectin blotting and flow cytometry using Datura stramonium agglutinin (DSA) 62 , 63 (Fig. 4k and Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan

et al. 2022

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The number of N-glycan branches on glycoproteins is closely related to the development and aggravation of various diseases. Dysregulated formation of the branch produced by N-acetylglucosaminyltransferase-V (GnT-V, also called as MGAT5) promotes cancer growth and malignancy. However, it is largely unknown how the activity of GnT-V in cells is regulated. Here, we discover that the activity of GnT-V in cells is selectively upregulated by changing cellular N-glycans from mature to immature forms. Our glycomic analysis further shows that loss of terminal modifications of N-glycans resulted in an increase in the amount of the GnT-V-produced branch. Mechanistically, shedding (cleavage and extracellular secretion) of GnT-V mediated by signal peptide peptidase-like 3 (SPPL3) protease is greatly inhibited by blocking maturation of cellular N-glycans, resulting in an increased level of GnT-V protein in cells. Alteration of cellular N-glycans hardly impairs expression or localization of SPPL3; instead, SPPL3-mediated shedding of GnT-V is shown to be regulated by N-glycans on GnT-V, suggesting that the level of GnT-V cleavage is regulated by its own N-glycan structures. These findings shed light on a mechanism of secretion-based regulation of GnT-V activity.

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Section: Resultsmentioning

confidence: 99%

Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan

et al. 2022

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“…MGAT4A is a gene that encodes for the enzyme β1-4 GlcNAc branch biosynthesis, which is known to be dysregulated in various types of cancer cells [34]. It has been reported that MGAT4A can promote cancer cell invasion by regulating glycoprotein function [35]. Additionally, the expression level of MGAT4A was found to be signi cantly increased in a mouse model of HCC induced by diethylnitrosamine injection [36].…”

Section: Discussionmentioning

confidence: 99%

Glycosylation-Related Genes predict prognosis in Hepatocellular carcinoma

Zhang,

Zhao,

Cheng

et al. 2023

Preprint

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(1) Background：Hepatocellular carcinoma (HCC) remains a significant challenge in the field of oncology despite improvements in diagnosis and treatment with surgical resection, liver transplantation, and various local therapies. Advanced HCC is particularly difficult to manage due to the lack of suitable molecular targets and drug resistance, which negatively impact prognosis. Glycosylation, an essential post-translational modification of proteins, has been implicated in HCC tumor biology, including tumor invasion, metabolism, and the immune response. This study aims to investigate the relationship between glycosylation-related genes, the immune microenvironment, and the prognosis of HCC, with the potential to identify novel prognostic biomarkers and therapeutic targets. (2) Methods and results：The TCGA database were utilized to acquire clinical data and glycosylation-related gene expression data of HCC patients, which were then used to stratify patients into high- and low-immune subgroups based on their immune score, level, and status using ESTIMATE, CIBERSORT, and ssGSEA analyses. We found that a higher immune score, level, and status were associated with a better prognosis for HCC patients. To identify potential prognostic biomarkers, we applied univariate Cox regression analysis, the LASSO algorithm, and multivariate Cox regression analysis to generate a list of eight candidate genes, which were then used to establish a prognostic risk model. Additionally, a prognostic nomogram was developed that successfully predicted the survival and prognosis of HCC patients. The identification of glycosylation-related genes and their association with the immune microenvironment in HCC offers promising clinical implications for the development of novel prognostic biomarkers and therapeutic targets. (3) Conclusions: The observed correlation between glycosylation-related genes and the immune microenvironment of hepatocellular carcinoma holds promising implications for clinical prediction. These findings suggest that glycosylation-related genes may serve as important biomarkers for predicting the immune response in hepatocellular carcinoma patients.

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“…Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) is a key enzyme in the pathway of synthesizing complex N-glycans. Several previous studies showed that MGAT4A is involved in the progression of choriocarcinoma, hepatocarcinoma, and colorectal carcinoma [ 17 – 19 ]. Chemokine receptors belong to the G-protein-coupled receptors (GPCRs) family, which appears to be involved in inflammatory diseases, tumor growth, and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Integrated Bioinformatic Analysis of Competing Endogenous RNA Network of Choriocarcinoma

Tan

Liu

et al. 2021

Med Sci Monit

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Background Numerous studies have demonstrated that noncoding RNAs are involved in choriocarcinoma (CC). The competing endogenous RNA (ceRNA) network plays an important role in the occurrence and development of carcinoma. However, the involvement of the ceRNA network in CC remains unclear. The current study aimed to investigate the regulatory mechanism of ceRNA in CC. Material/Methods We downloaded the messenger RNAs (mRNAs) expression profiles (GSE20510 and GSE65654) and microRNAs (miRNAs) expression profiles (GSE32346 and GSE130489) from GEO datasets. The limma package of R software was used to identify differentially expressed RNAs (DERNAs). Then, we performed functional annotation of the differentially expressed mRNAs (DEmRNAs). TargetScan, miRDB, miRWalk, and Starbase were used to construct a CC-specific ceRNA network and select key molecules. Results The results identified a total of 177 DEmRNAs and 189 differentially expressed miRNAs (DEmiRNAs) between the trophoblast and CC cell line samples. Ten differentially expressed lncRNAs (DElncRNAs) were obtained based on experimental studies. The DEmRNAs were mainly enriched in cell proliferation, positive regulation of the apoptotic process, and cell death. A total of 10 genes were ascertained as hub genes. Based on DEmRNAs, DEmiRNAs, and DElncRNAs, a CC-specific ceRNA network was established. Five DElncRNAs, 15 DEmiRNAs, and 45 DEmRNAs were identified. In addition, LINC00261, MEG3, MALAT1, H19, and OGFRP1 were identified as 5 key lncRNAs in choriocarcinoma. Conclusions This study provides novel insights into CC mechanisms and identified potential therapeutic targets for CC.

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N-acetylglucosaminyltransferase IVa promotes invasion of choriocarcinoma

Cited by 13 publications

References 38 publications

Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan

Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan

Glycosylation-Related Genes predict prognosis in Hepatocellular carcinoma

Integrated Bioinformatic Analysis of Competing Endogenous RNA Network of Choriocarcinoma

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