N-acyl Homoserine Lactone-Producing Pseudomonas putida Strain T2-2 from Human Tongue Surface

Chen, Jian Woon; Chin, Shenyang; Tee, Kok Keng; Yin, Wai Fong; Choo, Yeun‐Mun; Chan, Kok‐Gan

doi:10.3390/s131013192

Cited by 15 publications

(22 citation statements)

References 59 publications

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“…From RAST annotation, the closest neighbours of T2-2 were P. putida B6-2 (score 545), followed by P. putida F1 (score 502) and P. putida KT2440 and P. putida GB-1 (score 494). The present whole genome analysis of bacterial identification was in agreement with the previous report that relied on phylogenetics analysis of its 16S rDNA (Accession number: HQ907954) which clearly identified T2-2 as P. putida [ 13 ]. The 5,053 CDSs predicted by RAST were divided into subsystems and shown in Figure 1 .…”

Section: Resultssupporting

confidence: 91%

“…Isolation and characterization of bacteria strain T2-2 have been described by Chen et al, 2013 [ 13 ]. Strain T2-2 was maintained and cultured in LB medium.…”

Section: Methodsmentioning

confidence: 99%

“…As a species commonly found in the environment, association of P. putida as commensal organism in human is rarely described. A strain of the species, P. putida T2-2, had been isolated from a healthy individual's tongue surface and reported for its quorum sensing activity [ 13 ]. As there is reason to believe that the species is an opportunistic pathogen with ability to cause life-threatening diseases in human, it raises our concern about the emergence of the multidrug resistant strains.…”

Section: Introductionmentioning

confidence: 99%

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Global Genome Comparative Analysis Reveals Insights of Resistome and Life-Style Adaptation ofPseudomonas putidaStrain T2-2 in Oral Cavity

Chan

Chua

How

et al. 2014

The Scientific World Journal

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Most Pseudomonas putida strains are environmental microorganisms exhibiting a wide range of metabolic capability but certain strains have been reported as rare opportunistic pathogens and some emerged as multidrug resistant P. putida. This study aimed to assess the drug resistance profile of, via whole genome analysis, P. putida strain T2-2 isolated from oral cavity. At the same time, we also compared the nonenvironmental strain with environmentally isolated P. putida. In silico comparative genome analysis with available reference strains of P. putida shows that T2-2 has lesser gene counts on carbohydrate and aromatic compounds metabolisms, which suggested its little versatility. The detection of its edd gene also suggested T2-2's catabolism of glucose via ED pathway instead of EMP pathway. On the other hand, its drug resistance profile was observed via in silico gene prediction and most of the genes found were in agreement with drug-susceptibility testing in laboratory by automated VITEK 2. In addition, the finding of putative genes of multidrug resistance efflux pump and ATP-binding cassette transporters in this strain suggests a multidrug resistant phenotype. In summary, it is believed that multiple metabolic characteristics and drug resistance in P. putida strain T2-2 helped in its survival in human oral cavity.

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Section: Resultssupporting

confidence: 91%

“…Isolation and characterization of bacteria strain T2-2 have been described by Chen et al, 2013 [ 13 ]. Strain T2-2 was maintained and cultured in LB medium.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Global Genome Comparative Analysis Reveals Insights of Resistome and Life-Style Adaptation ofPseudomonas putidaStrain T2-2 in Oral Cavity

Chan

Chua

How

et al. 2014

The Scientific World Journal

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“…The effect of bacteria on fungal growth was assessed using an agar plate method described by Kerr (1999) , with slight modifications adopted from Chen et al (2013) . P. aeruginosa strains PASS1, PASS2, and PAO1; and S. aurantiacum strains WM 06.482 and WM 08.202, were cultured together on a synthetic cystic fibrosis medium (SCFM) that mimics the nutritional content of human CF sputum.…”

Section: Methodsmentioning

confidence: 99%

Pseudomonas aeruginosa inhibits the growth of Scedosporium aurantiacum, an opportunistic fungal pathogen isolated from the lungs of cystic fibrosis patients

et al. 2015

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The filamentous fungus Scedosporium aurantiacum and the bacterium Pseudomonas aeruginosa are opportunistic pathogens isolated from lungs of the cystic fibrosis (CF) patients. P. aeruginosa has been known to suppress the growth of a number of CF related fungi such as Aspergillus fumigatus, Candida albicans, and Cryptococcus neoformans. However, the interactions between P. aeruginosa and S. aurantiacum have not been investigated in depth. Hence we assessed the effect of P. aeruginosa reference strain PAO1 and two clinical isolates PASS1 and PASS2 on the growth of two clinical S. aurantiacum isolates WM 06.482 and WM 08.202 using solid plate assays and liquid cultures, in a synthetic medium mimicking the nutrient condition in the CF sputum. Solid plate assays showed a clear inhibition of growth of both S. aurantiacum strains when cultured with P. aeruginosa strains PASS1 and PAO1. The inhibitory effect was confirmed by confocal microscopy. In addition to using chemical fluorescent stains, strains tagged with yfp (P. aeruginosa PASS1) and mCherry (S. aurantiacum WM 06.482) were created to facilitate detailed microscopic observations on strain interaction. To our knowledge, this is the first study describing successful genetic transformation of S. aurantiacum. Inhibition of growth was observed only in co-cultures of P. aeruginosa and S. aurantiacum; the cell fractions obtained from independent bacterial monocultures failed to initiate a response against the fungus. In the liquid co-cultures, biofilm forming P. aeruginosa strains PASS1 and PAO1 displayed higher inhibition of fungal growth when compared to PASS2. No change was observed in the inhibition pattern when direct cell contact between the bacterial and fungal strains was prevented using a separation membrane suggesting the involvement of extracellular metabolites in the fungal inhibition. However, one of the most commonly described bacterial virulence factors, pyocyanin, had no effect against either of the S. aurantiacum strains. This study shows that P. aeruginosa has a substantial inhibitory effect on the growth of the recently described CF fungal pathogen S. aurantiacum. The findings also highlighted that P. aeruginosa biofilm formation is important but not crucial for inhibiting the growth of S. aurantiacum in a lung- mimicking environment.

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“…There has been little evidence for AHL-mediated signalling in oral microbial communities, although a Pseudomonas species producing AHL has recently been isolated from the tongue surface [17]. AHLs have been shown to be chemoattractants for neutrophils, alter barrier integrity of epithelial cells, and increase macrophage phagocytic activity [18].…”

Section: Bacterial Wires and Mobilesmentioning

confidence: 99%

Interkingdom networking within the oral microbiome

Nobbs

Jenkinson

2015

Microbes and Infection

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N-acyl Homoserine Lactone-Producing Pseudomonas putida Strain T2-2 from Human Tongue Surface

Cited by 15 publications

References 59 publications

Global Genome Comparative Analysis Reveals Insights of Resistome and Life-Style Adaptation ofPseudomonas putidaStrain T2-2 in Oral Cavity

Global Genome Comparative Analysis Reveals Insights of Resistome and Life-Style Adaptation ofPseudomonas putidaStrain T2-2 in Oral Cavity

Pseudomonas aeruginosa inhibits the growth of Scedosporium aurantiacum, an opportunistic fungal pathogen isolated from the lungs of cystic fibrosis patients

Interkingdom networking within the oral microbiome

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