N-acyl-homoserine-lactone quorum sensing in tomato phytopathogenic Pseudomonas spp. is involved in the regulation of lipodepsipeptide production

Licciardello, Grazia; Strano, C. P.; Bertani, Iris; Bella, P.; Fiore, Alberto; Fogliano, Vincenzo; Venturi, Vittorio; Catara, Vittoria

doi:10.1016/j.jbiotec.2011.07.036

Cited by 29 publications

(44 citation statements)

References 45 publications

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“…Pseudomonas corrugata mutants in the genes coding for the transcriptional regulators pcoR (GL2 mutant) and rfiA (GLRFIA mutant) no longer produced CLPs (Licciardello et al ., ). By investigating the expression of crpC and crpDE in both the GL2 and GLRFIA mutant strains, we observed that transcript levels were significantly and consistently reduced in both mutant strains (data not shown).…”

Section: Resultsmentioning

confidence: 97%

“…To investigate the role of crpCDE in P. corrugata –plant interaction, we set up in planta assays. The tests were performed with the PCOMFP and PCONRPS mutant strains (producing only cormycin) and, as controls, the parent strain CFBP 5454 (which produces both cormycin and corpeptins) and the GLRFIA mutant strain ( rfiA – ) (which is no longer able to produce both cormycin and corpeptins) (Licciardello et al ., ).…”

Section: Resultsmentioning

confidence: 97%

“…Recently, we have demonstrated that the AHL-QS system of P. corrugate, PcoI/PcoR, and the transcriptional regulator RfiA play an important role in the regulation of virulence via CLP production (Licciardello et al, 2012). The absence of further genetic and molecular information prompted us to sequence the complete cosmid insert from which the AHL-QS genes were identified ( Fig.…”

Section: Analysis Of a Putative Abc Efflux System And A Putative Nrpsmentioning

confidence: 99%

“…Pseudomonas corrugata pcoR and rfiA mutants show significantly reduced virulence when inoculated in tomatoes. CLPs were also absent in their culture filtrates (Licciardello et al, 2009(Licciardello et al, , 2012. Genetically linked to rfiA is the operon pcoABC, which is under positive regulation by RfiA, and thus indirectly by the PcoI/R system (Licciardello et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Pseudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in Nicotiana benthamiana

Strano

Bella

Licciardello

et al. 2014

Molecular Plant Pathology

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Pseudomonas corrugata CFBP 5454 produces two kinds of cyclic lipopeptides (CLPs), cormycin A and corpeptins, both of which possess surfactant, antimicrobial and phytotoxic activities. In this study, we identified genes coding for a putative non-ribosomal peptide synthetase and an ABC-type transport system involved in corpeptin production. These genes belong to the same transcriptional unit, designated crpCDE. The genetic organization of this locus is highly similar to other Pseudomonas CLP biosynthetic clusters. Matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that transporter and synthetase genomic knock-out mutants were unable to produce corpeptins, but continued to produce cormycin A. This suggests that CrpCDE is the only system involved in corpeptin production in P. corrugata CFBP 5454. In addition, phylogenetic analysis revealed that the CrpE ABC transporter clustered with the transporters of CLPs with a long peptide chain. Strains depleted in corpeptin production were significantly less virulent than the wild-type strain when inoculated in tomato plants and induced only chlorosis when infiltrated into Nicotiana benthamiana leaves. Thus, corpeptins are important effectors of P. corrugata interaction with plants. Expression analysis revealed that crpC transcription occurs at high cell density. Two LuxR transcriptional regulators, PcoR and RfiA, have a pivotal role in crpC expression and thus in corpeptin production.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Analysis Of a Putative Abc Efflux System And A Putative Nrpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pseudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in Nicotiana benthamiana

Strano

Bella

Licciardello

et al. 2014

Molecular Plant Pathology

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“…AHLs regulate in a cell-density-dependent manner a variety of physiological processes, such as bioluminescence [15], swarming and swimming motility [16, 17], biofilm differentiation and development [18–22], secondary metabolite production [23–26], conjugation and virulence gene expression (for reviews see Ref. [27–31]).…”

Section: Introductionmentioning

confidence: 99%

The Systematic Investigation of the Quorum Sensing System of the Biocontrol Strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 Unveils aurI to Be a Biosynthetic Origin for 3-Oxo-Homoserine Lactones

et al. 2016

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The shoot endophytic biocontrol strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 produces a wide range of exoproducts, including enzymes and antibiotics. The production of exoproducts is commonly tightly regulated. In order to get a deeper insight into the regulatory network of PB-St2, the strain was systematically investigated regarding its quorum sensing systems, both on the genetic and metabolic level. The genome analysis of PB-St2 revealed the presence of four putative acyl homoserine lactone (AHL) biosynthesis genes: phzI, csaI, aurI, and hdtS. LC-MS/MS analyses of the crude supernatant extracts demonstrated that PB-St2 produces eight AHLs. In addition, the concentration of all AHL derivatives was quantified time-resolved in parallel over a period of 42 h during the growth of P. aurantiaca PB-St2, resulting in production curves, which showed differences regarding the maximum levels of the AHLs (14.6 nM– 1.75 μM) and the production period. Cloning and heterologous overexpression of all identified AHL synthase genes in Escherichia coli proved the functionality of the resulting synthases PhzI, CsaI, and AurI. A clear AHL production pattern was assigned to each of these three AHL synthases, while the HdtS synthase did not lead to any AHL production. Furthermore, the heterologous expression study demonstrated unequivocally and for the first time that AurI directs the synthesis of two 3-oxo-AHLs.

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Biosynthesis of the antimicrobial cyclic lipopeptides nunamycin and nunapeptin by Pseudomonas fluorescens strain In5 is regulated by the LuxR‐type transcriptional regulator NunF

et al. 2017

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Nunamycin and nunapeptin are two antimicrobial cyclic lipopeptides (CLPs) produced by Pseudomonas fluorescens In5 and synthesized by nonribosomal synthetases (NRPS) located on two gene clusters designated the nun–nup regulon. Organization of the regulon is similar to clusters found in other CLP‐producing pseudomonads except for the border regions where putative LuxR‐type regulators are located. This study focuses on understanding the regulatory role of the LuxR‐type‐encoding gene nunF in CLP production of P. fluorescens In5. Functional analysis of nunF coupled with liquid chromatography–high‐resolution mass spectrometry (LC‐HRMS) showed that CLP biosynthesis is regulated by nunF. Quantitative real‐time PCR analysis indicated that transcription of the NRPS genes catalyzing CLP production is strongly reduced when nunF is mutated indicating that nunF is part of the nun–nup regulon. Swarming and biofilm formation was reduced in a nunF knockout mutant suggesting that these CLPs may also play a role in these phenomena as observed in other pseudomonads. Fusion of the nunF promoter region to mCherry showed that nunF is strongly upregulated in response to carbon sources indicating the presence of a fungus suggesting that environmental elicitors may also influence nunF expression which upon activation regulates nunamycin and nunapeptin production required for the growth inhibition of phytopathogens.

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N-acyl-homoserine-lactone quorum sensing in tomato phytopathogenic Pseudomonas spp. is involved in the regulation of lipodepsipeptide production

Cited by 29 publications

References 45 publications

Pseudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in Nicotiana benthamiana

Pseudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in Nicotiana benthamiana

The Systematic Investigation of the Quorum Sensing System of the Biocontrol Strain Pseudomonas chlororaphis subsp. aurantiaca PB-St2 Unveils aurI to Be a Biosynthetic Origin for 3-Oxo-Homoserine Lactones

Biosynthesis of the antimicrobial cyclic lipopeptides nunamycin and nunapeptin by Pseudomonas fluorescens strain In5 is regulated by the LuxR‐type transcriptional regulator NunF

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