N -Acyl-l-Homoserine Lactone Autoinducers Control Production of an Extracellular Lipopeptide Biosurfactant Required for Swarming Motility of Serratia liquefaciens MG1

Lindum, Peter W.; Anthoni, U.; Christophersen, Camilla; Eberl, Leo; Molin, Sebastian; Givskov, Michael

doi:10.1128/.180.23.6384-6388.1998

Cited by 31 publications

(29 citation statements)

References 24 publications

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“…As seen in Fig. 3, media supplemented with purified serrawettin W2, allows the swrI mutant to travel across the agar surface [72]. One has to realize that inactivation of swrI neither affects growth rate or swimming motility, nor the development of hyperflagellation and cell elongation [73,74].…”

Section: Serrawettin Production By the Genus Serratiamentioning

confidence: 99%

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Quorum sensing and swarming migration in bacteria

2004

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Bacterial cells can produce and sense signal molecules, allowing the whole population to initiate a concerted action once a critical concentration (corresponding to a particular population density) of the signal has been reached, a phenomenon known as quorum sensing. One of the possible quorum sensing-regulated phenotypes is swarming, a flagella-driven movement of differentiated swarmer cells (hyperflagellated, elongated, multinucleated) by which bacteria can spread as a biofilm over a surface. The glycolipid or lipopeptide biosurfactants thereby produced function as wetting agent by reducing the surface tension. Quorum sensing systems are almost always integrated into other regulatory circuits. This effectively expands the range of environmental signals that influence target gene expression beyond population density. In this review, we first discuss the regulation of AHL-mediated surface migration and the involvement of other low-molecular-mass signal molecules (such as the furanosyl borate diester AI-2) in biosurfactant production of different bacteria. In addition, population density-dependent regulation of swarmer cell differentiation is reviewed. Also, several examples of interspecies signalling are reported. Different signal molecules either produced by bacteria (such as other AHLs and diketopiperazines) or excreted by plants (such as furanones, plant signal mimics) might influence the quorum sensing-regulated swarming behaviour in bacteria different from the producer. On the other hand, specific bacteria can reduce the local available concentration of signal molecules produced by others. In the last part, the role and regulation of a surface-associated movement in biofilm formation is discussed. Here we also describe how quorum sensing may disperse existing biofilms and control the interaction between bacteria and higher organisms (such as the Rhizobium-bean symbiosis).

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Section: Serrawettin Production By the Genus Serratiamentioning

confidence: 99%

“…(B) Effect of serrawettin W2 on the surface tension of water. The droplets contain 0, 0.5, 0.8, 1, 2 and 40 lg/ml serrawettin (taken from[72]).…”

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confidence: 99%

Quorum sensing and swarming migration in bacteria

2004

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“…Understanding this has already led to the use of competitive antagonists (such as furanones) that can interfere with the signals that trigger bio¢lm di¡erentiation and decrease P. aeruginosa bio¢lm formation, for example [464 8]. For some bacteria, for example Serratia liquefaciens (which has one £agellar type for both swimming and swarming), QS has been shown to also in£uence the production of surfactant required for swarming motility on surfaces [49].…”

Section: Flagellar Motilities : Their Roles In Colonization Of Surfacmentioning

confidence: 99%

Bacteria that express lateral flagella enable dissection of the multifunctional roles of flagella in pathogenesis

Kirov¹

2003

FEMS Microbiology Letters

101

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Flagella are much more than organelles of locomotion and have multiple roles that contribute to pathogenesis. Bacteria, such as Vibrio parahaemolyticus and Aeromonas spp., that possess two distinct flagellar systems (a polar flagellum for swimming in liquid and lateral flagella for swarming over surfaces) are relatively uncommon and provide ideal models for the independent investigation of the contributions of these different types of motility and other flagellar functions to virulence and how they are controlled. Studies with the above organisms have already increased our understanding of how bacteria sense and colonize surfaces forming biofilms that enable them to survive and persist in hostile environments. These insights are helping to identify possible new targets for novel antimicrobials that will both prevent or disrupt these processes and enhance the effectiveness of existing antibiotics. Aeromonas lateral flagella, in addition to mediating swarming motility, appear to be adhesins in their own right, contribute to microcolony formation and efficient biofilm formation on surfaces, and possibly facilitate host cell invasion. It is, therefore, likely that the ability to express lateral flagella is a significant virulence determinant for the Aeromonas strains able to cause persistent and dysenteric infections in the gastrointestinal tract, but further work is needed to establish this.

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“…data). Quorum sensing controls attachment, swarming, exoenzyme secretion and biofilm formation in S. marcescens (Eberl et al ., 1996;Lindum et al ., 1998;Riedel et al ., 2001;Labbate et al ., 2004). This demonstrates that QS is broadly involved in surface-associated phenotypes and the secretion of extracellular products in S. marcescens .…”

Section: Introductionmentioning

confidence: 99%

The role of quorum sensing mediated developmental traits in the resistance of Serratia marcescens biofilms against protozoan grazing

Queck

Weitere

Moreno

et al. 2006

Environmental Microbiology

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Resistance against protozoan grazers is a crucial factor that is important for the survival of many bacteria in their natural environment. However, the basis of resistance to protozoans and how resistance factors are regulated is poorly understood. In part, resistance may be due to biofilm formation, which is known to protect bacteria from environmental stress conditions. The ubiquitous organism Serratia marcescens uses quorum sensing (QS) control to regulate virulence factor expression and biofilm formation. We hypothesized that the QS system of S. marcescens also regulates mechanisms that protect biofilms against protozoan grazing. To investigate this hypothesis, we compared the interactions of wild-type and QS mutant strains of S. marcescens biofilms with two protozoans having different feeding types under batch and flow conditions. Under batch conditions, S. marcescens forms microcolony biofilms, and filamentous biofilms are formed under flow conditions. The microcolony-type biofilms were protected from grazing by the suspension feeder, flagellate Bodo saltans, but were not protected from the surface feeder, Acanthamoeba polyphaga. In contrast, the filamentous biofilm provided protection against A. polyphaga. The main findings presented in this study suggest that (i) the QS system is not involved in grazing resistance of S. marcescens microcolony-type biofilms; (ii) QS in S. marcescens regulates antiprotozoan factor(s) that do not interfere with the grazing efficiency of the protozoans; and (iii) QS-controlled, biofilm-specific differentiation of filaments and cell chains in biofilms of S. marcescens provides an efficient mechanism against protozoan grazing.

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N -Acyl-l-Homoserine Lactone Autoinducers Control Production of an Extracellular Lipopeptide Biosurfactant Required for Swarming Motility of Serratia liquefaciens MG1

Cited by 31 publications

References 24 publications

Quorum sensing and swarming migration in bacteria

Quorum sensing and swarming migration in bacteria

Bacteria that express lateral flagella enable dissection of the multifunctional roles of flagella in pathogenesis

The role of quorum sensing mediated developmental traits in the resistance of Serratia marcescens biofilms against protozoan grazing

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