n-Butyrate inhibition of hyaluronate synthesis in cultured human fibroblasts.

Abstract

The effects of the short-chain aliphatic carboxylic acid, n-butyrate, on glycosaminoglycan (GAG) accumulation were studied in cultured human skin fibroblasts. Normal fibroblast cultures were grown to confluence, shifted to a medium without or with n-butyrate for 24 h, labeled with either VHlacetate or V3Hjglucosamine and analyzed for IHIGAG and V3Hjhyaluronate accumulation. Accumulation was stimulated at low concentrations (0.1-1 mM) by up to 27%. Higher concentrations of n-butyrate (> 1 mM) inhibited V3HIGAG by up to 70-90%. This effect was maximal at 10 mM and half-maximal at 3 mM. Propionate had similar effects but was less potent. Parallel studies conducted in colonic fibroblasts revealed that n-butyrate could markedly inhibit I3HIGAG accumulation in that cell type as well. These effects were rapid, occurring within 3 h of treatment, and were reversible. Chondroitin sulfate accumulation was unaffected by the compound. A pulse-chase study failed to demonstrate any effect on 13HIGAG degradation.

Up-regulation of Prostaglandin E2 Synthesis by Interleukin-1β in Human Orbital Fibroblasts Involves Coordinate Induction of Prostaglandin-Endoperoxide H Synthase-2 and Glutathione-dependent Prostaglandin E2 Synthase Expression

“…Tissue fragments were generated by mechanical disruption of explants, and fibroblasts were then allowed to adhere to plastic culture plates. They were covered with Eagle's medium to which 10% fetal bovine serum (FBS), glutamine (435 g/ml), and penicillin/ streptomycin were added as described previously (33). Medium was changed every 3-4 days, and monolayers were maintained in a 5% CO 2 , humidified incubator at 37°C.…”

IL-1β Induces IL-6 Expression in Human Orbital Fibroblasts: Identification of an Anatomic-Site Specific Phenotypic Attribute Relevant to Thyroid-Associated Ophthalmopathy

“…Some of the fibroblast strains were kindly provided by Dr. R. Bahn (Mayo Clinic, Rochester, MN). Tissue fragments were generated by mechanical disruption of surgical explants, and fibroblasts were then allowed to outgrow the tissue and adhere to plastic culture plates (29). They were covered with Eagle's medium to which 10% FBS, glutamine (435 g/ml), and penicillin were added as described previously (29).…”

“…Tissue fragments were generated by mechanical disruption of surgical explants, and fibroblasts were then allowed to outgrow the tissue and adhere to plastic culture plates (29). They were covered with Eagle's medium to which 10% FBS, glutamine (435 g/ml), and penicillin were added as described previously (29). Several dermal fibroblast strains were either generated from punch biopsies of normal appearing skin or were purchased from the American Type Culture Collection.…”

Cultured Human Fibroblasts Express Constitutive IL-16 mRNA: Cytokine Induction of Active IL-16 Protein Synthesis Through a Caspase-3-Dependent Mechanism

“…Substantial evidence supports the concept that fibroblasts from different anatomic regions exhibit distinct phenotypes in culture. Characteristic expression patterns of receptors (15), gangliosides (16,17), glycosaminoglycans (17)(18)(19)(20)(21)(22), plasminogen activator inhibitor type 1 (23)(24)(25)(26), and PG endoperoxide H synthase-2 (27) have recently been documented in different populations of fibroblasts. Moreover, fibroblasts are capable of responding to their microenvironment in a complex manner.…”