N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells

Greenbaum, Adam; Revollo, Leila; Woloszynek, Jill; Civitelli, Roberto; Link, Daniel C.

doi:10.1182/blood-2011-09-377457

Cited by 83 publications

(72 citation statements)

References 38 publications

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“…In the HSC niche, HSCs and osteoblasts express N-cadherin (Zhang et al, 2003), and multiple studies have suggested that N-cadherin is required for maintaining HSCs in the niche for long-term self-renewal (Haug et al, 2008;Hosokawa et al, 2010a;Hosokawa et al, 2010b). However, other independent studies have disputed the role of Ncadherin in controlling HSC self-renewal (Bromberg et al, 2012;Greenbaum et al, 2012;Kiel et al, 2009). The contradictory conclusions concerning the role of N-cadherin in the regulation of HSC self-renewal could arise from different experimental methods.…”

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confidence: 99%

Adhesion in the stem cell niche: biological roles and regulation

2013

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Stem cell self-renewal is tightly controlled by the concerted action of stem cell-intrinsic factors and signals within the niche. Niche signals often function within a short range, allowing cells in the niche to self-renew while their daughters outside the niche differentiate. Thus, in order for stem cells to continuously self-renew, they are often anchored in the niche via adhesion molecules. In addition to niche anchoring, however, recent studies have revealed other important roles for adhesion molecules in the regulation of stem cell function, and it is clear that stem cell-niche adhesion is crucial for stem cell self-renewal and is dynamically regulated. Here, we highlight recent progress in understanding adhesion between stem cells and their niche and how this adhesion is regulated.

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confidence: 99%

Adhesion in the stem cell niche: biological roles and regulation

2013

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“…The osteoblasts are assumed to contact the HSCs by the way of direct interaction via N-cadherin-mediated adhesion [63]. However, with the use of conditional knockout of N-cadherin (Cdh2) in the hematopoietic cells [112], osteoprogenitors [113] and osteoblasts [114] no essential changes in the number of HSCs was seen although the N-cadherin overexpression somewhat increased their number [115,116].…”

Section: Cd45mentioning

confidence: 99%

Structural-functional organisation of the bone marrow hematopoietic stem cells niches

Nikolskaya¹,

Butenko²

2016

JCOT

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This article focuses on (1) the analysis of the structural-functional organization of bone marrow niches of the hematopoietic stem cells, (2) the role of the intercellular contact interactions and humoral regulation factors in these niches, in particular CXCL12, SCF and TGFβ,and (3) KEYWORDS: bone marrow, hematopoietic stem cell niche, multipotent stromal cells, hematopoiesisMaksimov and finally established at the end of the past century, it is not only the number but also entire known diversity of cell elements of immune and blood systems originate from only one type of the progenitor elements -the HSCs (unitary theory) [2].Life force and productivity of the HSCs is proved, for example, by the fact that 99.9 % of all blood-generating cells, HSCs included, in the irradiated mice can perish, and the surviving stem cells rapidly renew blood formation, including proper population as well as all types of the committed progenitors [3]. Along same sequence, the fact of non-exhaustion of blood formation after repeated damaging exposures is explained by the presence and functioning of the HSCs [1].The specialized cells of adult and fetal periods of ontogenesis, residing in the bone marrow (BM) in the quiescent state and capable, together with blood-forming microenvironment, to realize underlying programs for self-maintenance and multipotency allowing them (with maintained amount of HSCs) to release necessary amount of cells into differentiation along various directions that ultimately leads to the formation of all forms of blood elements, including immune system cells. It should be noted that the self-maintenance is not identical to the immortality. Stem cells are characterized by sufficiently long life, commensurable with entire organism's life course. Several genetic regulatory programs have been established which are of great importance in the process of HSCs self-maintenance. It is also known that stem cells in various tissues are controlled by common genetic programs maintaining their nature [4,5].It is theorized that stem cells are the clonogenic units which under certain conditions can be increased in their number at the expense Immune system is the structural-functional and interrelated commonness of the hematopoietic and stromal cells. Different in their origin, structure and functions, the populations and subpopulations of lymphoid and myeloid cells make a complete wholeness in the definite tissues and organs where they closely cooperate with non-hematopoietic elements. Constant and strictly regulated intensity of hematopoiesis with production of various cell types is also conditioned and controlled by cooperation of the hematopoietic and stromal cells. Timely repopulation of the immune system with hematopoietic cells-precursors, lymphocytes, leucocytes and stromal cells, newly maturing in the bone marrow, is the main condition for effective immune system functioning. The number of cells formed per time unit is plentiful. According to the given data, nearly 10 11 neutrophils (about 100 g of mass) per...

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“…These cells also express high levels of SCF and CXCL12 and support long term repopulating activity of HSCs [24]. In this respect, N-cadherin knockdown or expression of a dominant negative molecule in HSCs inhibited their repopulating capacity [25,26] but conditional deletion of N-cadherin encoding gene in HSC had no effects on HSC number, mobilization, proliferation or repopulating capacity [25,27].…”

Section: The Existence Of An Endosteal Niche In the Adult Bm Is Contrmentioning

confidence: 99%

The Adult Hematopoietic Niches — Cellular Composition, Histological Organization and Physiological Regulation

Zapata¹

2014

Adult Stem Cell Niches

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N-cadherin in osteolineage cells is not required for maintenance of hematopoietic stem cells

Cited by 83 publications

References 38 publications

Adhesion in the stem cell niche: biological roles and regulation

Adhesion in the stem cell niche: biological roles and regulation

Structural-functional organisation of the bone marrow hematopoietic stem cells niches

The Adult Hematopoietic Niches — Cellular Composition, Histological Organization and Physiological Regulation

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