N-cadherin in osteolineage cells modulates stromal support of tumor growth

Fontana, Francesca; Xiang, Jingyu; Su, Xinming; Tycksen, Eric; Nassau, Rachel; Fox, Gregory C.; Leanza, Giulia; Weilbaecher, Katherine N.; Civitelli, Roberto

doi:10.1016/j.jbo.2021.100356

Cited by 1 publication

(1 citation statement)

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“…In another study, anti-EGFR monoclonal antibody decreased the movement of H322 and A549 cells due to increased expression of E-cadherin after treatment with mAb. These results also suggested that E-cadherin could act as a biomarker for benign NSCLC cancers 26,27 . Overall, the study implies that E-cadherin could be a biomarker for diagnosis of Non-small Cell Lung Cancer (NSCLC) cancer and our results demonstrate the possible use of Genistein as a chemotherapeutic agent for the treatment of cancer.…”

Section: Discussionmentioning

confidence: 62%

Effect of Genistein on E-Cadherin as Biomarkers in Diagnosis and Treatment of Lung Cancer

Li¹,

Yu²,

Chen³

et al. 2021

International J. of Pharmacology

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Background and Objective: The prevalence of lung cancer is significant enough in recent times and the research is not able to provide availability of treatment for a late-stage cancer patient to increase the survival time and rate. Among many established markers, E-cadherin (involved in cell-cell interactions) has a crucial role to play in subsiding tumour growth and its malignancy. Therefore, the study aims to investigate the effects of Genistein on cell viability on lung cancer cell lines and its expression patterns on E-cadherin and EGFR. Materials and Methods: Lung cancer cell lines were grown and assayed for cell viability for Genistein compared with Cisplatin. The protein samples were further extracted with radio-immunoprecipitation assay and carried forward for western and northern blotting. Furthermore, the effect of Genistein as apoptotic cell death-inducing activity was analyzed using a flow cytometer and estimated with Caspase 3 expression. Results: In the presence of Genistein, cell lines A549, NCI-H820, NCI-H838, NCI-H1155, HCC4006 and H322 showed cell viability less than 40%, at varying concentrations. Genistein at concentration 0.1 µM showed cell viability less than 20% in cell lines NCI-H820, NCI-H838, NCI-H1155, HCC4006 and H322. Further western blot showed the expression of E-cadherin in all cell lines signifying them as biomarkers in NSCLC treatment. Results from flow cytometry also supported the significant effects of Genistein on NSCLC cell lines. Conclusion:The study proposes E-cadherin could be a biomarker for diagnosis of Non-small Cell Lung Cancer (NSCLC) and Genistein remediated up-regulation of E-cadherin could prove to be a novel outcome for the treatment of cancer and prevent malignancy.

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Section: Discussionmentioning

confidence: 62%