N-Cadherin is Involved in Neuronal Activity-Dependent Regulation of Myelinating Capacity of Zebrafish Individual Oligodendrocytes In Vivo

Chen, Min; Xu, Yang; Huang, Rongchen; Huang, Yubin; Ge, Shuchao; Hu, Bing

doi:10.1007/s12035-016-0233-4

Cited by 22 publications

(15 citation statements)

References 69 publications

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“…Next to the extracellular matrix proteins such as laminin‐2/merosin, adhesion molecules have been proposed to function as permissive signals for the initiation of myelination; these include the neural cell adhesion molecules L1 (Laursen, Chan, & ffrench‐Constant, ), N‐cadherin (Chen et al, ; Schnadelbach, Ozen, Blaschuk, Meyer, & Fawcett, ), and Necl‐1 (Park et al, ). The implicated diversity of permissive cues suggests that extrinsically modulated myelination of axons may be regulated by local molecular signatures that identify individual subpopulations of axons to be myelinated.…”

Section: The Growth Cone and Its Actin Cytoskeleton As A Driver Of Dymentioning

confidence: 99%

The oligodendrocyte growth cone and its actin cytoskeleton: A fundamental element for progenitor cell migration and CNS myelination

Thomason

Escalante

Osterhout

et al. 2019

Glia

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Cells of the oligodendrocyte (OLG) lineage engage in highly motile behaviors that are crucial for effective central nervous system (CNS) myelination. These behaviors include the guided migration of OLG progenitor cells (OPCs), the surveying of local environments by cellular processes extending from differentiating and premyelinating OLGs, and during the process of active myelin wrapping, the forward movement of the leading edge of the myelin sheath's inner tongue along the axon.Almost all of these motile behaviors are driven by actin cytoskeletal dynamics initiated within a lamellipodial structure that is located at the tip of cellular OLG/OPC processes and is structurally as well as functionally similar to the neuronal growth cone. Accordingly, coordinated stoichiometries of actin filament (F-actin) assembly and disassembly at these OLG/OPC growth cones have been implicated in directing process outgrowth and guidance, and the initiation of myelination. Nonetheless, the functional importance of the OLG/OPC growth cone still remains to be fully understood, and, as a unique aspect of actin cytoskeletal dynamics, F-actin depolymerization and disassembly start to predominate at the transition from myelination initiation to myelin wrapping. This review provides an overview of the current knowledge about OLG/OPC growth cones, and it proposes a model in which actin cytoskeletal dynamics in OLG/OPC growth cones are a main driver for morphological transformations and motile behaviors. Remarkably, these activities, at least at the later stages of OLG maturation, may be regulated independently from the transcriptional gene expression changes typically associated with CNS myelination. K E Y W O R D Sactin cytoskeleton, growth cone, migration, myelination, oligodendrocyte

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Section: The Growth Cone and Its Actin Cytoskeleton As A Driver Of Dymentioning

confidence: 99%

The oligodendrocyte growth cone and its actin cytoskeleton: A fundamental element for progenitor cell migration and CNS myelination

Thomason

Escalante

Osterhout

et al. 2019

Glia

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“…Mitochondria are the main source of cellular energy, whereas axonal regeneration is a process costing huge amounts of energy. Previously, we reported that axonal regeneration was correlated with mitochondrial motility (44). Furthermore, we found that mitochondrial movement was correlated with the level of neuronal activity.…”

Section: Discussionmentioning

confidence: 55%

“…Single-cell electroporation was performed at 4 dpf as previously described (44,45). We transferred the anesthetized larvae onto the homemade electroporation chamber using a plastic transfer pipette and carefully positioned the larvae dorsal side up in 1% low-melting agarose.…”

Section: Single-cell Electroporationmentioning

confidence: 99%

“…To further test our hypothesis that the evoked calcium response indicated intrinsic regenerative ability and study the effect of neural activity on axonal regeneration, we overexpressed Kir2.1a, an inward rectifying potassium channel protein that down-regulates neuronal activity (44,(50)(51)(52), in zebrafish M cells using single-cell electroporation at 4 dpf. We expected that the overexpression of Kir2.1a would influence calcium dynamics by down-regulating neuronal activity.…”

Section: Kir21a Inhibited Neural Activity and Axonal Regenerationmentioning

confidence: 99%

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In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish

et al. 2019

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Calcium is an important messenger in the neuronal system, but its specific role in axonal regeneration has not been fully investigated. To clarify it, we constructed a noninvasive in vivo calcium‐imaging model of zebrafish Mauthner cells and monitored subcellular calcium dynamics during axonal regeneration. Using the calcium indicator GCamp6f, we observed that the regenerative length correlated with the peak amplitude of the evoked calcium response before axotomy, which suggested that the evoked calcium response might serve as a useful indicator of evoked neuronal activity and axonal regenerative capacity. To investigate this possibility, we overexpressed an inward rectifying potassium channel protein, Kir2.1a, to decrease the Mauthner neuronal activity and found that the inhibition of the calcium response correlated with decreased axonal regeneration. In contrast, treatment of pentylenetetrazol and knockout of the sodium voltage‐gated channel ol subunit 1 gene increased the calcium response and thus enhanced axonal regeneration. Our results therefore increased the understanding of the correlation between the neural activity and the vertebrate axonal regeneration.—Chen, M., Huang, R.‐C, Yang, L.‐Q., Ren, D.‐L., Hu, B. In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish. FASEB J. 33, 7721–7733 (2019). http://www.fasebj.org

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“…For example, axonal expression of N‐cadherin, a cell adhesion molecule that promotes oligodendroglial‐axonal interactions (Schnädelbach et al, ), is increased along axons of the developing zebrafish in an activity dependent manner. Blocking N‐cadherin in turn blocked activity‐induced increases in myelination (Chen et al, ). Social isolation of juvenile mice during a critical period in brain development leads to decreased expression of the axonal ErbB3 ligand Neuregulin‐1 Type III, accompanied by decreased myelination within the prefrontal cortex (Makinodan et al, ).…”

Section: Diffusible and Local Signals In Adaptive Myelinationmentioning

confidence: 99%

Axoglial interactions in myelin plasticity: Evaluating the relationship between neuronal activity and oligodendrocyte dynamics

Foster

Bujalka

Emery

2019

Glia

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Myelin is a critical component of the vertebrate nervous system, both increasing the conduction velocity of myelinated axons and allowing for metabolic coupling between the myelinating cells and axons. An increasing number of studies demonstrate that myelination is not simply a developmentally hardwired program, but rather that new myelinating oligodendrocytes can be generated throughout life. The generation of these oligodendrocytes and the formation of myelin are influenced both during development and adulthood by experience and levels of neuronal activity. This led to the concept of adaptive myelination, where ongoing activity‐dependent changes to myelin represent a form of neural plasticity, refining neuronal functioning, and circuitry. Although human neuroimaging experiments support the concept of dynamic changes within specific white matter tracts relevant to individual tasks, animal studies have only just begun to probe the extent to which neuronal activity may alter myelination at the level of individual circuits and axons. Uncovering the role of adaptive myelination requires a detailed understanding of the localized interactions that occur between active axons and myelinating cells. In this review, we focus on recent animal studies that have begun to investigate the interactions between active axons and myelinating cells and review the evidence for—and against—the ability of neuronal activity to alter myelination at an axon‐specific level.

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N-Cadherin is Involved in Neuronal Activity-Dependent Regulation of Myelinating Capacity of Zebrafish Individual Oligodendrocytes In Vivo

Cited by 22 publications

References 69 publications

The oligodendrocyte growth cone and its actin cytoskeleton: A fundamental element for progenitor cell migration and CNS myelination

The oligodendrocyte growth cone and its actin cytoskeleton: A fundamental element for progenitor cell migration and CNS myelination

In vivo imaging of evoked calcium responses indicates the intrinsic axonal regenerative capacity of zebrafish

Axoglial interactions in myelin plasticity: Evaluating the relationship between neuronal activity and oligodendrocyte dynamics

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