N-Carbon Quantum Dot/Cu Complex for In Vivo Monitoring of Glycine Levels

Raut, Jiko; Sherpa, Rinchen D.; Jana, Santosh K.; Mandal, Santi M.; Mandal, Sukhendu; Hui, Subhra P.; Sahoo, Prithidipa

doi:10.1021/acsanm.3c05401

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…The XPS studies are corroborated by the existence of diverse functional groups having nitrogen and sulfur within the N, S-CDs, which is consistent with the conclusions drawn from the FT-IR analysis. These functional groups accord to the high-quality water solubility of the fluorescent material and facilitate modifications. − …”

Section: Results and Discussionmentioning

confidence: 99%

“…The presence of C–N bonds is indicated by a prominent peak at 1398 cm –1 , which supports the nitrogen doping on the N, S-CQDs. Moreover, The CS group was identified by the peaks seen at around 1079 and 1039 cm –1 , while the C–S and S–H stretching were associated with the bands at 625 and 2143 cm –1 , respectively, indicating sulfur doping. − …”

Section: Results and Discussionmentioning

confidence: 99%

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Development of a Nanomarker for In Vivo Monitoring of Dopamine in Plants

Mondal,

Sarkar,

Raut

et al. 2024

ACS Appl. Bio Mater.

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Dopamine, alongside norepinephrine and epinephrine, belongs to the catecholamine group, widely distributed across both plant and animal kingdoms. In mammals, these compounds serve as neurotransmitters with roles in glycogen mobilization. In plants, their synthesis is modulated in response to stress conditions aiding plant survival by emitting these chemicals, especially dopamine that relieves their resilience against stress caused by both abiotic and biotic factors. In present studies, there is a lack of robust methods to monitor the operations of dopamine under stress conditions or any adverse situations across the plant's developmental stages from cell to cell. In our study, we have introduced a groundbreaking approach to track dopamine generation and activity in various metabolic pathways by using the simple nitrogen and sulfur co-doped carbon quantum dots (N, S-CQDs). These CQDs exhibit dominant biocompatibility, negligible toxicity, and environmentally friendly characteristics using a quenching process for fluorometric dopamine detection. This innovative nanomarker can detect even small amounts of dopamine within plant cells, providing insights into plant responses to strain and anxiety. Confocal microscopy has been used to corroborate this occurrence and to provide visual proof of the process of binding dopamine with these N, S-CQDs inside the cells.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Results and Discussionmentioning

confidence: 99%

Development of a Nanomarker for In Vivo Monitoring of Dopamine in Plants

Mondal,

Sarkar,

Raut

et al. 2024

ACS Appl. Bio Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

A comprehensive review on luminescent carbon dots and their polymer composites: Synthesis to applications

Pandey,

Raikwar

2024

Nano-Structures & Nano-Objects

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In Vivo Monitoring of GABA by N-Doped Carbon Quantum Dots

Raut,

Sherpa,

Jana

et al. 2024

ACS Appl. Nano Mater.

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γ-Aminobutyric acid (GABA) is one of the primary inhibitory neurotransmitters and encompasses 10−30% of the neurons in the CNS. Fundamentally, GABA is responsible for decreasing the nervous system activity by inhibiting the action potential and hence excitotoxicity of neurons. Owing to these neuroregulatory features, GABA has been known to be involved in numerous diseases such as schizophrenia, epilepsy, and other psychiatric conditions. Therefore, from a neuroanatomical as well as a pathological perspective, the visual localization and relative abundance research of neurotransmitters is of great importance. The intricacies of GABA release from neurons are still largely unknown; hence, it will be necessary to analyze endogenous GABA concentrations. Here, we synthesized nitrogen-doped carbon quantum dots (N-CQDs), which employ a turn-off mechanism to detect GABA in vivo. High-resolution transmission electron microscopy (HRTEM), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray spectroscopy (EDX) have been used to verify the structural characteristics and size of the N-CQDs. Using the Stern−Volmer equation, UV−vis and fluorescence spectroscopies, and time-correlated single-photon counting (TCSPC), the mechanism of fluorescence quenching was thoroughly explained. Moreover, 0.16 μM was determined to be the N-CQD detection limit. Furthermore, exogenous GABA in human cell lines and endogenous GABA in the zebrafish forebrain (telencephalon) and midbrain (optic tectum) were both successfully detected by our sensor. Thus, this probe may be used as a competent nanosensor to detect comparative GABA levels among healthy and diseased animal models with GABA neurotransmitter imbalance and neurodegeneration.

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N-Carbon Quantum Dot/Cu Complex for In Vivo Monitoring of Glycine Levels

Cited by 4 publications

References 39 publications

Development of a Nanomarker for In Vivo Monitoring of Dopamine in Plants

Development of a Nanomarker for In Vivo Monitoring of Dopamine in Plants

A comprehensive review on luminescent carbon dots and their polymer composites: Synthesis to applications

In Vivo Monitoring of GABA by N-Doped Carbon Quantum Dots

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