N-carboxymethyl chitosan/sodium alginate composite hydrogel loading plasmid DNA as a promising gene activated matrix for in-situ burn wound treatment

Wang, Litong; Sun, Le; Gu, Zhiyang; Li, Wenya; Guo, Lili; Ma, Saibo; Guo, Lan; Zhang, Wangwang; Han, Baoqin; Chang, Jing

doi:10.1016/j.bioactmat.2021.12.012

Cited by 30 publications

(16 citation statements)

References 65 publications

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“…Biomass materials ( e.g. sodium alginate, 10 carboxymethyl cellulose, 11 etc. ) have attracted the attention of researchers due to their cheap price and ease of degradation.…”

Section: Introductionmentioning

confidence: 99%

Preparation of SA/P(U-AM-ChCl) composite hydrogels by frontal polymerization and its performance study

Hao

et al. 2023

RSC Adv.

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“…Biomass materials ( e.g. sodium alginate, 10 carboxymethyl cellulose, 11 etc. ) have attracted the attention of researchers due to their cheap price and ease of degradation.…”

Section: Introductionmentioning

confidence: 99%

Preparation of SA/P(U-AM-ChCl) composite hydrogels by frontal polymerization and its performance study

Hao

et al. 2023

RSC Adv.

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“…Delivery of nucleic acids such as DNA and RNA is an attractive technique for tissue engineering [ 1 ], wound healing [ 2 ], and gene therapy [ 3 ]. Nucleic acids modulate the expression of functional proteins to induce tissue regeneration and therapeutic effects [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Scaffolds, such as hydrogels [ 2 , 10 ] and nanofibers [ 11 , 12 , 13 , 14 ], with nucleic acids, could be used for gene delivery as an alternative method to eliminate the above issues. These systems are capable of direct and sustained delivery of nucleic acids to cells and tissues [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Gelatin-Based Electrospun Nanofibers Cross-Linked Using Horseradish Peroxidase for Plasmid DNA Delivery

2022

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The delivery of nucleic acids is indispensable for tissue engineering and gene therapy. However, the current approaches involving DNA/RNA delivery by systemic and local injections face issues such as clearance, off-target distribution, and tissue damage. In this study, we report plasmid DNA (pDNA) delivery using gelatin electrospun nanofibers obtained through horseradish peroxidase (HRP)-mediated insolubilization. The nanofibers were obtained through the electrospinning of an aqueous solution containing gelatin possessing phenolic hydroxyl (Ph) moieties (Gelatin-Ph) and HRP with subsequent HRP-mediated cross-linking of the Ph moieties by exposure to air containing 16 ppm H2O2 for 30 min. Then, Lipofectamine/pDNA complexes were immobilized on the nanofibers through immersion in the solution containing the pDNA complexes, resulting in transfection and sustained delivery of pDNA. Cells cultured on the resultant nanofibers expressed genome-editing molecules including Cas9 protein and guide RNA (gRNA), resulting in targeted gene knock-in and knock-out. These results demonstrated the potential of Gelatin-Ph nanofibers obtained through electrospinning and subsequent HRP-mediated cross-linking for gene therapy and tissue regeneration by genome editing.

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“…Polyethylenimine (PEI)-derived polycations as nonviral vectors play an important role in gene delivery, the abundant positively charged amino groups in the polymer structures benefit their DNA binding and further chemical modification. At present, natural polysaccharide modification is a popular strategy to improve the transfection efficiency, biocompatibility, and even specific targeting of polycations. − Sodium alginate, a natural anion polysaccharide obtained from brown algae, is an attractive polymer in biomedical applications because of its biocompatibility, biodegradability, chemical flexibility, and cross-linking capabilities. − Alginate-based hydrogels, − positive charged alginate derivates, and alginate nanospheres − have been developed in gene, protein, and drug delivery, exhibiting high encapsulation and delivery efficiency and reduced toxicity.…”

Section: Introductionmentioning

confidence: 99%

Sodium Alginate-Doping Cationic Nanoparticle As Dual Gene Delivery System for Genetically Bimodal Therapy

Zhan

Chen

et al. 2022

Biomacromolecules

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Photodynamic therapy occupies an important position in cancer therapy because of its minimal invasiveness and high spatiotemporal precision, and photodynamic/gene combined therapy is a promising strategy for additive therapeutic effects. However, the asynchronism and heterogeneity between traditional chemical photosensitizers and nucleic acid would restrict the feasibility of this strategy. KillerRed protein, as an endogenous photosensitizer, could be directly expressed and take effect in situ by transfecting KillerRed reporter genes into cells. Herein, a simple and easily prepared sodium alginate (SA)-doping cationic nanoparticle SA@GP/DNA was developed for dual gene delivery. The nanoparticles could be formed through electrostatic interaction among sodium alginate, polycation, and plasmid DNA. The title complex SA@GP/DNA showed good biocompatibility and gene transfection efficiency. Mechanism studies revealed that SA doping could facilitate the cellular uptake and DNA release. Furthermore, SA@GP/DNA was applied to the codelivery of p53 and KillerRed reporter genes for the synergistic effect combining p53-mediated apoptosis therapy and KillerRed-mediated photodynamic therapy. The ROS generation, tumor cell growth inhibition, and apoptosis assays proved that the dual-gene transfection could mediate the better effect compared with single therapy. This rationally designed dual gene codelivery nanoparticle provides an effective and promising platform for genetically bimodal therapy.

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N-carboxymethyl chitosan/sodium alginate composite hydrogel loading plasmid DNA as a promising gene activated matrix for in-situ burn wound treatment

Cited by 30 publications

References 65 publications

Preparation of SA/P(U-AM-ChCl) composite hydrogels by frontal polymerization and its performance study

Preparation of SA/P(U-AM-ChCl) composite hydrogels by frontal polymerization and its performance study

Gelatin-Based Electrospun Nanofibers Cross-Linked Using Horseradish Peroxidase for Plasmid DNA Delivery

Sodium Alginate-Doping Cationic Nanoparticle As Dual Gene Delivery System for Genetically Bimodal Therapy

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